scholarly journals Solution Structure of the DNA-Binding Domain of the Tomato Heat-Stress Transcription Factor HSF24

1996 ◽  
Vol 236 (3) ◽  
pp. 911-921 ◽  
Author(s):  
Jurgen Schultheiss ◽  
Olaf Kunert ◽  
Uwe Gase ◽  
Klaus-Dieter Scharf ◽  
Lutz Nover ◽  
...  
2000 ◽  
Vol 296 (2) ◽  
pp. 351-359 ◽  
Author(s):  
Maria J.P van Dongen ◽  
Anna Cederberg ◽  
Peter Carlsson ◽  
Sven Enerbäck ◽  
Mats Wikström

1994 ◽  
Vol 1 (9) ◽  
pp. 605-614 ◽  
Author(s):  
Geerten W. Vuister ◽  
Soon-Jong Kim ◽  
András Orosz ◽  
John Marquardt ◽  
Carl Wu ◽  
...  

Biochemistry ◽  
2001 ◽  
Vol 40 (20) ◽  
pp. 5861-5869 ◽  
Author(s):  
Johan Weigelt ◽  
Isabel Climent ◽  
Karin Dahlman-Wright ◽  
Mats Wikström

2004 ◽  
Vol 16 (12) ◽  
pp. 3448-3459 ◽  
Author(s):  
Kazuhiko Yamasaki ◽  
Takanori Kigawa ◽  
Makoto Inoue ◽  
Masaru Tateno ◽  
Tomoko Yamasaki ◽  
...  

Biochemistry ◽  
2004 ◽  
Vol 43 (51) ◽  
pp. 16027-16035 ◽  
Author(s):  
Shinichiro Oka ◽  
Yasuhisa Shiraishi ◽  
Takuya Yoshida ◽  
Tadayasu Ohkubo ◽  
Yukio Sugiura ◽  
...  

2004 ◽  
Vol 30 (2) ◽  
pp. 211-214 ◽  
Author(s):  
Alejandro D. Nadra ◽  
Tommaso Eliseo ◽  
Yu-Keung Mok ◽  
Fabio C.L. Almeida ◽  
Mark Bycroft ◽  
...  

2017 ◽  
Author(s):  
Jungeui Hong ◽  
Nathan Brandt ◽  
Ally Yang ◽  
Tim Hughes ◽  
David Gresham

Understanding the molecular basis of gene expression evolution is a central problem in evolutionary biology. However, connecting changes in gene expression to increased fitness, and identifying the functional basis of those changes, remains challenging. To study adaptive evolution of gene expression in real time, we performed long term experimental evolution (LTEE) of Saccharomyces cerevisiae (budding yeast) in ammonium-limited chemostats. Following several hundred generations of continuous selection we found significant divergence of nitrogen-responsive gene expression in lineages with increased fitness. In multiple independent lineages we found repeated selection for non-synonymous mutations in the zinc finger DNA binding domain of the activating transcription factor (TF), GAT1, that operates within incoherent feedforward loops to control expression of the nitrogen catabolite repression (NCR) regulon. Missense mutations in the DNA binding domain of GAT1 reduce its binding affinity for the GATAA consensus sequence in a promoter-specific manner, resulting in increased expression of ammonium permease genes via both direct and indirect effects, thereby conferring increased fitness. We find that altered transcriptional output of the NCR regulon results in antagonistic pleiotropy in alternate environments and that the DNA binding domain of GAT1 is subject to purifying selection in natural populations. Our study shows that adaptive evolution of gene expression can entail tuning expression output by quantitative changes in TF binding affinities while maintaining the overall topology of a gene regulatory network.


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