Evidence of a Monosynaptic Pathway Between Cells of the Ventromedial Medulla and the Motoneuron Pool of the Thoracic Spinal Cord in Rat: Electron Microscopic Analysis of Synaptic Contacts

1991 ◽  
Vol 3 (1) ◽  
pp. 55-65 ◽  
Author(s):  
A. Zagon ◽  
S. J. Bacon
Keyword(s):  
Spinal Cord ◽  
Microscopic Analysis ◽  
Electron Microscopic Analysis ◽  
Electron Microscopic ◽  
Thoracic Spinal Cord ◽  
Synaptic Contacts ◽  
Motoneuron Pool ◽  
Thoracic Spinal ◽  
Ventromedial Medulla

Activity of 2′, 3′-Cyclic Nucleotide 3′-Phosphodiesterase (CNPase) in Spinal Cord with Spongy Change Induced by a Single Oral Dose of Aniline in Rats

2010 ◽  
Vol 38 (3) ◽  
pp. 359-365 ◽  
Author(s):  
Takeshi Kanno ◽  
Tetsuro Kurotaki ◽  
Naoaki Yamada ◽  
Kotaro Yamashita ◽  
Yumi Wako ◽  
...  
Keyword(s):  
Spinal Cord ◽  
White Matter ◽  
Cyclic Nucleotide ◽  
Electron Microscopic Examination ◽  
Ultrastructural Level ◽  
Electron Microscopic ◽  
Thoracic Spinal Cord ◽  
Thoracic Spinal ◽  
Old Rats ◽  
Rough Endoplasmic Reticula

A spongy change in the spinal cord white matter was observed in four-week-old rats treated with aniline. Although this change was found to be a result of the myelin sheath splitting at the ultrastructural level, the mechanism is unknown. This study was conducted to identify the mechanism of the spongy change in aniline-treated rats. The spongy change in the spinal cord white matter was first detected on day 5 in the histopathologic examination. The incidence and severity of the lesions, especially in the lateral and ventral funiculi of the thoracic spinal cord white matter, increased prominently from day 8 to day 10. In all rats, immunohistochemical staining by anti-2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) occurred along the cytoplasmic boundaries of the normal oligodendroglia. However, mild to moderate anti-CNPase staining extended to the swollen cytoplasm of the oligodendroglia in the aniline-treated rats from day 2 to day 4. In the electron microscopic examination, free ribosomes and rough endoplasmic reticula in the cytoplasm of the oligodendroglia increased on days 3 and 4. These changes were considered to be related to CNPase expression. However, CNPase expression decreased, whereas the spongy changes were detected from day 5. The reduction in CNPase expression may contribute to the changes in the myelin morphology observed in aniline intoxication.

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