First report of Papaya ringspot virus- W in bottle gourd (Lagenaria siceraria) from India

Luohanguo, Siraitia grosvenorii (Swingle) C. Jeffrey, is a perennial cucurbitaceous plant that is an economically important medicinal and sweetener crop in Guangxi province, China. Surveys conducted during the summer to fall seasons of 2003-2004 in northern Guangxi showed symptoms typical of a viral disease, including leaf mottling, mosaic, vein clearing, curling, and shoestring-like distortion in the field. Mechanical inoculation of sap from leaves of symptomatic plants collected from the surveyed areas caused similar symptoms on tissue culture-derived healthy Luohanguo plants. Two sequences of 0.7 and 1.6 kb with 88 and 97% identity to Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) were amplified using reverse transcription-polymerase chain reaction (RT-PCR) with purified flexuous viral particles or total RNA extracted from the symptomatic Luohanguo leaves as templates with conserved degenerate potyvirus primers (1). To confirm the results, primers specific for PRSV (PP1/PP2, genome coordinates 4064-4083/5087-5069, GenBank Accession No X97251) and ZYMV (ZP1/ZP2, genome coordinates 5540-5557/7937-7920, GenBank Accession No L31350) were used to perform RT-PCR from the same RNA templates. The expected 1.0- and 2.3-kb fragments were amplified and they were 90 and 95% identical to PRSV and ZYMV in sequence, respectively. Watermelon mosaic virus was not detected. To our knowledge, this is the first report of the occurrence of PRSV and ZYMV in Luohanguo. Reference: (1) A. Gibbs et al. J. Virol. Methods 63:9, 1997.

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Detection of Papaya ringspot virus Type W Infecting the Cucurbit Weed Cucumis melo var. dudaim in Florida

Plant Health Progress ◽

10.1094/php-br-13-0126 ◽

2014 ◽

Vol 15 (1) ◽

pp. 29-30 ◽

Cited By ~ 1

Author(s):

Yaowapa Tantiwanich ◽

Carlye A. Baker ◽

William W. Turechek ◽

Scott Adkins

Keyword(s):

Virus Type ◽

Cucumis Melo ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

First Report

This is the first report of Papaya ringspot virus type W infecting Cucumis melo var. dudaim, a cucurbit weed, in Florida. It provides an overview of this virus reservoir for growers, extension workers, crop consultants, and research and regulatory scientists. Accepted for publication 21 January 2014. Published 18 March 2014.

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First report of Epuraea sp. (Coleoptera: Nitidulidae) on bottle gourd (Lagenaria siceraria) in north eastern part of Bangladesh

International Journal of Tropical Insect Science ◽

10.1007/s42690-021-00653-x ◽

2021 ◽

Author(s):

Md. Fuad Mondal ◽

Md. Abu Saleh Abir ◽

Md. Mehedi Hasan ◽

Mahmudul Hasan ◽

Anik Banik

Keyword(s):

Bottle Gourd ◽

Lagenaria Siceraria ◽

First Report ◽

North Eastern ◽

North Eastern Part

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First report and molecular characterization of an isolate of papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba

Bioscience Journal ◽

10.14393/bj-v31n4a2015-26181 ◽

2015 ◽

Vol 31 (4) ◽

pp. 1133-1142 ◽

Cited By ~ 2

Author(s):

Douglas Rodríguez-Martínez ◽

Antonia dos Reis Figueira ◽

Priscilla de Sousa Geraldino Duarte ◽

Suellen Bárbara Ferreira Galvino-Costa ◽

Justo González Olmedo

Keyword(s):

Molecular Characterization ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

First Report

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First report of Papaya ringspot virus infecting papaya in C�te d?Ivoire

Plant Pathology ◽

10.1111/j.1365-3059.2007.01593.x ◽

2007 ◽

Vol 56 (4) ◽

pp. 718-718 ◽

Cited By ~ 3

Author(s):

H. A. Diallo ◽

W. Monger ◽

N. Kouassi ◽

D. T. Yoro ◽

P. Jones

Keyword(s):

Ringspot Virus ◽

Papaya Ringspot Virus ◽

First Report

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First Report of Papaya ringspot virus W in Sponge Gourd from India

Plant Disease ◽

10.1094/pd-90-0974b ◽

2006 ◽

Vol 90 (7) ◽

pp. 974-974 ◽

Cited By ~ 2

Author(s):

Raj Verma ◽

V. K. Baranwal ◽

Satya Prakash ◽

S. P. S. Tomer ◽

R. P. Pant ◽

...

