Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from Gram-positive organisms

1992 ◽  
Vol 6 (13) ◽  
pp. 1785-1799 ◽  
Author(s):  
Rudy Antoine ◽  
Camille Locht
Keyword(s):  
Host Range ◽  
Molecular Characterization ◽  
Bordetella Bronchiseptica ◽  
Broad Host Range ◽  
Gram Positive ◽  
Broad Host Range Plasmid ◽  
Sequence Similarities

Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii

Plasmid ◽  
1989 ◽  
Vol 21 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Katty Josson ◽  
Trees Scheirlinck ◽  
Frank Michiels ◽  
Christ Platteeuw ◽  
Patrick Stanssens ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Broad Host Range Plasmid

Characterization of Gram-positive broad host-range plasmids carrying a thermophilic replicon

1991 ◽  
Vol 142 (4) ◽  
pp. 389-396 ◽  
Author(s):  
E De Rossi ◽  
P Brigidi ◽  
M Rossi ◽  
D Matteuzzi ◽  
G Riccardi
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Broad Host Range Plasmids

scholarly journals Molecular analysis and characterization of a broad-host-range plasmid, pEP2.

1994 ◽  
Vol 176 (18) ◽  
pp. 5718-5728 ◽  
Author(s):  
Y Zhang ◽  
J Praszkier ◽  
A Hodgson ◽  
A J Pittard
Keyword(s):  
Host Range ◽  
Molecular Analysis ◽  
Broad Host Range ◽  
Broad Host Range Plasmid

scholarly journals Mobilization Function of the pBHR1 Plasmid, a Derivative of the Broad-Host-Range Plasmid pBBR1

2001 ◽  
Vol 183 (6) ◽  
pp. 2101-2110 ◽  
Author(s):  
Cédric Y. Szpirer ◽  
Michel Faelen ◽  
Martine Couturier
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Gram Negative ◽  
Rolling Circle ◽  
Gram Positive Bacteria ◽  
Broad Host Range Plasmid ◽  
Rolling Circle Mechanism ◽  
Transfer Origin

ABSTRACT The pBHR1 plasmid is a derivative of the small (2.6-kb), mobilizable broad-host-range plasmid pBBR1, which was isolated from the gram-negative bacterium Bordetella bronchiseptica (R. Antoine and C. Locht, Mol. Microbiol. 6:1785–1799, 1992). Plasmid pBBR1 consists of two functional cassettes and presents sequence similarities with the transfer origins of several plasmids and mobilizable transposons from gram-positive bacteria. We show that the Mob protein specifically recognizes a 52-bp sequence which contains, in addition to the transfer origin, the promoter of the mobgene. We demonstrate that this gene is autoregulated. The binding of the Mob protein to the 52-bp sequence could thus allow the formation of a protein-DNA complex with a double function: relaxosome formation andmob gene regulation. We show that the Mob protein is a relaxase, and we located the nic site position in vitro. After sequence alignment, the position of the nic site of pBBR1 corresponds with those of the nick sites of theBacteroides mobilizable transposon Tn4555 and the streptococcal plasmid pMV158. The oriT of the latter is characteristic of a family of mobilizable plasmids that are found in gram-positive bacteria and that replicate by the rolling-circle mechanism. Plasmid pBBR1 thus appears to be a new member of this group, even though it resides in gram-negative bacteria and does not replicate via a rolling-circle mechanism. In addition, we identified two amino acids of the Mob protein necessary for its activity, and we discuss their involvement in the mobilization mechanism.

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