Identification and characterization of microsatellite markers derived from expressed sequence tags of the sea cucumber Stichopus japonicus

2007 ◽  
Vol 7 (6) ◽  
pp. 1057-1059 ◽  
Author(s):  
LIMEI CHEN ◽  
QI LI
Keyword(s):  
Microsatellite Markers ◽  
Expressed Sequence Tags ◽  
Sea Cucumber ◽  
Stichopus Japonicus ◽  
Identification And Characterization ◽  
Expressed Sequence

Computational identification and characterization of conserved miRNAs and their target genes in garlic (Allium sativum L.) expressed sequence tags

Gene ◽  
2014 ◽  
Vol 537 (2) ◽  
pp. 333-342 ◽  
Author(s):  
Debashis Panda ◽  
Budheswar Dehury ◽  
Jagajjit Sahu ◽  
Madhumita Barooah ◽  
Priyabrata Sen ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Allium Sativum ◽  
Target Genes ◽  
Computational Identification ◽  
Identification And Characterization ◽  
Expressed Sequence

scholarly journals Identification and Characterization of Ribosomal Protein S8 Gene of Jute

2010 ◽  
Vol 19 (2) ◽  
pp. 207-215
Author(s):  
Salim Ahmed ◽  
Md. Maksudul Alam ◽  
Md. Sazzadul Islam ◽  
MD. Shafiuddin ◽  
Md. Mosharrof Hossain Mondal ◽  
...  
Keyword(s):  
Ribosomal Protein ◽  
Expressed Sequence Tags ◽  
Genomic Library ◽  
Rt Pcr ◽  
Key Words Identification ◽  
Full Length Sequence ◽  
Identification And Characterization ◽  
Expressed Sequence ◽  
Common Laboratory

Identification and confirmation of ESTs (expressed sequence tags) corresponding to genomic clones is one of the most important steps in the identification of genes. In this regard, computational approaches such as ab initio and homology based searches using NCBI web portal, together with common laboratory approaches - PCR, RT-PCR and DNA sequencing were used to identify jute ESTs from a jute genomic library. Using degenerate primer-based gene walking from a computationally identified and experimentally verified jute EST, this study has led to the identification of the full length sequence of a jute gene, namely ribosomal protein S8. The sequence of this gene was found to be similar to ribosomal protein S8 gene of related species like Hibiscus macrophyllus, Gossypium hirsutum etc. and that of other species like Carica papaya, Arabidopsis thaliana etc. This gene was further characterized for determining its cellular location to the chloroplast.      Key words: Identification, Characterization, Ribosomal protein S8, Jute D.O.I. 10.3329/ptcb.v19i2.5438 Plant Tissue Cult. & Biotech. 19(2): 207-215, 2009 (December)

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