Application of low temperature microscopy to food systems

1978 ◽  
Vol 112 (2) ◽  
pp. 205-214 ◽  
Author(s):  
E. A. Davis ◽  
J. Gordon
Keyword(s):  
Low Temperature ◽  
Food Systems ◽  
Low Temperature Microscopy

The promises and perfidy of cryomicroscopy and analysis

1994 ◽  
Vol 52 ◽  
pp. 382-383
Author(s):  
Patrick Echlin
Keyword(s):  
Low Temperature ◽  
High Energy ◽  
Short Paper ◽  
List Type ◽  
Time Resolved ◽  
Energy Beam ◽  
Cellular Analysis ◽  
Dissolved Ions ◽  
Embedding Medium ◽  
Low Temperature Microscopy

The unusual title of this short paper and its accompanying tutorial is deliberate, because the intent is to investigate the effectiveness of low temperature microscopy and analysis as one of the more significant elements of the less interventionist procedures we can use to prepare, examine and analyse hydrated and organic materials in high energy beam instruments. The promises offered by all these procedures are well rehearsed and the litany of petitions and responses may be enunciated in the following mantra.Vitrified water can form the perfect embedding medium for bio-organic samples.Frozen samples provide an important, but not exclusive, milieu for the in situ sub-cellular analysis of the dissolved ions and electrolytes whose activities are central to living processes.The rapid conversion of liquids to solids provides a means of arresting dynamic processes and permits resolution of the time resolved interactions between water and suspended and dissolved materials.The low temperature environment necessary for cryomicroscopy and analysis, diminish, but alas do not prevent, the deleterious side effects of ionizing radiation.Sample contamination is virtually eliminated.

The Uptake and Distribution of Potassium in Roots of Duckweed (Lemna Minor L) As Measured using Low Temperature X-Ray Microanalysis

1982 ◽  
Vol 40 ◽  
pp. 408-411
Author(s):  
Patrick Echlin ◽  
Clifford Lai ◽  
Thomas L. Hayes
Keyword(s):  
Low Temperature ◽  
Background Subtraction ◽  
Cell Walls ◽  
Lemna Minor ◽  
Beam Current ◽  
X Ray ◽  
Dark Regime ◽  
Low Temperature Microscopy

Lemna minor L. plants were grown in a 14 H light and 10 H dark regime at 298 K in a medium containing 2 mM [K+]. Roots, 20 mm long and photosynthetically active, were removed and prepared for low temperature microscopy using methods described elsewhere. Fracture faces were made using the AMRAY Biochamber and the analysis carried out using an AMRAY 1000A SEM fitted with an LaB6 gun and a Kevex Si (Li) x-ray detector at 15-17 kV and a beam current of 1-2 nA. A reduced raster (1.0 μm2) was placed at the centre of the cell being analysed, care being taken to avoid any contact with the cell walls. Samples were maintained at 100-110 K throughout analysis and an LN2 cooled anti-contamination device was inserted close to the specimen. The spectra were analysed using the peak/background (P/B) ratio technique following use of background subtraction and peak de-convolution routines. Between eight and twelve fracture faces were analysed for each of the ten sites along the length of the root and the data pooled and presented in Table I.

Low-temperature microscopy and spectroscopy on single defect centers in diamond

1999 ◽  
Vol 60 (16) ◽  
pp. 11503-11508 ◽  
Author(s):  
A. Dräbenstedt ◽  
L. Fleury ◽  
C. Tietz ◽  
F. Jelezko ◽  
S. Kilin ◽  
...  
Keyword(s):  
Low Temperature ◽  
Single Defect ◽  
Defect Centers ◽  
Low Temperature Microscopy

High and Low Temperature Microscopy

1975 ◽  
Vol 47 (14) ◽  
pp. 1279A-1287A
Author(s):  
Walter C. McCrone
Keyword(s):  
Low Temperature ◽  
Low Temperature Microscopy

scholarly journals Involving Resilience In Assessment of The Water-Energy-Food Nexus For Arid And Semiarid Regions

2021 ◽  
Author(s):  
Jesús Manuel Núñez-López ◽  
Brenda Cansino-Loeza ◽  
Xaté Geraldine Sánchez Zarco ◽  
José María Ponce-Ortega
Keyword(s):  
Low Temperature ◽  
Natural Disasters ◽  
Arid Region ◽  
Food Systems ◽  
Systematic Approach ◽  
Future Projections ◽  
Semiarid Regions ◽  
Penalty Costs ◽  
Arid And Semiarid ◽  
Past Conditions

Abstract The proper planning of the water-energy-food nexus is key for urban sustainability. However, the security of water, energy, and food is posed at risk due to uncertain events such as natural disasters. The quantification of resilience in water-energy-food systems has gained relevance in recent years and has served as a key tool to identify vulnerable areas. This work presents a systematic approach to assessing the resilience of the water-energy-food nexus in arid/semiarid regions that present low availability of resources. A methodology for assessing the resilience of the water-energy-food nexus of an arid region is presented, which allows evaluating the system under the disturbances of natural disasters such as hurricanes, low-temperature events, and droughts. The events in which failures in functional services may occur are analyzed using penalty costs. To apply the proposed approach, scenarios corresponding to past conditions and future projections were evaluated for two Mexican arid cities. The results show that it is possible to identify vulnerable areas related to the existence of natural disasters and thereby look for alternatives to maintain the security of the nexus. The proposed approach is general, and it can be applied to other regions with similar characteristics.

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