Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens

2018 ◽  
Vol 68 (12) ◽  
pp. 2853-2856 ◽  
Author(s):  
Igor Jelihovschi ◽  
Cristian Drochioi ◽  
Aida Corina Badescu ◽  
Raoul Vasile Lupusoru ◽  
Alexandra Elena Munteanu ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Pcr Analysis ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Periodontal Pathogens ◽  
Radiographic Evidence ◽  
Gingival Bleeding ◽  
As Species ◽  
Qpcr Quantification

The diagnosis of periodontal disease is mainly based on use of clinical and radiographic evidence. In this study we employed a quantitative PCR analysis of Aggregatibacter actinomycetemcomitans and Treponema denticola as species strongly involved in periodontal diseases, burden in periodontal pockets to detect the main sampling factors that interfere with qPCR results. From 22 patients with advanced periodontal disease, subgingival plaque was comparatively collected by paper points and periodontal Gracey curettes. Samples were collected from the same situs in presence of gingival bleeding and absence of bleeding. The concordance and agreement of results between samples were assessed. The present study demonstrates that subgingival plaque sampling with sterile absorbable paper points is often accompanied by gingival bleeding resulting in quantification biases of periodontal pathogens.

scholarly journals Review- The periodontal pathogen Treponema denticola: an atherosclerosis risk factor

2021 ◽  
Vol 10 (1) ◽  
pp. e25810111637
Author(s):  
Pâmela Beatriz do Rosário Estevam dos Santos ◽  
Patrícia Michelle Nagai de Lima ◽  
Ana Luiza do Rosário Palma ◽  
Amjad Abu Hasna ◽  
Rodnei Dennis Rossoni ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Systemic Disease ◽  
Periodontal Pathogen ◽  
In Vivo Studies ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Atherosclerosis Risk Factor

Objective: Treponema denticola “T. denticola” is a pathogen associated with periodontal diseases that exhibits capacity for adherence, invasion, and colonization of host tissues, which allows alternating its location and damage in different sites of human body. This review aimed to discuss different studies that detected T. denticola in atherosclerotic plaques, demonstrating the importance of periodontal disease on the systemic health and the necessity of exploring the outcome of this colonization apart from the oral cavity. Methodology: Fifty-five studies were identified and gathered in this review according to the following topics: Periodontal disease, atherosclerosis and T. denticola. In vitro and in vivo studies published between 2002 and 2020 were searched on PubMed, raising relevant insights about the role of T. denticola and its association with the systemic disease, atherosclerosis, focusing on the bacterial tissue invasion and development of atherosclerosis. Results: After bibliographic review, it was possible to identify studies demonstrating the presence of T. denticola and other oral pathogens in cardiac or vascular tissues and in blood serum, as well, there is research in which other evidence of a relationship with atherosclerosis is shown. Conclusion: The invasion of periodontal pathogens and its toxins associated to the host’s immune and inflammatory response may contribute to the development of atherosclerosis.

Detection of Pathogen-Related Oral Spirochetes, Treponema denticola, and Treponema socranskii in Dental Plaque from Dogs

1996 ◽  
Vol 13 (4) ◽  
pp. 135-138 ◽  
Author(s):  
George R. Riviere ◽  
Aaron J. Thompson ◽  
Randi D. Brannan ◽  
Donald E. McCoy ◽  
Lloyd G. Simonson
Keyword(s):  
Monoclonal Antibodies ◽  
Dental Plaque ◽  
Periodontal Diseases ◽  
Representative Specimen ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Reference Sites ◽  
Periodontal Health ◽  
Periodontal Status ◽  
Periodontal Condition

