Distribution of Spinal Cord Nerve Endings Containing Various Neurotransmitters on a Continuous Density Gradient

The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. One method involved loading digested tissue after creating a continuous density gradient (tissue after gradient [TAG]). The other method involved loading digested tissue before creating a continuous density gradient (tissue before gradient [TBG]). There were no significant differences between TAG and TBG in terms of the islet yield, rates of viability and purity, score, and in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of post-transplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.

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Identification of spinal afferent nerve endings in the colonic mucosa and submucosa that communicate directly with the spinal cord: The gut–brain axis

The Journal of Comparative Neurology ◽

10.1002/cne.24854 ◽

2020 ◽

Vol 528 (10) ◽

pp. 1742-1753 ◽

Cited By ~ 3

Author(s):

Nick J. Spencer ◽

Melinda A. Kyloh ◽

Lee Travis ◽

Kelsi N. Dodds

Keyword(s):

Spinal Cord ◽

Colonic Mucosa ◽

Afferent Nerve ◽

Nerve Endings ◽

Afferent Nerve Endings

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Influence de la cytochalasine A sur la composition de fractions subcellulaires d'hyphes en croissance du Mucor mucedo L. I. Composition du plasmalemme

Canadian Journal of Microbiology ◽

10.1139/m88-220 ◽

1988 ◽

Vol 34 (11) ◽

pp. 1256-1265 ◽

Cited By ~ 2

Author(s):

Abdou Ahmed Abd El Razak El Mougith ◽

Jean-Louis Fonvieille ◽

Robert Dargent ◽

Jacques Rami ◽

Jane-Marie Touzé-Soulet

Keyword(s):

Protein Content ◽

Density Gradient ◽

Density Gradient Centrifugation ◽

Gradient Centrifugation ◽

Sucrose Density Gradient ◽

Point Of View ◽

Continuous Density ◽

Mucor Mucedo ◽

Phospholipid Ratio ◽

Enzymatic Markers

The plasma membrane of young hyphae of Mucor mucedo L. growing in presence or absence of cytochalasine A was isolated by continuous density gradient centrifugation using Percoll at 10% or on discontinuous sucrose density gradient. Isolated membranes were characterized by enzymatic markers and cytochemical reactions, using electron microscopy. Lipid composition and protein content were determined. From the enzymatic point of view, the cytochalasine A induced a decrease (60%) in ATPase activity and with regard to the chemical composition of the membrane, a decrease in sterol content and in the sterol – phospholipid ratio as well as a decrease in protein content and an increase in the proportion of cysteine relative to other amino acids.

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