Modification of mesangial cell function by ambient chloride is absent in Dahl salt sensitive rat

Nephrology ◽  
1997 ◽  
Vol 3 (6) ◽  
pp. 463-469
Author(s):  
Mamiko OHARA ◽  
Toshihiro OKUDA ◽  
Yoji INISHI ◽  
Kiyoshi KUROKAWA
Keyword(s):  
Cell Function ◽  
Mesangial Cell

UTP and ATP induce different membrane voltage responses in rat mesangial cells

1997 ◽  
Vol 272 (6) ◽  
pp. F704-F711 ◽  
Author(s):  
M. Huber-Lang ◽  
K. G. Fischer ◽  
J. Gloy ◽  
P. Schollmeyer ◽  
A. Kramer-Guth ◽  
...  
Keyword(s):  
Cell Function ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Membrane Voltage ◽  
Nucleotide Receptors ◽  
Ion Currents ◽  
Sodium Gluconate ◽  
Reactive Blue 2 ◽  
Whole Cell ◽  
Sustained Increase

UTP and ATP induce different membrane voltage responses in rat mesangial cells. Recent studies have indicated that UTP and ATP might modulate mesangial cell function in a different manner. Here we compared the effect of UTP and ATP on membrane voltage (Vm) and ion currents in mesangial cells in primary culture, and we examined whether different nucleotide receptors are involved. In patch-clamp experiments in the fast whole cell configuration, UTP (in contrast to ATP) caused a sustained and concentration-dependent depolarization (half-maximal effective dose, 10(-5) M), but ATP caused only a transient depolarization. During the depolarization, UTP induced a sustained increase of the whole cell conductance (Gm), whereas ATP induced only a transient increase of Gm. When cells were dialyzed with Cs2SO4 and extracellular Cl- was replaced by 145 mM sodium gluconate, addition of UTP or ATP (both 10(-4) M) did not significantly increase Gm. Addition of ATP in the presence of UTP caused an additional depolarization by 5 mV, which was followed by a hyperpolarization by 21 mV. Repetitive application of ATP led to an attenuation of the ATP-induced depolarization. Then, in the presence of ATP, UTP still induced a significant depolarization by 10 mV. Suramine and reactive blue 2 did not inhibit the depolarization induced by UTP, but these inhibited the Vm response to ATP. In microfluorescence experiments, UTP and ATP caused a concentration-dependent increase of the intracellular calcium activity ([Ca2+]i) in mesangial cells. Application of both UTP and ATP had no additive effect on [Ca2+]i. The results suggest that mesangial cells possess, in addition to P2y purinoceptors, separate nucleotide receptors for UTP.

Modification of mesangial cell function by chloride is attenuated in spontaneously hypertensive rats

1994 ◽  
Vol 266 (4) ◽  
pp. F586-F591 ◽  
Author(s):  
T. Okuda ◽  
Y. Inishi ◽  
T. Arakawa ◽  
M. Ohara ◽  
K. Kurokawa
Keyword(s):  
Spontaneously Hypertensive Rats ◽  
Cell Function ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Pge2 Production ◽  
Ang Ii ◽  
Hypertensive Rats ◽  
Cell Contraction ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto

The effects of extracellular Cl- concentration ([Cl-]o) on cultured mesangial cells from spontaneously hypertensive rats (SHR) were examined. Angiotensin II (ANG II)- and vasopressin (VP)-induced cell contraction and Ca2+ transients of SHR mesangial cells were unaffected when the cells were preincubated with 10 mM [Cl-]o, while obvious suppression of the responses to these agents was observed in Wistar-Kyoto (WKY) mesangial cells. Enhanced prostaglandin E2 (PGE2) production was elicited by a decrease in [Cl-]o in WKY mesangial cells. In contrast, PGE2 synthesis by SHR mesangial cells was not enhanced by low [Cl-]o. However, ANG II-stimulated PGE2 production and the attenuation of ANG II-induced cell contraction and Ca2+ transients by the addition of exogenous PGE2 were present equally in both WKY and SHR mesangial cells. Based on these findings, we conclude that the absence of modification of mesangial cell function by [Cl-]o in SHR is due to the inability of low [Cl-]o to enhance PGE2 production. Insensitivity of SHR mesangial cells to changes in [Cl-]o might underlie the dysregulation of renal function in SHR.

scholarly journals Glomerular Dysfunction in the Aging Fischer 344 Rat Is Associated With Excessive Growth and Normal Mesangial Cell Function

1996 ◽  
Vol 51A (2) ◽  
pp. M80-M85 ◽  
Author(s):  
G. F. McDermott ◽  
A. Ingram ◽  
J. Scholey ◽  
J. L. Kirkland ◽  
C. I. Whiteside
Keyword(s):  
Cell Function ◽  
Mesangial Cell ◽  
Fischer 344 ◽  
Fischer 344 Rat

