A critical examination of the electron-microscopical evidence for swollen hydrated cellulose fibrils in green plants

1980 ◽  
Vol 118 (2) ◽  
pp. 177-186 ◽  
Author(s):  
J. H. M. Willison ◽  
R. M. Brown ◽  
S. C. Mueller
Keyword(s):  
Critical Examination ◽  
Green Plants ◽  
Hydrated Cellulose ◽  
Cellulose Fibrils ◽  
Electron Microscopical ◽  
Microscopical Evidence

Investigation of the determinative state of the mouse inner cell mass

Development ◽  
1975 ◽  
Vol 33 (4) ◽  
pp. 991-1001
Author(s):  
J. Rossant
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Number ◽  
Endoderm Differentiation ◽  
Cell Position ◽  
Electron Microscopical ◽  
Microscopical Evidence

Inner cell masses (ICMs) were dissected from 3½- and 4½-day mouse blastocysts and inserted into empty zonae before transfer to the oviducts of pseudopregnant mice. The ICMs survived in the oviduct for at least 2 days with little evidence of reduction in cell number. After 1 day, isolated 3½- day ICMs were still viable, since they could form chimaeras when injected into 3½-day blastocysts. However, no evidence of trophoblast formation could be detected in any of the isolated ICMs, despite exposure of some of their cells to ‘outside’ conditions. This is further evidence that ICM cells, although not overtly differentiated, are determined by 3½ days. Although no trophoblast was formed, 3½-day ICMs formed an outer endoderm layer after 1 day in the oviduct, as judged by light and electron microscopical evidence. It is suggested that cell position may be important in endoderm differentiation.

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