degree of differentiation
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2022 ◽  
Vol 43 (2) ◽  
pp. 895-900
Author(s):  
Washington Luiz Assunção Pereira ◽  
◽  
Marcella Katheryne Marques Berna ◽  
Antônio Messias Costa ◽  
Aline Amaral Imbeloni ◽  
...  

Information on neoplasms in animals has increased over time, and these studies have helped in the management of reptiles that present tumors. There are similar incidences of neoplasms between homeothermic and ectothermic animals. Furthermore, there are usually more than one type of tumor present. The treatment of wild animals afflicted with cancer usually happens late, contributing to their low life expectancy. Thus, the present work aimed to describe an infrequent case of oral tumor in Boa constrictor. The tumor was observed in an adult female animal, raised in an exhibition area of the Zoo and Botanical Park of the Emílio Goeldi Museum, located in Belém, State of Pará, Brazil. Macroscopically, the mass presented morphologically with an irregular, multilobulated surface, color that varied from white to grey, hemorrhagic areas and its extension was 3.9 x 2.3 cm. The neoplasm was surgically removed, and the histopathological evaluation revealed an adenocarcinoma, with a papillary-like development pattern and a moderate degree of differentiation. The animal died three months after diagnosis due to starvation. The necropsy showed that there was tumor recrudescence and no metastases. Given the impossibility of surgical removal with a greater margin of safety, and adjuvant therapies, this condition favoured the resurgence of the neoplasm. This compromised the animal’s ability to feed and consequently lead to death. Malignant neoplasms in reptiles may have an unfavourable clinical evolution for the maintenance of life, requiring specific therapeutic care such as chemotherapy. Scientific contributions on tumors in these animals are essential for the medical treatment of wild animals, and the conservation of wild species.


2022 ◽  
Vol 11 ◽  
Author(s):  
Jianning Song ◽  
Hongzhong Zhou ◽  
Dayong Gu ◽  
Yong Xu

Hepatocellular carcinoma (HCC) is the most common primary malignant tumor of the liver. Although progress has been made in diagnosis and treatment, morbidity and mortality continue to rise. Chronic liver disease and liver cirrhosis are still the most important risk factors for liver cancer. Although there are many treatments, it can only be cured by orthotopic liver transplantation (OLT) or surgical resection. And the worse the degree of differentiation, the worse the prognosis of patients with liver cancer. Then it can be considered that restoring a better state of differentiation may improve the prognosis. The differentiation treatment of liver cancer is to reverse the dedifferentiation process of hepatocytes to liver cancer cells by means of drugs, improve the differentiation state of the tumor, and restore the normal liver characteristics, so as to improve the prognosis. Understanding the mechanism of dedifferentiation of liver cancer can provide ideas for drug design. Liver enrichment of transcription factors, imbalance of signal pathway and changes of tumor microenvironment can promote the occurrence and development of liver cancer, and restoring its normal level can inhibit the malignant behavior of tumor. At present, some drugs have been proved to be effective, but more clinical data are needed to support the effectiveness and reliability of drugs. The differentiation treatment of liver cancer is expected to become an important part of the treatment of liver cancer in the future.


2022 ◽  
pp. 10-15
Author(s):  
Waseem Al-Jameel ◽  
S. S. Al-Mahmood ◽  
A. M. Al-Saidya

Background and Aim: Squamous cell carcinoma (SCC) is the most common form of carcinoma in cattle. Histopathological grading systems have been utilized over several decades for estimating the malignancy of cattle SCCs. This study aimed to detect p53 and Mdm2 expression in different SCC cases in cattle and correlate their expression with the SCC histopathological grading. Materials and Methods: Cattle SCC cases were collected at the Veterinary Teaching Hospital in Nineveh. The SCC grading system categorized the cases histologically based on their differentiation grade into three groups: Well, moderately, and poorly differentiated. The SCC cases were subsequently verified for p53 and Mdm2 immunoexpression. Results: Fourteen of 16 examined cattle SCC samples tested positive for p53 expression. Moreover, 15 out of the 16 SCC samples tested positive for Mdm2 expression. The increased immunoreactivity of both p53 and Mdm2 was associated with a poor histological grading of the cattle SCC. There is a positive correlation between the nuclear expression of p53 and Mdm2, and the degree of differentiation and the number of mitotic figures in the examined cattle SCC samples. Conclusion: Our results demonstrate an increased p53 and Mdm2 expression in cattle SCC cases characterized by poor histopathological grading, thus suggesting an essential role of these molecules in the development of moderately and poorly differentiated SCC in cattle.