Keyword(s):

Mosaic Virus ◽

Zucchini Yellow Mosaic Virus ◽

Ringspot Virus ◽

Yellow Mosaic Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Natural Occurrence ◽

Papaya Ringspot Virus ◽

First Report ◽

Sponge Gourd ◽

Das Elisa

During August 2004, symptoms resembling a virus disease were observed in commercial cultivation of sponge gourd (Luffa cylindrica (L.) M. Roem. [cv. Chikni]) in Pune, India. Affected plants exhibited mosaic mottling, vein banding, and downward marginal curling on leaves. The incidence of disease was 10 to 30% based on the symptoms and confirmed using enzyme-linked immunosorbent assay (ELISA). Six fields of sponge gourd were visited, and 30 samples were collected randomly. Samples from affected fields were analyzed for the presence of virus by mechanical inoculation. Symptoms typical of those observed in the original field plants appeared 10 days after inoculation on sponge gourd. The virus isolate produced local lesions on Chenopodium amaranticolor and systemic symptoms on Cucumis melo, Cucumis sativus, Cucurbita maxima, Cucurbita pepo, L. acutangula, and L. cylindrica, but did not produce symptoms on Carica papaya (cvs. CO2 and Red Lady), Nicotiana glutinosa, N. tabacum (cv. White Burley), or Vigna unguiculata. Field-infected sponge gourd and all indicator plants were tested using double-antibody sandwich (DAS)-ELISA. The virus was identified as Papaya ringspot virus-W (PRSV-W) using DAS-ELISA (Agdia, Elkhart, IN). A strong, positive reaction was obtained with antiserum to PRSV-W but not with antisera to PRSV-P, Cucumber mosaic virus, Squash mosaic virus, Zucchini yellow mosaic virus, and Groundnut bud necrosis virus. To ensure virus purity, the isolate was passed through three successive single-lesion transfers on C. amaranticolor. Flexuous filamentous particles 775 nm long were observed with electron microscopy of leaf-dip preparation from symptomatic sponge gourd leaves. The virus particles were then decorated with a second PRSV-W antiserum obtained from the Plant Virology Unit, IARI, New Delhi. PRSV-W on sponge gourd has previously been reported from Taiwan (1). To our knowledge, this is the first report of natural occurrence of PRSV-W on sponge gourd in India. Reference: (1.) C. H. Huang et al. J. Agri. Res. China 36:413, 1987.

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First Report of Papaya ringspot virus Infecting Bitter Gourd in China

Plant Disease ◽

10.1094/pdis-01-16-0086-pdn ◽

2016 ◽

Vol 100 (11) ◽

pp. 2338-2338 ◽

Cited By ~ 1

Author(s):

L. Zhu ◽

T. Yang ◽

L. J. Chen ◽

H. H. Lin ◽

D. H. Xi

Keyword(s):

Ringspot Virus ◽

Bitter Gourd ◽

Papaya Ringspot Virus ◽

First Report

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First report of leaf spot of bottle gourd (Lagenaria siceraria) caused by Fusarium equiseti in Pakistan

Journal of Plant Pathology ◽

10.1007/s42161-020-00730-z ◽

2021 ◽

Author(s):

Hafiz Muhammad Usman Aslam ◽

Safdar Ali ◽

Sabin Aslam ◽

Muhammad Amjad Ali ◽

Nasir Ahmad Khan ◽

...

Keyword(s):

Leaf Spot ◽

Bottle Gourd ◽

Lagenaria Siceraria ◽

Fusarium Equiseti ◽

First Report

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First Report of Papaya ringspot virus on Papaya in Iran

Plant Disease ◽

10.1094/pdis.2003.87.9.1148b ◽

2003 ◽

Vol 87 (9) ◽

pp. 1148-1148 ◽

Cited By ~ 2

Author(s):

R. Pourrahim ◽

Sh. Farzadfar ◽

A. R. Golnaraghi ◽

N. Shahraeen

Keyword(s):

Cucurbita Pepo ◽

Potassium Phosphate ◽

Ringspot Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Watermelon Mosaic Virus ◽

Papaya Ringspot Virus ◽

Leaf Extracts ◽

Chenopodium Amaranticolor ◽

First Report ◽

Fruit Samples

Papaya, a popular fruit crop native to the American tropics, was introduced to the southern tropical provinces of Iran in the 1990s and its cultivation is widely increasing in these areas. During April 2000, severe leaf distortion and mottling were observed on papaya trees (Carica papaya) in Hormozgan Province in southern Iran. Affected trees were stunted and yielded less fruit. Samples of papaya leaf extracts (1:10 wt/vol) in 0.01 M potassium phosphate buffer (pH 7.0) were mechanically inoculated on indicator host plants, causing local lesions on Chenopodium amaranticolor and C. quinoa and chlorotic spots followed by systemic mosaic symptoms on Cucurbita pepo. Papaya ringspot virus (PRSV) was detected in the leaf samples of papaya plants and the inoculated Cucurbita pepo plants using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with PRSV-specific antisera (polyclonal antibody AS-0086 and PV-0244, DSMZ, Braunschweig, Germany). PRSV causes one of the most destructive diseases of papaya worldwide (1). PRSV has been previously reported from Citrullus vulgaris and Cucumis melo from Iran as Watermelon mosaic virus 1 (2), but to our knowledge, this is the first report of occurrence of PRSV on papaya in Iran. References: (1) D. E. Purcifull et al. Papaya ringspot virus. No. 292. CMI/AAB, Surrey, England, 1984. (2) F. Ebrahim-Nesbat. Phytopathol. Z. 79:352, 1974.

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