Spirochetes have been observed in dental plaque from dogs, but specific spirochetes have not been identified. In particular, it is not known whether treponemes associated with periodontal diseases in humans also occur in dogs, and whether, like in humans, detection of specific treponemes correlates with periodontal status of dogs. Forty-two dogs were grouped according to the worst periodontal condition in the mouth, as determined by overt signs of inflammation and pocket probing depths. A representative specimen of dental plaque was obtained by pooling subgingival plaque collected from three uniform reference sites, irrespective of periodontal status at selected sites. The presence of pathogen-related oral spirochetes, Treponema denticola, and T. socranskii was determined using specific monoclonal antibodies in an immunocytochemical microscopic assay. All three treponemes were detected in all groups, but a significantly greater proportion of dogs with pocket probing depths ≥5 mm had detectable treponemes, compared to dogs that were in periodontal health.

scholarly journals MICRO BIOLOGY OF PERIODONTAL DISEASE- A REVIEW

2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Jageer Chinna ◽  
Jannat Sharma
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Disease Process ◽  
Fusobacterium Nucleatum ◽  
Host Cells ◽  
Bacterial Invasion ◽  
Periodontal Pathogens ◽  
Tissue Destruction ◽  
Campylobacter Rectus ◽  
Periodontal Tissues

Periodontal diseases are inflammatory and destructive diseases of the dentogingival complex associated with specific periodontal pathogens inhabiting periodontal pockets. Periodontal diseases lead to damage of the periodontal tissues supporting the teeth (bone and connective tissue) and affect the quality of life of the affected individuals: poor alimentation, tooth loss, social and financial problems. Although it is generally considered that the disease has multifactorial etiology, data show that some specific Gram-negative microorganisms in the subgingival plaque biofilm play a major role in the initiation and progression of periodontitis. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia form a consortium in the subgingival biofilm and are regarded as the principal periodontopathogenic bacteria. Other microorganisms that have been implicated as predominant species in the disease process are: Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Campylobacter rectus, Peptostreptococcus migros, Eikenella corrodens. In periodontitis, the initiation of the disease is the colonization of the tissues by these pathogenic species. The next step is bacterial invasion or invasion by pathogenic products into the periodontal tissues, interactions of bacteria or their substances with host cells, and this directly/indirectly causes degradation of the periodontium, resulting in tissue destruction. Keywords: periodontal disease, periodontal pathogens, microbiology.

Identification of Periodontopathic Bacteria Based Upon their Peptidase Activities

1988 ◽  
Vol 2 (2) ◽  
pp. 304-309 ◽  
Author(s):  
H. Suido ◽  
T. Eguchi ◽  
T. Tanaka ◽  
M. Nakamura
Keyword(s):  
Rapid Detection ◽  
Periodontal Diseases ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Peptidase Activity ◽  
Identification Methods ◽  
Periodontopathic Bacteria ◽  
Periodontal Tissues ◽  
Detection And Identification ◽  
Bacteroides Gingivalis

Black-pigmented Bacteroides (BPB) and spirochetes are associated with some forms of periodontal diseases. The enzymes produced by these bacteria may participate in the destruction of gingival and periodontal tissues. Certain proteases and peptidases are unique to Bacteroides gingivalis and Treponema denticola. Our purpose was to study the peptidases of periodontopathogens and to evaluate the use of unique peptidases for detection and identification of these bacteria. Bacteria used were BPB, Treponema, Fusobacterium, Capnocytophaga, Actinobacillus (Haemophilus), and Eikenella species. Twenty-five substrates, including mono-, di-, and tri-peptides of β-naphthylamide (β-NA) were employed for examination of peptidase activity. Clinically isolated BPB were obtained from 16 adult periodontitis patients. One hundred and ninety-three BPB strains were identified by conventional identification methods, and the peptidase activity was determined with N-Carbobenzoxy-glycyl-glycyl-L-arginine-β-naphthylamide (N-CBz-Gly-Gly-Arg-β-NA) used as a substrate. Among tested periodontopathic bacteria, only B. gingivalis and T. denticola could strongly hydrolyze some substrates such as N-CBz-Gly-Gly-Arg-β-NA and N-Benzoyl-L-valyl-glycyl-L-arginine-4-methoxy-(3-naphthylamide (Bz-Val-Gly-Arg-β-NA). In subgingival plaque samples, all patients showed BPB, and eight out of 16 patients possessed B. gingivalis by culture. One hundred and ten strains out of 193 BPB isolated were identified as B. gingivalis. Ninety-nine percent of these B. gingivalis strains identified showed N-CBz-Gly-Gly-Arg-β-NAhydrolyzing activity on a newly developed colorimetric plate assay. However, none of the other strains showed this activity in cultures of subgingival plaque which did not allow growth of spirochetes. Enzymes, such as N-CBz-Gly-Gly-Arg-peptidase and Bz-Val-Gly-Arg-peptidase, specific for B. gingivalis and T. denticola seem to be useful for rapid detection and identification of these bacteria.