2022 ◽  
Vol 8 ◽  
Author(s):  
Pan Hao ◽  
Kai-yue Song ◽  
Si-qi Wang ◽  
Xiao-jun Huang ◽  
Da-wei Yao ◽  
...  

Tumorigenesis is associated with metabolic abnormalities and genomic instability. Microsatellite mutations, including microsatellite instability (MSI) and loss of heterozygosity (LOH), are associated with the functional impairment of some tumor-related genes. To investigate the role of MSI and LOH in sporadic breast tumors in canines, 22 tumors DNA samples and their adjacent normal tissues were evaluated using polyacrylamide gel electrophoresis and silver staining for 58 microsatellites. Quantitative real-time polymerase chain reaction, promoter methylation analysis and immunohistochemical staining were used to quantify gene expression. The results revealed that a total of 14 tumors (6 benign tumors and 8 breast cancers) exhibited instability as MSI-Low tumors. Most of the microsatellite loci possessed a single occurrence of mutations. The maximum number of MSI mutations on loci was observed in tumors with a lower degree of differentiation. Among the unstable markers, FH2060 (4/22), ABCC9tetra (4/22) and SCN11A (6/22) were high-frequency mutation sites, whereas FH2060 was a high-frequency LOH site (4/22). The ABCC9tetra locus was mutated only in cancerous tissue, although it was excluded by transcription. The corresponding genes and proteins were significantly downregulated in malignant tissues, particularly in tumors with MSI. Furthermore, the promoter methylation results of the adenosine triphosphate binding cassette subfamily C member 9 (ABCC9) showed that there was a high level of methylation in breast tissues, but only one case showed a significant elevation compared with the control. In conclusion, MSI-Low or MSI-Stable is characteristic of most sporadic mammary tumors. Genes associated with tumorigenesis are more likely to develop MSI. ABCC9 protein and transcription abnormalities may be associated with ABCC9tetra instability.


2021 ◽  
Vol 6 (6) ◽  
pp. 44-56
Author(s):  
L. I. Volos ◽  
◽  
A. P. Dudash

The purpose of the study was to assess the distribution of tumor-infiltrating T-, B-lymphocytes and natural killer cells in various molecular subtypes of invasive ductal breast cancer and to establish their relationship with the degree of tumor differentiation. Materials and methods. The basis of the scientific work was a complex morphological, including immunohistochemical study of 193 cases of invasive ductal breast cancer. General histological processing of the samples was carried out in accordance with the standard technique. Immunohistochemical studies for CD3, CD20, CD56, ER, PR, c-erbB2, Ki-67 were performed according to the manufacturer's protocol with the control of samples. The grade of malignancy was determined according to the modified scheme of P. Scarff, H. Bloom and W. Richardson. The presence, localization, and expression intensity of diagnostic and prognostic biomarkers CD3, CD20, and CD56-positive cells (T-, B-, and natural killer cells, respectively) were determined using the new bioimage analysis software QuPath. Results and discussion. The differences obtained in our study varied depending on the subpopulations of immune cells and their location in the tumor tissue. The density of T and B lymphocytes was higher within the tumor and in the invasive margin in the non-luminal phenotypes compared to the luminal A and B phenotypes. Compared to the density of T-lymphocytes, the B-cell infiltrate was significantly (p <0.01) less pronounced in tumors of both luminal and non-luminal phenotypes. The lowest density of natural killer cells among tumor-infiltrating lymphocytes was found within the tumor of luminal subtypes. The percentage of infiltrates of T and B cells both within the tumor focus and in the invasive margin, as well as natural killer cells in the invasive margin, was significantly lower with G1 and G2 than with the degree of differentiation G3 (p <0.01). As for intratumoral natural killer cells, differences were found only between the degree of differentiation of G2 and G3, and the differences between G1 and G2 were not statistically significant (p> 0.05). Qualitative and quantitative assessment of the intensity of expression of T-, B- and natural killer cells also demonstrated a different degree of expression of lymphocytes, depending on the molecular subtype and location of the infiltrate. The luminal subtype A was characterized by a significant predominance of mild expression (2+) CD3 both within the tumor and in the invasive margin of the tumor (p <0.05), while in the luminal B and Her2 + phenotypes, the intensity of CD3 expression prevailed at the 1+ level. Triple negative tumors were characterized by strong expression of (3+) CD3 within the tumor and in the invasive margin of the tumor in all cases studied. Conclusion. Thus, tumor-infiltrating lymphocytes in different molecular subtypes of invasive ductal breast cancer can be considered a prognostic biomarker. Our results indicate a relationship between lymphoid infiltrate and the degree of differentiation in invasive ductal breast cancer, especially in less favorable molecular subtypes


