scholarly journals Genomics-based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula

2005 ◽  
Vol 41 (6) ◽  
pp. 875-887 ◽  
Author(s):  
Lahoucine Achnine ◽  
David V. Huhman ◽  
Mohamed A. Farag ◽  
Lloyd W. Sumner ◽  
Jack W. Blount ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Functional Characterization ◽  
Model Legume

Cloning and functional characterization of epidermis-specific promoter MtML1 from Medicago truncatula

2019 ◽  
Vol 300 ◽  
pp. 32-39 ◽  
Author(s):  
Li Gao ◽  
Ye Tian ◽  
Meng-Ci Chen ◽  
Li Wei ◽  
Tian-Ge Gao ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Functional Characterization

scholarly journals Characterization of a dual-affinity nitrate transporter MtNRT1.3 in the model legume Medicago truncatula

2011 ◽  
Vol 62 (15) ◽  
pp. 5595-5605 ◽  
Author(s):  
M.-C. Morere-Le Paven ◽  
L. Viau ◽  
A. Hamon ◽  
C. Vandecasteele ◽  
A. Pellizzaro ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Nitrate Transporter ◽  
Model Legume

Isolation and functional characterization of salt-stress induced RCI2-like genes from Medicago sativa and Medicago truncatula

2015 ◽  
Vol 128 (4) ◽  
pp. 697-707 ◽  
Author(s):  
Ruicai Long ◽  
Fan Zhang ◽  
Zhenyi Li ◽  
Mingna Li ◽  
Lili Cong ◽  
...  
Keyword(s):  
Salt Stress ◽  
Medicago Sativa ◽  
Medicago Truncatula ◽  
Functional Characterization

scholarly journals Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L.

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247170
Author(s):  
Md. Soyib Hasan ◽  
Vishal Singh ◽  
Shiful Islam ◽  
Md. Sifatul Islam ◽  
Raju Ahsan ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Expression Profiling ◽  
Developmental Stages ◽  
Functional Characterization ◽  
Drought Treatment ◽  
Specific Expression ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Genome Wide

Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well as plant development and growth. The present study aimed to a comprehensive genome-wide identification and functional characterization of GST family in one of the economically important legume plants—Medicago truncatula. Here, we have identified a total of ninety-two putative MtGST genes that code for 120 proteins. All these members were classified into twelve classes based on their phylogenetic relationship and the presence of structural conserved domain/motif. Among them, 7 MtGST gene pairs were identified to have segmental duplication. Expression profiling of MtGST transcripts revealed their high level of organ/tissue-specific expression in most of the developmental stages and anatomical tissues. The transcripts of MtGSTU5, MtGSTU8, MtGSTU17, MtGSTU46, and MtGSTU47 showed significant up-regulation in response to various abiotic and biotic stresses. Moreover, transcripts of MtGSTU8, MtGSTU14, MtGSTU28, MtGSTU30, MtGSTU34, MtGSTU46 and MtGSTF8 were found to be highly upregulated in response to drought treatment for 24h and 48h. Among the highly stress-responsive MtGST members, MtGSTU17 showed strong affinity towards its conventional substrates reduced glutathione (GSH) and 1‐chloro‐2,4‐dinitrobenzene (CDNB) with the lowest binding energy of—5.7 kcal/mol and -6.5 kcal/mol, respectively. Furthermore, the substrate-binding site residues of MtGSTU17 were found to be highly conserved. These findings will facilitate the further functional and evolutionary characterization of GST genes in Medicago.

scholarly journals Medicago truncatula possesses a single Shaker outward K+ channel: functional characterization and roles in planta

2019 ◽  
Author(s):  
Alice Drain ◽  
Julien Thouin ◽  
Limin Wang ◽  
Nicolas Pauly ◽  
Manuel Nieves-Cordones ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Root Hair ◽  
Stomatal Closure ◽  
Functional Characterization ◽  
Xylem Sap ◽  
K Channel ◽  
In Planta ◽  
Model Legume ◽  
Rhizobial Symbiosis ◽  
Electrical Signaling

SUMMARYThe model legume Medicago truncatula possesses a single outward Shaker K+ channel, while Arabidopsis thaliana possesses two channels of this type, named SKOR and GORK, the former having been shown to play a major role in K+ secretion into the xylem sap in the root vasculature and the latter to mediate the efflux of K+ across the guard cell membrane upon stomatal closure. Here we show that the expression pattern of the single M. truncatula outward Shaker channel, which has been named MtGORK, includes the root vasculature, guard cells and root hairs. As shown by patch-clamp experiments on root hair protoplasts, besides the Shaker-type slowly-activating outwardly-rectifying K+ conductance encoded by MtGORK, a second K+-permeable conductance, displaying fast activation and weak rectification, can be expressed by M. truncatula. A KO mutation resulting in absence of MtGORK activity is shown to weakly reduce K+ translocation to shoots, and only in plants engaged in rhizobial symbiosis, but to strongly affect the control of stomatal aperture and transpitational water loss. In legumes, the early electrical signaling pathway triggered by Nod Factor perception is known to comprise a short transient depolarization of the root hair plasma membrane. In absence of MtGORK functional expression, while the rate of the membrane repolarization is shown to be decreased by about 3 times, this defect is without any consequence on infection thread development and nodule production, indicating that the plant capacity to engage rhizobial symbiosis does not require integrity of the early electrical signaling events.

scholarly journals Characterization of the Interaction Between the Bacterial Wilt Pathogen Ralstonia solanacearum and the Model Legume Plant Medicago truncatula

2007 ◽  
Vol 20 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Fabienne Vailleau ◽  
Elodie Sartorel ◽  
Marie-Françoise Jardinaud ◽  
Fabien Chardon ◽  
Stéphane Genin ◽  
...  
Keyword(s):  
Plant Species ◽  
Medicago Truncatula ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Recombinant Inbred Lines ◽  
Model Legume ◽  
Legume Plant ◽  
Hrp Genes

The soilborne pathogen Ralstonia solanacearum is the causal agent of bacterial wilt and attacks more than 200 plant species, including some legumes and the model legume plant Medicago truncatula. We have demonstrated that M. truncatula accessions Jemalong A17 and F83005.5 are susceptible to R. solanacearum and, by screening 28 R. solana-cearum strains on the two M. truncatula lines, differential interactions were identified. R. solanacearum GMI1000 infected Jemalong A17 line, and disease symptoms were dependent upon functional hrp genes. An in vitro root inoculation method was employed to demonstrate that R. solanacearum colonized M. truncatula via the xylem and intercellular spaces. R. solanacearum multiplication was restricted by a factor greater than 1 × 105 in the resistant line F83005.5 compared with susceptible Jemalong A17. Genetic analysis of recombinant inbred lines from a cross between Jemalong A17 and F83005.5 revealed the presence of major quantitative trait loci for bacterial wilt resistance located on chromosome 5. The results indicate that the root pathosystem for M. truncatula will provide useful traits for molecular analyses of disease and resistance in this model plant species.

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