scholarly journals Identification and molecular characterization of a cyclic-di-GMP effector protein, PlzA (BB0733): additional evidence for the existence of a functional cyclic-di-GMP regulatory network in the Lyme disease spirochete,Borrelia burgdorferi

2010 ◽  
Vol 58 (2) ◽  
pp. 285-294 ◽  
Author(s):  
John C. Freedman ◽  
Elizabeth A. Rogers ◽  
Jessica L. Kostick ◽  
Hongming Zhang ◽  
Radha Iyer ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Molecular Characterization ◽  
Regulatory Network ◽  
Effector Protein

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

scholarly journals Characterization of outer membranes isolated from Borrelia burgdorferi, the Lyme disease spirochete.

1995 ◽  
Vol 63 (6) ◽  
pp. 2154-2163 ◽  
Author(s):  
J D Radolf ◽  
M S Goldberg ◽  
K Bourell ◽  
S I Baker ◽  
J D Jones ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Membranes

scholarly journals Molecular characterization of effector protein SAP54 in Bellis virescence phytoplasma (16SrIII-J)

2019 ◽  
Vol 44 (4) ◽  
pp. 392-397 ◽  
Author(s):  
Franco D. Fernández ◽  
Humberto J. Debat ◽  
Luis R. Conci
Keyword(s):  
Molecular Characterization ◽  
Effector Protein

scholarly journals Characterization of 5′-methylthioadenosine/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease

2020 ◽  
Vol 1864 (1) ◽  
pp. 129455 ◽  
Author(s):  
Kenneth A. Cornell ◽  
Reece J. Knippel ◽  
Gerald R. Cortright ