Cross-resistance of Cry1Ab-selected Asian corn borer to other Cry toxins

2010 ◽  
Vol 134 (5) ◽  
pp. 429-438 ◽  
Author(s):  
L. Xu ◽  
Z. Wang ◽  
J. Zhang ◽  
K. He ◽  
N. Ferry ◽  
...  
Keyword(s):  
Cross Resistance ◽  
Cry Toxins ◽  
Corn Borer ◽  
Asian Corn Borer

scholarly journals Genetic Basis of Cry1F-Resistance in a Laboratory Selected Asian Corn Borer Strain and Its Cross-Resistance to Other Bacillus thuringiensis Toxins

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0161189 ◽  
Author(s):  
Yueqin Wang ◽  
Yidong Wang ◽  
Zhenying Wang ◽  
Alejandra Bravo ◽  
Mario Soberón ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Genetic Basis ◽  
Cross Resistance ◽  
Corn Borer ◽  
Asian Corn Borer

scholarly journals Evolution of Asian Corn Borer Resistance to Bt Toxins Used Singly or in Pairs

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 461 ◽  
Author(s):  
Yueqin Wang ◽  
Yudong Quan ◽  
Jing Yang ◽  
Changlong Shu ◽  
Zhenying Wang ◽  
...  
Keyword(s):  
Transgenic Crops ◽  
Model System ◽  
Cross Resistance ◽  
Ostrinia Furnacalis ◽  
Worst Case ◽  
Corn Borer ◽  
Laboratory Selection ◽  
Bt Toxins ◽  
Asian Corn Borer ◽  
Evolution Of Resistance

Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) have revolutionized pest control, but the benefits of this approach have been reduced by the evolution of resistance in pests. The widely adopted ’pyramid strategy’ for delaying resistance entails transgenic crops producing two or more distinct toxins that kill the same pest. The limited experimental evidence supporting this strategy comes primarily from a model system under ideal conditions. Here we tested the pyramid strategy under nearly worst-case conditions, including some cross-resistance between the toxins in the pyramid. In a laboratory selection experiment with an artificial diet, we used Bt toxins Cry1Ab, Cry1F, and Cry1Ie singly or in pairs against Ostrinia furnacalis, one of the most destructive pests of corn in Asia. Under the conditions evaluated, pairs of toxins did not consistently delay the evolution of resistance relative to single toxins.

scholarly journals Characterization of the Cry1Ah resistance in Asian corn Borer and its cross-resistance to other Bacillus thuringiensis toxins

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Muhammad Zeeshan Shabbir ◽  
Yudong Quan ◽  
Zhenying Wang ◽  
Alejandra Bravo ◽  
Mario Soberón ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cross Resistance ◽  
Corn Borer ◽  
Asian Corn Borer

scholarly journals Analysis of Cry1Ah Toxin-Binding Reliability to Midgut Membrane Proteins of the Asian Corn Borer

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 418
Author(s):  
Sivaprasath Prabu ◽  
Muhammad Zeeshan Shabbir ◽  
Zhenying Wang ◽  
Kanglai He
Keyword(s):  
Binding Proteins ◽  
Healing Process ◽  
Wound Healing Process ◽  
Agricultural Pests ◽  
Cry Toxins ◽  
Corn Borer ◽  
Toxin Binding ◽  
Pathogen Invasion ◽  
Cry Toxin ◽  
Asian Corn Borer

Evolution of insect resistance to Bt toxins challenges the use of Cry toxins to control agricultural pests. In lepidopterans, Cry toxin affinity towards multiple midgut epithelial receptors has become a matter of dispute. Cry1Ah toxin-binding proteins were identified in the larval midgut of susceptible (ACB-BtS) and resistant (ACB-AhR) strains of the Asian corn borer (ACB). A pull-down assay was performed using biotinylated Cry1Ah toxin, and the binding proteins were identified by employing liquid chromatography–tandem mass spectrometry (LC-MS/MS). This study aimed to find the binding consistency of the midgut epithelial protein to the Cry1Ah toxin. The binding proteins from different fractions of SDS-PAGE showed a different pattern. We observed an isoform of prophenoloxidase PPO1b (UniProt Acc No. A0A1Q1MKI0), which was found only in the ACB-AhR fractions. Prophenoloxidase (proPO) is an extraordinary defense molecule activated in insect species during pathogen invasion and the wound healing process. Importantly, this prophenoloxidase might have direct/indirect interaction with the Cry1Ah toxin. Our data also suggest that factors like techniques, enrichment of binding proteins in the sample and the reversible and irreversible nature of the brush border membrane vesicles (BBMVs) to Cry toxins could cause the inconsistency in the protein–protein interactions. Moreover, inside the larva midgut, the influence of the Cry toxins under physiological conditions might be different from the laboratory procedures.

Selection for Cry1F resistance in the European corn borer and cross-resistance to other Cry toxins

2008 ◽  
Vol 126 (2) ◽  
pp. 115-121 ◽  
Author(s):  
Eliseu J.G. Pereira ◽  
Bruce A. Lang ◽  
Nicholas P. Storer ◽  
Blair D. Siegfried
Keyword(s):  
European Corn Borer ◽  
Cross Resistance ◽  
Cry Toxins ◽  
Corn Borer ◽  
Selection For

scholarly journals Trichogramma ostriniae Is More Effective Than Trichogramma dendrolimi As a Biocontrol Agent of the Asian Corn Borer, Ostrinia furnacalis

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 70
Author(s):  
Yu Wang ◽  
Yang-Yang Hou ◽  
Giovanni Benelli ◽  
Nicolas Desneux ◽  
Asad Ali ◽  
...  
Keyword(s):  
Biocontrol Agent ◽  
Developmental Time ◽  
Economic Losses ◽  
Ostrinia Furnacalis ◽  
Female Progeny ◽  
Corn Borer ◽  
High Preference ◽  
Asian Corn Borer ◽  
Biocontrol Potential ◽  
Trichogramma Dendrolimi

The Asian corn borer (ACB), Ostrinia furnicalis, is a serious corn pest in south-east Asia, causing huge economic losses every year. Trichogramma dendrolimi and Trichogramma ostriniae, two egg parasitoids, have previously been identified as key biological control agents. To determine the age impact of ACB eggs on their effective biocontrol potential, herein we compared the biological parameters (i.e., number of parasitized eggs, emergence, developmental time, and sex ratio) of both parasitoids on ACB eggs of various ages (i.e., 0–4, 4–8, 8–12, 12–16, 16–24, 24–36, and 36–48 h old), respectively. Our results showed that the age of ACB eggs had a significant impact on the parasitization activity of T. dendrolimi in both choice and no-choice conditions. Trichogramma dendrolimi preferred to parasitize 0–8-h-old ACB eggs, and its parasitization dramatically declined on ACB eggs older than 8 h under choice and no-choice conditions. On the other hand, T. ostriniae showed high preference to parasitize all tested ACB egg ages. The age of ACB eggs had no significant impact on the parasitization of T. ostriniae under choice and no-choice conditions. Furthermore, the female progeny of T. dendrolimi decreased as the age of ACB increased, while no differences were found in female progeny of T. ostriniae. Trichogramma ostriniae also developed faster on each ACB egg age group in comparison with T. dendrolimi. Overall, the age of ACB eggs had a significant impact on T. dendrolimi performance, leading us to conclude that T. ostriniae is more effective than T. dendrolimi as a biocontrol agent of the ACB.

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