scholarly journals An Overview of Gingival and Periodontal Diseases in 12 to 15 years using Gingivitis and Periodontitis Site Prevalence Index (WHO, 1978)

2011 ◽  
Vol 2 (3) ◽  
pp. 175-181
Author(s):  
Anand Shigli ◽  
Shobha D Deshpande ◽  
PV Ravindranath Reddy
Keyword(s):  
Socioeconomic Status ◽  
Periodontal Disease ◽  
Children And Adolescents ◽  
Preventive Measures ◽  
Periodontal Diseases ◽  
Betel Nut ◽  
Prevalence Index ◽  
Gingival Bleeding ◽  
Betel Nut Chewing ◽  
Subgingival Calculus

ABSTRACT The aim of the study was to overview the gingival and periodontal disease in children and adolescents aged 12 to 15 years in Belgaum city. Visible plaque, gingival bleeding, supragingival and subgingival calculus and periodontal pockets were measured. Examination of buccal, lingual and mesial areas of each tooth was recorded sequentially in both arches using Gingivitis and Periodontitis Site Prevalence Index (WHO, 1978). The prevalence of gingival and periodontal disease was more in males than females and in Hindu population compared to others. The sites affected by gingivitis and periodontitis increased with the age. Periodontal problems were significantly more prevalent in patients with betel nut chewing habit. Visible plaque was most common while periodontal pockets were least common in the adolescents. Age, socioeconomic status, brushing habits and brushing frequency did not affect much to the prevalence of sites of gingivitis and periodontal disease. It was concluded that early diagnosis and preventive measures would surely reduce the growing problem of gingival and periodontal diseases among the children and adolescents.

scholarly journals DIAGNOSTIC CRITERIA IN THE PLANNING OF ORTHOPEDIC TREATMENT OF PATIENTS WITH INFLAMMATORY PERIODONTAL DISEASES

2019 ◽  
Vol 15 (2) ◽  
pp. 102-105 ◽  
Author(s):  
Евгений Жулев ◽  
Evgeniy Zhulev ◽  
Алёна Кочубейник ◽  
Alena Kochubeynik ◽  
Наталья Круглова ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Clinical Examination ◽  
Periodontal Diseases ◽  
Main Role ◽  
Frequency Of Occurrence ◽  
Periodontal Pathogens ◽  
Orthopedic Treatment ◽  
Clinical Conditions ◽  
Simultaneous Identification ◽  
Degrees Of Severity

Subject. Currently, about 530 types of microorganisms that live in supragingival and subgingival plaque have been discovered. To periodontal pathogens today carry a little more than ten types. The main role in the occurrence of periodontal disease is played by microorganisms included in the “red complex” of BANA+: Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia. With the simultaneous identification of these types can be judged on the high risk of developing periodontal disease. Objective ― calculating the frequency of occurrence of various types of dentition defects of patients with varying severity of periodontal disease, establishing the connection between the severity of periodontal disease and the number of periodontal pathogenic microflora. Methodology. A clinical examination of a patients group which consists of 32 women and 10 men in the age from 38 to 66 years was conducted. Anamnesis was collected using a survey and a special questionnaire. External examination and examination of the oral cavity allowed to find the clinical conditions and anatomical features that leads to periodontal disease’s development. Results. Based on the data of clinical and radiological examination, the patients were divided into three groups according to the severity of periodontal disease. During the clinical examination, we calculated the frequency of occurrence of various types of dentition defects in patients with varying degrees of severity of periodontal disease. Findings. It has been found that the course of chronic generalized periodontitis depends on the number of types of aggressive forms of periodontal pathogens.

