Isolation of Mesophyll Protoplasts of Nicotiana rustica and Their Regeneration into Plants Flowering in vitro

1979 ◽  
Vol 47 (1) ◽  
pp. 7-10 ◽  
Author(s):  
RAVINDER GILL ◽  
A. RASHID ◽  
S. C. MAHESHWARI
Planta ◽  
1967 ◽  
Vol 72 (4) ◽  
pp. 355-370 ◽  
Author(s):  
Colette Nitsch ◽  
J. P. Nitsch

1984 ◽  
Vol 75 (4) ◽  
pp. 1034-1039 ◽  
Author(s):  
Yves Meyer ◽  
Lorette Aspart ◽  
Yvette Chartier

1990 ◽  
Vol 92 (2) ◽  
pp. 520-527 ◽  
Author(s):  
Jean Grosset ◽  
Yves Meyer ◽  
Yvette Chartier ◽  
Serge Kauffmann ◽  
Michel Legrand ◽  
...  

HortScience ◽  
1990 ◽  
Vol 25 (4) ◽  
pp. 474-477 ◽  
Author(s):  
W. Msikita ◽  
R.M. Skirvin ◽  
J.A. Juvik ◽  
W.E. Splittstoesser ◽  
N. Ali

Seeds of `Burpless Hybrid' cucumber (Cucumis sativus L.) were separated from their seedcoats and excised into pieces comprising an embryonic axis and two cotyledons. The seed pieces were disinfested and explanted on modified Murashige and Skoog medium with 16 combinations of BAP (2.0, 3.0, 4.0, 5.0 mg·liter-1) and NAA (0.0,0.1,0.2,0.3 mg·liter-1). On all media, embryonic axes germinated and grew into whole plants within 4 weeks, but did not flower. Cotyledons developed adventitious shoots and male flowers on most media. A few female flowers developed. The best shoot regeneration and the most female flowers were observed on medium with 2.0 mg BAP and 0.3 mg NAA/liter. One of the female flowers was pollinated in vitro and it developed a small fruit with viable seed.


Planta ◽  
1967 ◽  
Vol 72 (4) ◽  
pp. 371-384 ◽  
Author(s):  
Colette Nitsch ◽  
J. P. Nitsch

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