2021 ◽  
Vol 8 ◽  
Author(s):  
Hong Y. Choi ◽  
Isabelle Ruel ◽  
Shiwon Choi ◽  
Jacques Genest

The capacity of macrophages to dispose of cholesterol deposited in the atherosclerotic plaque depends on their ability to activate cholesterol efflux pathways. To develop athero-protective therapies aimed at promoting macrophage cholesterol efflux, cholesterol metabolism in THP-1 monocyte-derived macrophages has been extensively studied, but the intrinsic sensitivity of monocytes and the lack of a standardized procedure to differentiate THP-1 monocytes into macrophages have made it difficult to utilize THP-1 macrophages in the same or similar degree of differentiation across studies. The variability has resulted in lack of understanding of how the differentiation affects cholesterol metabolism, and here we review and investigate the effects of THP-1 differentiation on cholesterol efflux. The degree of THP-1 differentiation was inversely associated with ATP binding cassette A1 (ABCA1) transporter-mediated cholesterol efflux. The differentiation-associated decrease in ABCA1-mediated cholesterol efflux occurred despite an increase in ABCA1 expression. In contrast, DSC1 expression decreased during the differentiation. DSC1 is a negative regulator of the ABCA1-mediated efflux pathway and a DSC1-targeting agent, docetaxel showed high potency and efficacy in promoting ABCA1-mediated cholesterol efflux in THP-1 macrophages. These data suggest that pharmacological targeting of DSC1 may be more effective than increasing ABCA1 expression in promoting macrophage cholesterol efflux. In summary, the comparison of THP-1 macrophage subtypes in varying degrees of differentiation provided new insights into cholesterol metabolism in macrophages and allowed us to identify a viable target DSC1 for the promotion of cholesterol efflux in differentiated macrophages. Docetaxel and other pharmacological strategies targeting DSC1 may hold significant potential for reducing atherogenic cholesterol deposition.


2021 ◽  
Vol 8 ◽  
Author(s):  
Qiang Yan ◽  
Chenming Ni ◽  
Yingying Lin ◽  
Xu Sun ◽  
Zhenhua Shen ◽  
...  

Pancreatic cancer is one of the most lethal cancers and its prognosis is extremely poor. Clarification of molecular mechanisms and identification of prognostic biomarkers are urgently needed. Though we previously found that LGMN was involved in pancreatic carcinoma progression, the upstream regulation of LGMN remains unknown. We used reliable software to search for the potential transcription factors that may be related with LGMN transcription, we found that ELK1 could be a new regulator of LGMN transcription that binded directly to the LGMN promoter. Moreover, knocking down of ELK1 reduced pancreatic cancer cells proliferation, invasion and survival, while LGMN restored the malignancy of pancreatic cancer in vitro and in vivo. Overexpression of ELK1 further increased cancer cells proliferation, invasion and survival. Clinically, ELK1 and LGMN were positively correlated with clinical stage, degree of differentiation and Lymph node infiltration. ELK1 and LGMN were identified as independent prognostic factors for overall survival. The patients with low expression of ELK1/LGMN survived an average of 29.65 months, whereas those with high expression of ELK1/LGMN survived an average of 16.67 months. In conclusive, our results revealed a new mechanism by which ELK1 promoted the progression of pancreatic cancer via LGMN and conferred poor prognosis.


2021 ◽  
Vol 11 ◽  
Author(s):  
Wen-peng Huang ◽  
Li-ming Li ◽  
Jing Li ◽  
Jun-hui Yuan ◽  
Ping Hou ◽  
...  