Regulation of Mesangial Cell Function by Thrombin

1994 ◽  
Vol 20 (04) ◽  
pp. 333-338 ◽  
Author(s):  
William Glass ◽  
Dean Troyer ◽  
Jeffrey Kreisberg
Keyword(s):  
Cell Function ◽  
Mesangial Cell

scholarly journals Regulation of mesangial cell function by PKD2

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Juan Du ◽  
Sherry Sours ◽  
Min Ding ◽  
Rong Ma
Keyword(s):  
Cell Function ◽  
Mesangial Cell

scholarly journals Establishment of conditionally immortalized human glomerular mesangial cells in culture, with unique migratory properties

2011 ◽  
Vol 301 (5) ◽  
pp. F1131-F1138 ◽  
Author(s):  
Ramadan M. Sarrab ◽  
Rachel Lennon ◽  
Lan Ni ◽  
Matthew D. Wherlock ◽  
Gavin I. Welsh ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Biology ◽  
Cell Function ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Wilms Tumor ◽  
Glomerular Mesangial Cells ◽  
Glomerular Mesangial Cell ◽  
Glomerular Cell ◽  
Human Mesangial Cell

The aim of this study was to establish an immortalized human mesangial cell line similar to mesangial cells in vivo for use as a tool for understanding glomerular cell function. Mesangial cells were isolated from glomerular outgrowths from a normal human kidney, then retrovirally transfected with a temperature-sensitive SV40T antigen+human telomerase (hTERT). Mesangial cells exhibited features of compact cells with small bodies in a confluent monolayer at 33°C, but the cell shape changed to flat and stellate after 5 days in growth-restrictive conditions (37°C). Western blot and immunofluorescence analysis showed that podocyte markers (nephrin, CD2AP, podocin, Wilms' tumor-1) and an endothelial-specific molecule (VE-cadherin) were not detectable in this cell line, whereas markers characteristic of mesangial cells (α-SMA, fibronectin, and PDGFβ-R) were strongly expressed. In migration assays, a significant reduction in wound surface was observed in podocyte and endothelial cells as soon as 12 h (75 and 62%, respectively) and complete wound closure after 24 h. In contrast, no significant change was observed in mesangial cells after 12 h, and even after 48 h the wounds were not completely closed. Until now, conditionally immortalized podocyte and endothelial cell lines derived from mice and humans have been described, and this has greatly boosted research on glomerular physiology and pathology. We have established the first conditionally immortalized human glomerular mesangial cell line, which will be an important adjunct in studies of representative glomerular cells, as well as in coculture studies. Unexpectedly, mesangial cells' ability to migrate seems to be slower than for other glomerular cells, suggesting this line will demonstrate functional properties distinct from previously available mesangial cell cultures. This conditionally immortalized human mesangial cell line represents a new tool for the study of human mesangial cell biology in vitro.

Effects of Dexamethasone on Cultured Mesangial Cell Function During Phagocytosis

Nephrology ◽  
1984 ◽  
pp. 609-620
Author(s):  
Laurent Baud ◽  
Joelle Perez ◽  
Diego Pujol ◽  
Raymond Ardaillou
Keyword(s):  
Cell Function ◽  
Mesangial Cell

scholarly journals Transcriptomic and Proteomic Profiling Provides Insight into Mesangial Cell Function in IgA Nephropathy

2017 ◽  
Vol 28 (10) ◽  
pp. 2961-2972 ◽  
Author(s):  
Peidi Liu ◽  
Emelie Lassén ◽  
Viji Nair ◽  
Celine C. Berthier ◽  
Miyuki Suguro ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Cell Function ◽  
Mesangial Cell ◽  
Proteomic Profiling ◽  
Insight Into

Freeze-substitution of rye grass and ragweed pollen grains

1990 ◽  
Vol 48 (3) ◽  
pp. 686-687
Author(s):  
Liza B. Martinez ◽  
Susan M. Wick
Keyword(s):  
Cell Function ◽  
Pollen Grains ◽  
Freeze Substitution ◽  
Cell Types ◽  
Rapid Freezing ◽  
Rye Grass ◽  
Acrylic Resins ◽  
Allergenic Proteins ◽  
Cold Embedding ◽  
Structural Preservation

Rapid freezing and freeze-substitution have been employed as alternatives to chemical fixation because of the improved structural preservation obtained in various cell types. This has been attributed to biomolecular immobilization derived from the extremely rapid arrest of cell function. These methods allow the elimination of conventionally used fixatives, which may have denaturing or “masking” effects on proteins. Thus, this makes them ideal techniques for immunocytochemistry, in which preservation of both ultrastructure and antigenicity are important. These procedures are also compatible with cold embedding acrylic resins which are known to increase sensitivity in immunolabelling.This study reveals how rapid freezing and freeze-substitution may prove to be useful in the study of the mobile allergenic proteins of rye grass and ragweed. Most studies have relied on the use of osmium tetroxide to achieve the necessary ultrastructural detail in pollen whereas those that omitted it have had to contend with poor overall preservation.

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