scholarly journals The vomeronasal organ of the tammar wallaby

2008 ◽  
Vol 213 (2) ◽  
pp. 93-105 ◽  
Author(s):  
Nanette Y. Schneider ◽  
Terence P. Fletcher ◽  
Geoff Shaw ◽  
Marilyn B. Renfree
Keyword(s):  
Vomeronasal Organ ◽  
Tammar Wallaby

scholarly journals Goα Expression in the Vomeronasal Organ and Olfactory Bulb of the Tammar Wallaby

2012 ◽  
Vol 37 (6) ◽  
pp. 567-577 ◽  
Author(s):  
Nanette Y. Schneider ◽  
Terrence P. Fletcher ◽  
Geoff Shaw ◽  
Marilyn B. Renfree
Keyword(s):  
Olfactory Bulb ◽  
Vomeronasal Organ ◽  
Tammar Wallaby

scholarly journals Does a Third Model for the Vomeronasal Processing of Information Exist? Insights from the Macropodid Neuroanatomy

2021 ◽  
Author(s):  
Mateo V. TORRES ◽  
Irene ORTIZ-LEAL ◽  
Paula R. VILLAMAYOR ◽  
Andrea FERREIRO ◽  
José Luis ROIS ◽  
...  
Keyword(s):  
Vomeronasal Organ ◽  
Tammar Wallaby ◽  
Vomeronasal System ◽  
Mammal Species ◽  
Α Subunit ◽  
Ulex Europaeus ◽  
Vomeronasal Receptors ◽  
Uniform Model ◽  
Segregated Model ◽  
Degree Of Differentiation

Abstract The study of the α-subunit of Gi2 and Go proteins in the accessory olfactory bulb (AOB) was crucial for the identification of the two main families of vomeronasal receptors, V1R and V2R. Both families are expressed in the rodent and lagomorph AOBs, according to a segregated model characterized by topographical anteroposterior zonation. Many mammal species have suffered from the deterioration of the Gαo pathway and are categorized as belonging to the uniform model. This scenario has been complicated by characterization of the AOB in the tammar wallaby, Macropus eugenii, which appears to follow a third model of vomeronasal organization featuring exclusive Gαo protein expression, referred to as the intermediate model, which has not yet been replicated in any other species. Our morphofunctional study of the vomeronasal system (VNS) in Bennett’s wallaby, Macropus rufogriseus, provides further information regarding this third model of vomeronasal transduction.A comprehensive histological, lectin, and immunohistochemical study of the Bennett’s wallaby VNS was performed. Anti-Gαo and anti-Gαi2 antibodies were particularly useful because they labeled the transduction cascade of V2R and V1R receptors, respectively. Both G proteins showed canonical immunohistochemical labeling in the vomeronasal organ and the AOB, consistent with the anterior-posterior zonation of the segregated model. The lectin Ulex europaeus agglutinin selectively labeled the anterior AOB, providing additional evidence for the segregation of vomeronasal information in the wallaby.Overall, the VNS of the Bennett’s wallaby shows a degree of differentiation and histochemical and neurochemical diversity comparable to species with greater VNS development, which does not support the existence of a third “intermediate” type of vomeronasal information processing.

scholarly journals Does a third intermediate model for the vomeronasal processing of information exist? Insights from the macropodid neuroanatomy

2021 ◽  
Author(s):  
Mateo V. Torres ◽  
Irene Ortiz-Leal ◽  
Paula R. Villamayor ◽  
Andrea Ferreiro ◽  
José Luis Rois ◽  
...  
Keyword(s):  
Vomeronasal Organ ◽  
Tammar Wallaby ◽  
Vomeronasal System ◽  
Mammal Species ◽  
Α Subunit ◽  
Intermediate Model ◽  
Ulex Europaeus ◽  
Vomeronasal Receptors ◽  
Segregated Model ◽  
Degree Of Differentiation

AbstractThe study of the α-subunit of Gi2 and Go proteins in the accessory olfactory bulb (AOB) was crucial for the identification of the two main families of vomeronasal receptors, V1R and V2R. Both families are expressed in the rodent and lagomorph AOBs, according to a segregated model characterized by topographical anteroposterior zonation. Many mammal species have suffered from the deterioration of the Gαo pathway and are categorized as belonging to the uniform model. This scenario has been complicated by characterization of the AOB in the tammar wallaby, Notamacropus eugenii, which appears to follow a third model of vomeronasal organization featuring exclusive Gαo protein expression, referred to as the intermediate model, which has not yet been replicated in any other species. Our morphofunctional study of the vomeronasal system (VNS) in Bennett’s wallaby, Notamacropus rufogriseus, provides further information regarding this third model of vomeronasal transduction. A comprehensive histological, lectin, and immunohistochemical study of the Bennett’s wallaby VNS was performed. Anti-Gαo and anti-Gαi2 antibodies were particularly useful because they labeled the transduction cascade of V2R and V1R receptors, respectively. Both G proteins showed canonical immunohistochemical labeling in the vomeronasal organ and the AOB, consistent with the anterior–posterior zonation of the segregated model. The lectin Ulex europaeus agglutinin selectively labeled the anterior AOB, providing additional evidence for the segregation of vomeronasal information in the wallaby. Overall, the VNS of the Bennett’s wallaby shows a degree of differentiation and histochemical and neurochemical diversity comparable to species with greater VNS development. The existence of the third intermediate type in vomeronasal information processing reported in Notamacropus eugenii is not supported by our lectin-histochemical and immunohistochemical findings in Notamacropus rufogriseus.

