Using conventional fluorescent markers for far-field fluorescence localization nanoscopy allows resolution in the 10-nm range

2009 ◽  
Vol 235 (2) ◽  
pp. 163-171 ◽  
Author(s):  
P. LEMMER ◽  
M. GUNKEL ◽  
Y. WEILAND ◽  
P. MÜLLER ◽  
D. BADDELEY ◽  
...  
Keyword(s):  
Far Field ◽  
Fluorescent Markers ◽  
Nm Range

Possible applications of fraunhofer holography in high resolution electron microscopy

1978 ◽  
Vol 36 (1) ◽  
pp. 222-223 ◽  
Author(s):  
N. Bonnet ◽  
M. Troyon ◽  
P. Gallion
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Transfer Function ◽  
Radiation Damage ◽  
High Resolution Electron Microscopy ◽  
Far Field ◽  
Field Region ◽  
Crystalline Materials ◽  
Resolution Electron ◽  
The Ideal

Two main problems in high resolution electron microscopy are first, the existence of gaps in the transfer function, and then the difficulty to find complex amplitude of the diffracted wawe from registered intensity. The solution of this second problem is in most cases only intended by the realization of several micrographs in different conditions (defocusing distance, illuminating angle, complementary objective apertures…) which can lead to severe problems of contamination or radiation damage for certain specimens.Fraunhofer holography can in principle solve both problems stated above (1,2). The microscope objective is strongly defocused (far-field region) so that the two diffracted beams do not interfere. The ideal transfer function after reconstruction is then unity and the twin image do not overlap on the reconstructed one.We show some applications of the method and results of preliminary tests.Possible application to the study of cavitiesSmall voids (or gas-filled bubbles) created by irradiation in crystalline materials can be observed near the Scherzer focus, but it is then difficult to extract other informations than the approximated size.

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

Multifunctional far-field luminescence nanoscope for studying single molecules and quantum dots

2018 ◽  
Vol 189 (03) ◽  
pp. 312-322 ◽  
Author(s):  
Ivan Yu. Eremchev ◽  
M.Yu. Eremchev ◽  
Andrei V. Naumov
Keyword(s):  
Quantum Dots ◽  
Single Molecules ◽  
Far Field

Conservative Estimation of Whole-body Average SAR in Infant Model for 0.3-6GHz Far-Field Exposure

2009 ◽  
Vol 129 (12) ◽  
pp. 2102-2107 ◽  
Author(s):  
Akimasa Hirata ◽  
Yoshio Nagaya ◽  
Naoki Ito ◽  
Osamu Fujiwara ◽  
Tomoaki Nagaoka ◽  
...  
Keyword(s):  
Whole Body ◽  
Far Field ◽  
Field Exposure ◽  
Conservative Estimation
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