scholarly journals The Utility of the BANA Test for Monitoring Anaerobic Infections due to Spirochetes (Treponema denticola) in Periodontal Disease

1990 ◽  
Vol 69 (10) ◽  
pp. 1696-1702 ◽  
Author(s):  
W.J. Loesche ◽  
J. Giordano ◽  
P.P. Hujoel
Keyword(s):  
Periodontal Disease ◽  
Initial Therapy ◽  
Treponema Denticola ◽  
Subgingival Plaque ◽  
Scaling And Root Planing ◽  
Anaerobic Infection ◽  
Plaque Sample ◽  
Root Planing ◽  
Anaerobic Infections ◽  
Bana Test

Treponema denticola, Porphyromonas gingivalis, and Bacteroides forsythus each possesses an enzyme(s) that hydrolyzes the synthetic substrate benzoyl-DL-arginine-naphthylamide (BANA). The presence of these organisms in a subgingival plaque sample can be determined by the ability of the plaque to hydrolyze BANA. In the present study, we describe the usefulness of the BANA test at various stages of a clinical trial of the efficacy of metronidazole in the treatment of periodontal disease. A BANA-positive test was significantly associated with high levels and proportions of spirochetes in the plaque, so that it provided information comparable with that which could be obtained by a microscopic examination of the plaque. Patients with such anaerobic spirochetal infections were randomly assigned to a group receiving either metronidazole or placebo (250 mg, three times a day) for one week and whose teeth were scaled and root-planed. The advantages of the decision that metronidazole be used were apparent from the comparison with the results obtained in the patients who received only the scaling and root planing. The initially BANA-positive teeth in the patients treated with metronidazole, scaling, and root planing gained attachment and exhibited a significant reduction in the need for periodontal surgery, when compared with the BANA-positive teeth in the patients who received only placebo, scaling, and root planing. After the conclusion of this therapy, those teeth with persistent BANA-positive plaques had significantly higher proportions and levels of spirochetes than did the teeth with BANA-negative plaques. A tooth site which tested BANA-positive after the initial therapy lost significantly more attachment in the year following active treatment than did a tooth site that tested BANA-negative, i.e., a mean difference of 0.48 mm per year. The ability of the BANA test to detect an anaerobic infection due to the BANA-positive species and possibly to predict future attachment loss indicates that this test may be helpful in the management of the periodontally diseased patient.

scholarly journals Porphyromonas gingivalisandTreponema denticolaMixed Microbial Infection in a Rat Model of Periodontal Disease

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Raj K. Verma ◽  
Sunethra Rajapakse ◽  
Archana Meka ◽  
Clayton Hamrick ◽  
Sheela Pola ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Rat Model ◽  
Alveolar Bone ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Microbial Infection ◽  
Morphometric Measurements ◽  
Th2 Immune Response ◽  
Microbial Infections ◽  
Antibody Levels

Porphyromonas gingivalisandTreponema denticolaare periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis thatP. gingivalisandT. denticolaare synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with eitherP. gingivalisorT. denticolaor mixed microbial infections for 7 and 12 weeks.P. gingivalisgenomic DNA was detected more frequently by PCR thanT. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed thatP. gingivalisandT. denticolaexhibit no synergistic virulence in a rat model of periodontal disease.

scholarly journals Detection of oral spirochete (Treponema denticola) by modified Fontana staining technique from sub-gingival plaques of patients suffering from periodontal disease and to analyse the morphology of spirochetes with respect to the disease’s status

2020 ◽  
Vol 6 (8) ◽  
pp. 286
Author(s):  
Sunil Paudel ◽  
Jyoti M. Nagamoti ◽  
Ved Prakash Mishra ◽  
Suraj Twanabasu ◽  
Susan Kusma
Keyword(s):  
Periodontal Disease ◽  
Screening Method ◽  
Staining Technique ◽  
Laboratory Diagnosis ◽  
Global Scale ◽  
Personal Hygiene ◽  
Treponema Denticola ◽  
Periodontal Pathogens ◽  
Periodontal Tissues ◽  
Periodontal Infection