scholarly journals The olfactory system of the tammar wallaby is developed at birth and directs the neonate to its mother's pouch odours

Reproduction ◽  
2009 ◽  
Vol 138 (5) ◽  
pp. 849-857 ◽  
Author(s):  
Nanette Y Schneider ◽  
Terrence P Fletcher ◽  
Geoff Shaw ◽  
Marilyn B Renfree
Keyword(s):  
Olfactory System ◽  
Vomeronasal Organ ◽  
Tammar Wallaby ◽  
Main Olfactory Bulb ◽  
Macropus Eugenii ◽  
Main Olfactory Epithelium ◽  
Receptor Cells ◽  
Olfactory Systems ◽  
Main Olfactory System ◽  
Pouch Secretions

In kangaroos and wallabies at birth the highly altricial newborn young climbs unassisted from the urogenital opening to the teat. Negative geotropism is important for the initial climb to the pouch opening, but nothing is known of the signals that then direct the neonate downwards to the teat. Here we show that the newborn tammar wallaby (Macropus eugenii) has the olfactory apparatus to detect smell. Both the main olfactory system and vomeronasal organ (VNO) are developed at the time of birth. Receptor cells of the main olfactory epithelium immunopositive for Goα-protein project to the three layered main olfactory bulb (MOB). The receptor epithelium of the VNO contains G-protein immunopositive cells and has olfactory knob-like structures. The VNO is connected to an area between the two MOBs. Next, using a functional test, we show that neonates can respond to odours from their mother's pouch. When neonatal young are presented with a choice of a pouch-odour-soaked swab or a saline swab, they choose the swab with their mother's pouch secretions significantly more often (P<0.05) than the saline swab. We conclude that both olfactory systems are capable of receiving odour signals at birth, a function that must be a critical adaptation for the survival of an altricial marsupial neonate such as the tammar for its journey to the pouch.

scholarly journals Sex-linked and autosomal microsatellites provide new insights into island populations of the tammar wallaby

Heredity ◽  
2013 ◽  
Vol 112 (3) ◽  
pp. 333-342 ◽  
Author(s):  
A J MacDonald ◽  
N N FitzSimmons ◽  
B Chambers ◽  
M B Renfree ◽  
S D Sarre
Keyword(s):  
Tammar Wallaby ◽  
Island Populations ◽  
Autosomal Microsatellites

scholarly journals Host reproductive cycle influences the pouch microbiota of wild southern hairy-nosed wombats (Lasiorhinus latifrons)

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Sesilje Weiss ◽  
David Taggart ◽  
Ian Smith ◽  
Kristofer M. Helgen ◽  
Raphael Eisenhofer
Keyword(s):  
Microbial Community ◽  
Reproductive Cycle ◽  
Tammar Wallaby ◽  
The Body ◽  
Rrna Gene ◽  
Microbial Composition ◽  
Birth Canal ◽  
Gram Positive Bacteria ◽  
Rigorous Framework ◽  
The 16S Rrna Gene

Abstract Background Marsupials are born much earlier than placental mammals, with most crawling from the birth canal to the protective marsupium (pouch) to further their development. However, little is known about the microbiology of the pouch and how it changes throughout a marsupial’s reproductive cycle. Here, using stringent controls, we characterized the microbial composition of multiple body sites from 26 wild Southern Hairy-nosed Wombats (SHNWs), including pouch samples from animals at different reproductive stages. Results Using qPCR of the 16S rRNA gene we detected a microbial community in the SHNW pouch. We observed significant differences in microbial composition and diversity between the body sites tested, as well as between pouch samples from different reproductive stages. The pouches of reproductively active females had drastically lower microbial diversity (mean ASV richness 19 ± 8) compared to reproductively inactive females (mean ASV richness 941 ± 393) and were dominated by gram positive bacteria from the Actinobacteriota phylum (81.7–90.6%), with the dominant families classified as Brevibacteriaceae, Corynebacteriaceae, Microbacteriaceae, and Dietziaceae. Three of the five most abundant sequences identified in reproductively active pouches had closest matches to microbes previously isolated from tammar wallaby pouches. Conclusions This study represents the first contamination-controlled investigation into the marsupial pouch microbiota, and sets a rigorous framework for future pouch microbiota studies. Our results indicate that SHNW pouches contain communities of microorganisms that are substantially altered by the host reproductive cycle. We recommend further investigation into the roles that pouch microorganisms may play in marsupial reproductive health and joey survival.

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