Molecular identification of ‘CandidatusPhytoplasma cynodontis’ associated with Bermuda grass white leaf disease in India

2008 ◽  
Vol 57 (4) ◽  
pp. 770-770 ◽  
Author(s):  
S. K. Snehi ◽  
M. S. Khan ◽  
S. K. Raj ◽  
S. Mall ◽  
M. Singh ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Bermuda Grass ◽  
Leaf Disease ◽  
White Leaf ◽  
White Leaf Disease

scholarly journals First report of a phytoplasma associated with Bermuda-grass white leaf disease in Cuba

2005 ◽  
Vol 54 (2) ◽  
pp. 233-233 ◽  
Author(s):  
Y. Arocha ◽  
D. Horta ◽  
B. Pinol ◽  
I. Palenzuela ◽  
S. Picornell ◽  
...  
Keyword(s):  
Bermuda Grass ◽  
First Report ◽  
Leaf Disease ◽  
White Leaf ◽  
White Leaf Disease

scholarly journals Detection of Bermuda Grass White Leaf Disease in Italy and Characterization of the Associated Phytoplasma by RFLP Analysis

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 862-866 ◽  
Author(s):  
C. Marcone ◽  
A. Ragozzino ◽  
E. Seemüller
Keyword(s):  
Pcr Amplification ◽  
Rflp Analysis ◽  
Bermuda Grass ◽  
Leaf Disease ◽  
White Leaf ◽  
Sugarcane White Leaf ◽  
Group Members ◽  
White Leaf Disease ◽  
Sugarcane White Leaf Phytoplasma ◽  
Fruit Orchards

Bermuda grass showing symptoms of a white leaf disease has been observed in fruit orchards, vegetable fields, and uncultivated areas in the Latium and Campania regions of central and southern Italy. Using polymerase chain reaction (PCR) amplification with phytoplasma-specific primers, all symptomatic plants tested positively; whereas no amplification product was obtained from nonsymptomatic plants. Restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified ribosomal DNA revealed a uniform pattern that was similar to that of the Bermuda grass white leaf phytoplasma collected in Thailand, which is known to be a member of the sugarcane white leaf phytoplasma group. By RFLP analysis, the phytoplasma infecting Bermuda grass could be distinguished from other group members, including the phytoplasmas associated with sugarcane white leaf and Brachiaria white leaf. This is the first report on the presence of the Bermuda grass white leaf phytoplasma in Europe.

scholarly journals First report of a phytoplasma associated with Bermuda grass white leaf disease in Kenya

2010 ◽  
Vol 21 ◽  
pp. 23 ◽  
Author(s):  
E. Obura ◽  
D. Masiga ◽  
C.A.O. Midega ◽  
F. Wachira ◽  
J.A. Pickett ◽  
...  
Keyword(s):  
Bermuda Grass ◽  
First Report ◽  
Leaf Disease ◽  
White Leaf ◽  
White Leaf Disease

scholarly journals Candidates for Symbiotic Control of Sugarcane White Leaf Disease

2012 ◽  
Vol 78 (19) ◽  
pp. 6804-6811 ◽  
Author(s):  
Jureemart Wangkeeree ◽  
Thomas A. Miller ◽  
Yupa Hanboonsong
Keyword(s):  
Developmental Stages ◽  
Cloning And Sequencing ◽  
Content Type ◽  
Leaf Disease ◽  
White Leaf ◽  
Symbiotic Control ◽  
Sugarcane White Leaf ◽  
White Leaf Disease ◽  
Bacterial Genes

ABSTRACTThe leafhopperMatsumuratettix hiroglyphicus(Matsumura) is the most important vector of a phytoplasma pathogen causing sugarcane white leaf (SCWL) disease. The purpose of this study was to evaluate candidate bacterial symbionts for possible use as vehicles in the control of the disease. 16S rRNA bacterial genes were amplified from whole bodies ofM. hiroglyphicusleafhoppers and analyzed by cloning and sequencing. Two dominant groups were found: one belonged to theBetaproteobacteriathat did not closely match any sequences in the database and was named bacterium associated withM. hiroglyphicus(BAMH). Another one found to be abundant in this leafhopper is “CandidatusSulcia muelleri” in the orderBacteroidetes, which was previously reported in the insect members of the Auchenorrhyncha. MostM. hiroglyphicusleafhoppers carry both BAMH and “Ca. Sulcia muelleri.” Fluorescentin situhybridization showed that BAMH and “Ca. Sulcia muelleri” colocalized in the same bacteriomes. BAMH was present in the midgut and ovaries of the leafhopper and was found in all developmental stages, including eggs, nymphs, and adults. Because BAMH appears to be specific for the SCWL vector, we evaluated it as a candidate for symbiotic control of sugarcane white leaf disease.

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