PurposeHepatoid adenocarcinoma of the stomach (HAS) is a highly malignant and aggressive tumor. The purpose of this study was to describe the clinical, computed tomography (CT), and prognostic features of HAS to increase the awareness of this entity and determine its distinguishing features from non-HAS tumors.MethodsThe CT features and clinical data of 47 patients in our hospital with pathologically documented HAS were retrospectively analyzed, and the relevant differences between pure HAS (pHAS) and mixed HAS (mHAS) were determined. In addition, 141 patients with non-HAS tumors in the same T stage in the same period were selected as the control group. The data were compared between the two groups, and factors affecting the prognosis of HAS were analyzed. In addition, we included 9 patients with HAS and 27 patients with non-HAS tumors from another center for external validation.ResultsThe patients in the HAS group were predominantly men (n = 33), and the tumor location was mostly the cardia or fundus (n = 27). Between the HAS and non-HAS groups, there were observed differences in terms of: sex, serum alpha-fetoprotein (AFP), carbohydrate antigen (CA)-125, and CA-724 levels; longest tumor diameter; degree of differentiation; vascular invasion; N stage, M stage, and tumor-node-metastasis (TNM) stage; thickest tumor diameter; plain CT attenuation; arterial-phase CT attenuation; CT attenuation between the venous and arterial phases; enhancement modes; and degrees of enhancement (all P &lt; 0.05). In the data from another center for external validation, there were observed differences in terms of: age, degree of differentiation, vascular invasion, thickest tumor diameter, the ratio of arterial CT attenuation to CT attenuation of the abdominal aorta at the same level (RA), CT attenuation difference between the venous phase and arterial phase (HUv-a) (all P &lt; 0.05). The results of the multivariate analysis revealed that the independent factors for differentiation were serum AFP level (P = 0.001), M stage (P = 0.038), and tumor enhancement on CT (P = 0.014). Among patients in the HAS group, 72.34% had pHAS and 27.66% had mHAS. The thickest tumor diameter and the longest short diameter of the metastatic lymph nodes of the mHAS group were on average 6.39 cm and 1.45 cm, respectively, which were larger than those in the pHAS group. The median progression-free survival time was 18.25 months in the HAS group, which was shorter than that in the non-HAS group (72.96 months; P = 0.001). The median overall survival time in the HAS group was 24.80 months, which was shorter than that in the non-HAS group (67.96 months; P = 0.001). The factors affecting the prognosis of HAS were M stage (P = 0.001), overall TNM stage (P = 0.048), presence of vascular cancer emboli (P = 0.040), and pHAS type (P = 0.046). Multifactorial analysis revealed that M stage (P = 0.027) and pHAS type (P = 0.009) were independent risk factors affecting the prognosis of HAS.ConclusionAlthough HAS is a rare clinical entity, it should be considered in the differential diagnosis of gastric tumors. Patients with HAS often have advanced-stage disease at presentation and a worse prognosis than patients with non-HAS tumors. CT findings, combined with laboratory results, can support the diagnosis of HAS. However, the final diagnosis needs to be confirmed with a histopathologic examination. If the postoperative pathologic findings reveal the mHAS type, a rapid clinical intervention and a detailed follow-up with CT are essential.


2021 ◽  
Vol 63 (4) ◽  
pp. 308-316
Author(s):  
Sergiy Boiko

Abstract Changes that occur on the local level can explain the processes on the population level and, at the same time, are the driving force of species adaptation. This manuscript reports data about genetic diversities of the fungus Schizophyllum commune on the level of a local population. Objects of the study were dicarious cultures of S. commune collected from 38 basidiocarps grown on the territory of Holosiivskyi National Nature Park, Lysa Hora Regional Landscape Park and Feofaniya forest parcel (Ukraine). Results showed similarity of genetic variability of S. commune in different local populations. The heterozygote deficiency of some loci that was discovered might have resulted from new forms of allozymes that have not become widespread or due to small population sizes. The degree of differentiation of genes between local studied populations was moderate due to the high flow of genes. The absence of spatial structuration of genotypes is established, and the Mantel test showed a lack of interconnection between the genetic component and the geographical coordinates of the samples. It has been suggested that wind direction and terrain are the factors that influence the genetic structure of local populations.


Author(s):  
Elena Vyacheslavovna Litvikh

This article analyzes the intermezzo by J. Brahms&nbsp;Op. 119 No.3 for determining the nonclassical trends within the structure of musical fabric and form-making principles in this composition.&nbsp;The research employs the method of holistic analysis, which implies examination of the peculiarities of harmony, texture uniqueness, thematic processes, and formative patterns in the intermezzo by J. Brahms&nbsp;Op. 119 No.3; the elements of comparative analysis (juxtaposition of the form-making principles in this composition and classical works, as well as of interpretation of the reprise of the three-part form in the intermezzo by Brahms&nbsp;Op. 119 No.3 and Nocturne by Chopin&nbsp;Op. 48 №1).&nbsp;The scientific novelty lies in revealing the nonclassical trends in the music of J. Brahms, namely homogeneity of the sound fabric, low degree of differentiation of relief and background in the texture, absence of the common forms of sound and passive fragments in musical form.&nbsp;The author determines the conceptual proximity of Brahms' principles of working with musical material with the method of interfusion of the object and space that gained popularity in painting of the early&nbsp;XX&nbsp;century.&nbsp;The acquired results allow concluding that in the intermezzo Op. 119 No.3, J. Brahms implements a number of nonclassical principles of working with musical material, which anticipate the discoveries of the composers of the Second Viennese School (in particular, Webern).&nbsp;The presented materials can be valuable for further study of the compositions of J. Brahms, various theoretical aspects of music of the turn of the&nbsp;XIX &ndash;XX&nbsp;centuries, as well as performance and pedagogical practice.


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