<p class="abstract"><strong>Background:</strong> Infections pertaining to mankind particularly those affecting the periodontal tissues are of serious concerns worldwide and are increasing on a global scale particularly in the tropical and subtropical countries like India. The clinical presentation, though very typical of periodontal infection, is often confused with other oral infection, making laboratory diagnosis and confirmation necessary. The management of periodontal infections needs personal hygiene, awareness of infection, proper diagnosis and medication. The present study was undertaken to demonstrate the oral spirochete (<em>Treponema denticola</em>) from periodontal pathogens suffering from periodontal disease.</p><p class="abstract"><strong>Methods:</strong> A total of 55 clinically diagnosed selected patients of periodontal infection attending the outpatient Department of Periodontics, V. K. Institute of Dental Science, KLE University, Belgaum were studied. Dental plaque was taken as specimens from the patients were processed by modified Fontana staining and observed in microscope.  </p><p class="abstract"><strong>Results:</strong> A total number of 55 plaque samples were stained by modified Fontana staining technique. 30 cases were positive and 25 cases were negative. Males were affected more as compared to the females and the age group ranging from 15 to 65. Farmers were more affected compared to other occupation.</p><p class="abstract"><strong>Conclusions:</strong> Microscopic method by modified silver nitrate staining can be a very useful screening method for evaluation of oral spirochetes in a clinical setting if used judiciously keeping in mind the variables that can affect the results.</p>

scholarly journals INVESTIGATION OF SPECIES COMPOSITION OF MICROFLORA IN PERIODONTAL POCKETS IN PATIENTS WITH GENERALIZED PERIODONTITIS AND METABOLIC SYNDROME

2021 ◽  
Vol 21 (3) ◽  
pp. 147-150
Author(s):  
T.A. Hlushchenko
Keyword(s):  
Metabolic Syndrome ◽  
Periodontal Disease ◽  
Species Composition ◽  
Periodontal Diseases ◽  
Oral Microbiome ◽  
Periodontal Pathogens ◽  
Periodontal Tissues ◽  
The Metabolic Syndrome ◽  
Microbial Invasion ◽  
Rank One

Among dental diseases, periodontal diseases rank one of the leading places and are considered as the most pressing issues of modern dentistry. The presence of concomitant somatic pathology, in particular, cardiovascular, endocrinological, autoimmune diseases is an important factor that considerably determines the course and prognosis of periodontal disease. Metabolic syndrome is regarded as an urgent social and medical issue due to its high prevalence among the general population and its contribution to the development and progression of cardiovascular disease. The number of reports and scientific interest in the metabolic syndrome has grown up significantly in recent years, but despite the significant number of studies, the oral microbiome in patients with periodontal disease and underlying metabolic syndrome is still remaining insufficiently studied. The aim of this work was to investigate the species composition of the microflora in periodontal pockets and the frequency of excretion of certain types of microorganisms in the acute generalized periodontitis in patients with metabolic syndrome. A microbiological study was performed in 30 people with metabolic syndrome and generalized periodontitis, who formed the main group, and in 30 people with generalized periodontitis without endocrinological pathology, who formed a comparison group. The results of microbiological examination indicate pathological changes in the oral microbiome in the patients with metabolic syndrome demonstrating a predominance of periodontal pathogens. It can be assumed that the components of the metabolic syndrome can initiate and support microbial invasion thus resulting in the inflammatory reaction of periodontal tissues. There is a similarity between pathogenetic mechanisms of metabolic syndrome and periodontal disease that lead to the impairment of all types of metabolism: protein, lipid, mineral, carbohydrate. As a consequence, this contributes to the progressive destruction of oral tissues. The obtained data enable to suggest the dependence between the presence of the patient's metabolic syndrome and the development of intensive damage to periodontal tissues.

Sign in / Sign up

Export Citation Format

Share Document