Arachidonic Acid and Other Unsaturated Fatty Acids Alter Membrane Potential in PC12 and Bovine Adrenal Chromaffin Cells

1993 ◽  
Vol 60 (1) ◽  
pp. 282-288 ◽  
Author(s):  
M. U. Ehrengruber ◽  
D. A. Deranleau ◽  
C. Kempf ◽  
P. Zahler ◽  
M. Lanzrein
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Membrane Potential ◽  
Chromaffin Cells ◽  
Unsaturated Fatty Acids ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Dietary unsaturated fatty acids differently affect catecholamine handling by adrenal chromaffin cells

2015 ◽  
Vol 26 (5) ◽  
pp. 563-570 ◽  
Author(s):  
Andreia Gomes ◽  
Gustavo Correia ◽  
Marisa Coelho ◽  
João Ricardo Araújo ◽  
Maria João Pinho ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Chromaffin Cells ◽  
Unsaturated Fatty Acids ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

scholarly journals Relationship between arachidonic acid release and Ca2+-dependent exocytosis in digitonin-permeabilized bovine adrenal chromaffin cells

1990 ◽  
Vol 271 (3) ◽  
pp. 571-574 ◽  
Author(s):  
A Morgan ◽  
R D Burgoyne
Keyword(s):  
Arachidonic Acid ◽  
Phospholipase A2 ◽  
Chromaffin Cells ◽  
Nordihydroguaiaretic Acid ◽  
Inhibitory Effect ◽  
Arachidonic Acid Release ◽  
Adrenal Chromaffin Cells ◽  
Protein Kinase C Inhibitor ◽  
Acid Release ◽  
Adrenal Chromaffin

The relationship between Ca2(+)-dependent arachidonic acid release and exocytosis from digitonin-permeabilized bovine adrenal chromaffin cells was investigated. The phospholipase A2 inhibitors mepacrine, nordihydroguaiaretic acid and indomethacin had no effect on either arachidonic acid release or secretion. The phospholipase A2 activator melittin had no effect on secretion. The specific diacylglycerol lipase inhibitor RG80267 had no effect on secretion, but decreased basal arachidonic acid release to such an extent that the level of arachidonic acid in treated cells in response to 10 microM-Ca2+ was equivalent to that of control cells in the absence of Ca2+. Staurosporine, a protein kinase C inhibitor, was found to abolish Ca2(+)-dependent arachidonic acid release completely, but had only a slight inhibitory effect on Ca2(+)-dependent secretion. It is concluded that arachidonic acid is not essential for Ca2(+)-dependent exocytosis in adrenal chromaffin cells.

scholarly journals Probes of transmembrane potentials in platelets: changes in cyanine dye fluorescence in response to aggregation stimuli

Blood ◽  
1978 ◽  
Vol 51 (4) ◽  
pp. 741-749
Author(s):  
WC Horne ◽  
ER Simons
Keyword(s):  
Membrane Potential ◽  
Platelet Aggregation ◽  
Chromaffin Cells ◽  
Transmembrane Potential ◽  
Human Platelets ◽  
Cyanine Dye ◽  
Adrenal Chromaffin Cells ◽  
Stimulus Response ◽  
Transmembrane Potentials ◽  
Adrenal Chromaffin

A noncovalent fluorescent probe that responded to changes in transmembrane potential was used to study the response of washed human platelets to aggregating agents. Concentration-dependent changes in the fluorescence were observed in response to ADP and to thrombin. No such changes were observed in response to collagen fibrils. Thus there was an indication that platelet membrane potential changed in response to aggregating stimuli, supporting the hypothesis that the mechanisms of platelet aggregation resembled the mechanisms of other systems that show stimulus-response coupling (e.g., muscle, adrenal chromaffin cells). The different responses to specific agents indicate that the agents may trigger platelet aggregation through different mechanisms.

scholarly journals Probes of transmembrane potentials in platelets: changes in cyanine dye fluorescence in response to aggregation stimuli

Blood ◽  
1978 ◽  
Vol 51 (4) ◽  
pp. 741-749 ◽  
Author(s):  
WC Horne ◽  
ER Simons
Keyword(s):  
Membrane Potential ◽  
Platelet Aggregation ◽  
Chromaffin Cells ◽  
Transmembrane Potential ◽  
Human Platelets ◽  
Cyanine Dye ◽  
Adrenal Chromaffin Cells ◽  
Stimulus Response ◽  
Transmembrane Potentials ◽  
Adrenal Chromaffin

Abstract A noncovalent fluorescent probe that responded to changes in transmembrane potential was used to study the response of washed human platelets to aggregating agents. Concentration-dependent changes in the fluorescence were observed in response to ADP and to thrombin. No such changes were observed in response to collagen fibrils. Thus there was an indication that platelet membrane potential changed in response to aggregating stimuli, supporting the hypothesis that the mechanisms of platelet aggregation resembled the mechanisms of other systems that show stimulus-response coupling (e.g., muscle, adrenal chromaffin cells). The different responses to specific agents indicate that the agents may trigger platelet aggregation through different mechanisms.

scholarly journals Stimulation of Ca2+-independent catecholamine secretion from digitonin-permeabilized bovine adrenal chromaffin cells by guanine nucleotide analogues. Relationship to arachidonate release

1990 ◽  
Vol 269 (2) ◽  
pp. 521-526 ◽  
Author(s):  
A Morgan ◽  
R D Burgoyne
Keyword(s):  
Arachidonic Acid ◽  
Chromaffin Cells ◽  
Binding Protein ◽  
Catecholamine Secretion ◽  
Arachidonic Acid Release ◽  
Adrenal Chromaffin Cells ◽  
Gtp Binding Protein ◽  
Gtp Binding ◽  
Acid Release ◽  
Adrenal Chromaffin

The effect of GTP analogues on catecholamine secretion and [3H]arachidonic acid release from digitonin-permeabilized adrenal chromaffin cells was examined. Several GTP analogues stimulated Ca2(+)-independent exocytosis, with the order of efficacy being XTP greater than ITP greater than guanosine 5′-[beta gamma-imido]triphosphate (p[NH]ppG) greater than guanosine 5′-[gamma-thio]triphosphate (GTP[S]). The stimulatory effect of the GTP analogues appeared to be due to activation of a conventional GTP-binding protein, as it was inhibited by guanosine 5′-[beta-thio]diphosphate (GDP[S]). In contrast, Ca2(+)-dependent exocytosis was only partially inhibited by high doses of GDP[S]. GTP did not stimulate Ca2(+)-independent exocytosis, but instead was found to inhibit secretion caused by micromolar Ca2+. Arachidonic acid (100 microM) also stimulated Ca2(+)-independent catecholamine secretion. Determination of the effect of GTP analogues on release of free [3H]arachidonic acid into the medium showed that it was stimulated by GTP[S] but inhibited by GTP, p[NH]ppG, ITP and XTP. The inhibition of [3H]arachidonic acid release by XTP was not prevented by GDP[S]. These results demonstrate that activation of a GTP-binding protein by certain GTP analogues can induce Ca2(+)-independent secretion in adrenal chromaffin cells and that the effect of GTP analogues on Ca2(+)-independent secretion can be dissociated from generation of arachidonic acid.

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