Comparative mitogenicity and polyclonal B cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-BALB/c) and thymic (BALB/c) mice

1992 ◽  
Vol 7 (2) ◽  
pp. 71-77 ◽  
Author(s):  
K. Sveen ◽  
N. Skaug
Keyword(s):  
B Cell ◽  
Cell Activation ◽  
B Cell Activation ◽  
Spleen Cells ◽  
Bacterial Lipopolysaccharides ◽  
Activation Capacity

scholarly journals Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation.

1990 ◽  
Vol 171 (1) ◽  
pp. 265-292 ◽  
Author(s):  
M Shlomchik ◽  
M Mascelli ◽  
H Shan ◽  
M Z Radic ◽  
D Pisetsky ◽  
...  
Keyword(s):  
Immune Responses ◽  
B Cell ◽  
Cell Activation ◽  
Specific Antigen ◽  
B Cell Activation ◽  
Spleen Cells ◽  
Systemic Autoimmune Diseases ◽  
Proximate Cause ◽  
Arginine Residues ◽  
Dna Antibodies

The proximate cause of autoantibodies characteristic of systemic autoimmune diseases has been controversial. One hypothesis is that autoantibodies are the result of polyclonal nonspecific B cell activation. Alternatively, autoantibodies could be the result of antigen-driven B cell activation, as observed in secondary immune responses. We have approached this question by studying monoclonal anti-DNA autoantibodies derived from unmanipulated spleen cells of the autoimmune MRL/lpr mouse strain. This analysis shows that anti-DNAs, like rheumatoid factors (19), are the result of specific antigen-driven stimulation. In addition, correlation of sequences with fine specificity shows that: (a) somatic mutations can cause specificity for dsDNA and that such mutations are selected for; (b) arginine residues play an important role in determining specificity; and (c) anti-idiotypes that recognize the majority of anti-DNA are probably not specific for any one family of V regions.

scholarly journals Experimental amyloidosis: the inducer is a polyclonal B-cell activator to which susceptibility is under genetic control.

1975 ◽  
Vol 142 (6) ◽  
pp. 1564-1569 ◽  
Author(s):  
S Britton
Keyword(s):  
B Cell ◽  
Genetic Control ◽  
Polyclonal Antibody ◽  
Cell Activation ◽  
B Cell Activation ◽  
Spleen Cells ◽  
Antibody Synthesis ◽  
Experimental Amyloidosis ◽  
Spleen Lymphocytes ◽  
Cell Activator

Experimental amyloidosis in mice can be induced by repeated injections of casein. It has now been demonstrated that casein induces strong polyclonal antibody synthesis in mouse B spleen lymphocytes. This effect is much more pronounced in spleen cells from anyloid-susceptible mice (CBA/J) than amyloid-resistant mice (A/J). It is suggested that amyloidosis can be due in some instances to a constant exposure for molecules which induce polyclonal B-cell activation.

scholarly journals B-cell activation by lipopolysaccharide. Distinct pathways for induction of mitosis and antibody production.

1977 ◽  
Vol 146 (6) ◽  
pp. 1640-1647 ◽  
Author(s):  
M K Hoffmann ◽  
C Galanos ◽  
S Koenig ◽  
H F Oettgen
Keyword(s):  
T Cells ◽  
Immune System ◽  
B Cell ◽  
Tumor Necrosis ◽  
Cell Activation ◽  
Regulatory Mechanisms ◽  
Helper T Cells ◽  
B Cell Activation ◽  
Spleen Cells ◽  
Cell Mitosis

The role played by macrophages in two effects of lipopolysaccharide (LPS) on the immune system of the mouse-substitution for helper T cells and induction of B-cell mitosis-has been investigated. C3H/HeJ mice are unresponsive and do not produce (as other strains do) antibody to 2,4,6-trinitrophenol (TNP) conjugated with autologous mouse erythrocytes (MRBC-TNP) in the presence of LPS. We found that C3H/HeJ spleen cells produce antibody to MRBC-TNP when (a) LPS and macrophages from LPS-responsive C3HeB/FeJ mice or (b) tumor necrosis serum ([TNS] induced by LPS in responsive mice) are added. The mitotic response was not restored. The findings suggest that adjuvanticity and mitogenicity represent distinct pathways of B-cell activation by LPS, subject to different regulatory mechanisms.

scholarly journals SPECIFIC MURINE B-CELL ACTIVATION BY SYNTHETIC SINGLE- AND DOUBLE-STRANDED POLYNUCLEOTIDES

1973 ◽  
Vol 138 (6) ◽  
pp. 1545-1563 ◽  
Author(s):  
Irwin Scher ◽  
Douglas M. Strong ◽  
Aftab Ahmed ◽  
Richard C. Knudsen ◽  
Kenneth W. Sell
Keyword(s):  
Molecular Structure ◽  
B Cell ◽  
Cell Activation ◽  
Thymidine Incorporation ◽  
Inbred Strains ◽  
B Cell Activation ◽  
Spleen Cells ◽  
Functional Interaction ◽  
Murine Spleen ◽  
Cell Mitogen

The synthetic single- and double-stranded polynucleotides, poly I, poly C, and poly I·C, were shown to induce thymidine incorporation in six inbred strains of murine spleen cells. This stimulation was shown to be secondary to B-cell activation and not due to contamination of the polynucleotides with bacterial lipopolysaccharide (LPS). The ability of poly I·C to act as a B-cell mitogen, in addition to its behavior as a thymic-independent antigen, suggested that these two phenomena may be related. The similarity of the molecular structure of poly I·C to LPS, a material which also acts as a thymic-independent antigen and a B-cell mitogen, supports the hypothesis that the polyvalent nature of these materials accounts for their functional interaction with murine B cells.

B-Cell Activation by Helper T-Cell Membranes

1994 ◽  
Vol 14 (3-4) ◽  
pp. 221-238 ◽  
Author(s):  
Marilyn R. Kehry ◽  
Philip D. Hodgkin
Keyword(s):  
T Cell ◽  
B Cell ◽  
Cell Membranes ◽  
Cell Activation ◽  
B Cell Activation ◽  
Helper T Cell
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