scholarly journals Benzoyl-arginine naphthylamide (BANA) hydrolysis by Treponema denticola and/or Bacteroides gingivalis in periodontal plaques

2007 ◽  
Vol 5 (5) ◽  
pp. 275-279 ◽  
Author(s):  
W. A. Bretz ◽  
D. E. Lopatin ◽  
W. J. Loesche
Keyword(s):  
Treponema Denticola ◽  
Bacteroides Gingivalis

Identification of Periodontopathic Bacteria Based Upon their Peptidase Activities

1988 ◽  
Vol 2 (2) ◽  
pp. 304-309 ◽  
Author(s):  
H. Suido ◽  
T. Eguchi ◽  
T. Tanaka ◽  
M. Nakamura
Keyword(s):  
Rapid Detection ◽  
Periodontal Diseases ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Peptidase Activity ◽  
Identification Methods ◽  
Periodontopathic Bacteria ◽  
Periodontal Tissues ◽  
Detection And Identification ◽  
Bacteroides Gingivalis

Black-pigmented Bacteroides (BPB) and spirochetes are associated with some forms of periodontal diseases. The enzymes produced by these bacteria may participate in the destruction of gingival and periodontal tissues. Certain proteases and peptidases are unique to Bacteroides gingivalis and Treponema denticola. Our purpose was to study the peptidases of periodontopathogens and to evaluate the use of unique peptidases for detection and identification of these bacteria. Bacteria used were BPB, Treponema, Fusobacterium, Capnocytophaga, Actinobacillus (Haemophilus), and Eikenella species. Twenty-five substrates, including mono-, di-, and tri-peptides of β-naphthylamide (β-NA) were employed for examination of peptidase activity. Clinically isolated BPB were obtained from 16 adult periodontitis patients. One hundred and ninety-three BPB strains were identified by conventional identification methods, and the peptidase activity was determined with N-Carbobenzoxy-glycyl-glycyl-L-arginine-β-naphthylamide (N-CBz-Gly-Gly-Arg-β-NA) used as a substrate. Among tested periodontopathic bacteria, only B. gingivalis and T. denticola could strongly hydrolyze some substrates such as N-CBz-Gly-Gly-Arg-β-NA and N-Benzoyl-L-valyl-glycyl-L-arginine-4-methoxy-(3-naphthylamide (Bz-Val-Gly-Arg-β-NA). In subgingival plaque samples, all patients showed BPB, and eight out of 16 patients possessed B. gingivalis by culture. One hundred and ten strains out of 193 BPB isolated were identified as B. gingivalis. Ninety-nine percent of these B. gingivalis strains identified showed N-CBz-Gly-Gly-Arg-β-NAhydrolyzing activity on a newly developed colorimetric plate assay. However, none of the other strains showed this activity in cultures of subgingival plaque which did not allow growth of spirochetes. Enzymes, such as N-CBz-Gly-Gly-Arg-peptidase and Bz-Val-Gly-Arg-peptidase, specific for B. gingivalis and T. denticola seem to be useful for rapid detection and identification of these bacteria.

Exemplar Abstract for Treponema denticola (ex Brumpt 1925) Chan et al. 1993 emend. Hördt et al. 2020.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Treponema Denticola

Exemplar Abstract for Treponema denticola (ex Brumpt 1925) Chan et al. 1993 emend. Hördt et al. 2020.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Treponema Denticola

Exemplar Abstract for Treponema denticola (ex Brumpt 1925) Chan et al. 1993 emend. Hördt et al. 2020.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Treponema Denticola

The effects of anti-Bacteroides gingivalis(Bg), and anti-Actinobacillus actinomycetemcomitans(Aa) antibodies on Bg and Aa.

1989 ◽  
Vol 31 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kazuhito HORINO ◽  
Keiichi SATOH ◽  
Hiromasa YOSHIE ◽  
Kohji HARA
Keyword(s):  
Actinobacillus Actinomycetemcomitans ◽  
Bacteroides Gingivalis

Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens

2018 ◽  
Vol 68 (12) ◽  
pp. 2853-2856 ◽  
Author(s):  
Igor Jelihovschi ◽  
Cristian Drochioi ◽  
Aida Corina Badescu ◽  
Raoul Vasile Lupusoru ◽  
Alexandra Elena Munteanu ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Pcr Analysis ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Periodontal Pathogens ◽  
Radiographic Evidence ◽  
Gingival Bleeding ◽  
As Species ◽  
Qpcr Quantification

The diagnosis of periodontal disease is mainly based on use of clinical and radiographic evidence. In this study we employed a quantitative PCR analysis of Aggregatibacter actinomycetemcomitans and Treponema denticola as species strongly involved in periodontal diseases, burden in periodontal pockets to detect the main sampling factors that interfere with qPCR results. From 22 patients with advanced periodontal disease, subgingival plaque was comparatively collected by paper points and periodontal Gracey curettes. Samples were collected from the same situs in presence of gingival bleeding and absence of bleeding. The concordance and agreement of results between samples were assessed. The present study demonstrates that subgingival plaque sampling with sterile absorbable paper points is often accompanied by gingival bleeding resulting in quantification biases of periodontal pathogens.

Cleavage of Human Immunoglobulin G by Treponema denticola

Anaerobe ◽  
2001 ◽  
Vol 7 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Daniel Grenier ◽  
Denis Mayrand
Keyword(s):  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Treponema Denticola ◽  
Human Immunoglobulin G

Development of an FhbB based chimeric vaccinogen that elicits antibodies that block Factor H binding and cleavage by the periopathogen Treponema denticola

2020 ◽  
Author(s):  
Nathaniel S. O'Bier ◽  
Dhara T. Patel ◽  
Lee D. Oliver ◽  
Daniel P. Miller ◽  
Richard T. Marconi
Keyword(s):  
Factor H ◽  
Treponema Denticola ◽  
Block Factor

Identification of a fliG homologue in treponema denticola

Gene ◽  
1995 ◽  
Vol 161 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Hollis F. Heinzerling ◽  
Jana E.C. Penders ◽  
Robert A. Burne
Keyword(s):  
Treponema Denticola
Sign in / Sign up

Export Citation Format

Share Document