Isolation and characterization of catalytic and calmodulin-binding domains of Bordetella pertussis adenylate cyclase

1991 ◽  
Vol 196 (2) ◽  
pp. 469-474 ◽  
Author(s):  
Helene MUNIER ◽  
Anne-Marie GILLES ◽  
Philippe GLASER ◽  
Evelyne KRIN ◽  
Antoine DANCHIN ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Bordetella Pertussis ◽  
Calmodulin Binding ◽  
Binding Domains ◽  
Isolation And Characterization

scholarly journals Characterization of the Calmodulin-binding and of the Catalytic Domains of Bordetella pertussis Adenylate Cyclase

1989 ◽  
Vol 264 (7) ◽  
pp. 4015-4020
Author(s):  
D Ladant ◽  
S Michelson ◽  
R Sarfati ◽  
A M Gilles ◽  
R Predeleanu ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Bordetella Pertussis ◽  
Calmodulin Binding ◽  
Catalytic Domains

scholarly journals Characterization of a synthetic calmodulin-binding peptide derived from Bacillus anthracis adenylate cyclase.

1993 ◽  
Vol 268 (3) ◽  
pp. 1695-1701
Author(s):  
H. Munier ◽  
F.J. Blanco ◽  
B. Prêcheur ◽  
E. Diesis ◽  
J.L. Nieto ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Bacillus Anthracis ◽  
Binding Peptide ◽  
Calmodulin Binding

Characterization of ATP and calmodulin-binding properties of a truncated form of Bacillus anthracis adenylate cyclase

Biochemistry ◽  
1990 ◽  
Vol 29 (20) ◽  
pp. 4922-4928 ◽  
Author(s):  
Elisabeth Labruyere ◽  
Michele Mock ◽  
Daniel Ladant ◽  
Susan Michelson ◽  
Anne Marie Gilles ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Bacillus Anthracis ◽  
Truncated Form ◽  
Binding Properties ◽  
Calmodulin Binding

Isolation and characterization of collagen-binding domains from human von Willebrand factor

2009 ◽  
Vol 24 (3) ◽  
pp. 150-154 ◽  
Author(s):  
N. E. Sharapova ◽  
A. P. Kotnova ◽  
Z. M. Galushkina ◽  
N. N. Poletaeva ◽  
N. V. Lavrova ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Collagen Binding ◽  
Binding Domains ◽  
Isolation And Characterization ◽  
Von Willebrand ◽  
Willebrand Factor

Bordetella pertussis adenylate cyclase: purification and characterization of the toxic form of the enzyme.

1989 ◽  
Vol 8 (9) ◽  
pp. 2755-2760 ◽  
Author(s):  
A. Rogel ◽  
J.E. Schultz ◽  
R.M. Brownlie ◽  
J.G. Coote ◽  
R. Parton ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Bordetella Pertussis ◽  
Purification And Characterization

scholarly journals Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms

Science ◽  
2020 ◽  
Vol 370 (6519) ◽  
pp. 950-957 ◽  
Author(s):  
M. Alejandra Tortorici ◽  
Martina Beltramello ◽  
Florian A. Lempp ◽  
Dora Pinto ◽  
Ha V. Dang ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Viral Escape ◽  
Angiotensin Converting Enzyme 2 ◽  
Effector Functions ◽  
Human Antibodies ◽  
Binding Domains ◽  
Isolation And Characterization ◽  
Cryo Electron Microscopy ◽  
Escape Mutants

Efficient therapeutic options are needed to control the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that has caused more than 922,000 fatalities as of 13 September 2020. We report the isolation and characterization of two ultrapotent SARS-CoV-2 human neutralizing antibodies (S2E12 and S2M11) that protect hamsters against SARS-CoV-2 challenge. Cryo–electron microscopy structures show that S2E12 and S2M11 competitively block angiotensin-converting enzyme 2 (ACE2) attachment and that S2M11 also locks the spike in a closed conformation by recognition of a quaternary epitope spanning two adjacent receptor-binding domains. Antibody cocktails that include S2M11, S2E12, or the previously identified S309 antibody broadly neutralize a panel of circulating SARS-CoV-2 isolates and activate effector functions. Our results pave the way to implement antibody cocktails for prophylaxis or therapy, circumventing or limiting the emergence of viral escape mutants.

Sign in / Sign up

Export Citation Format

Share Document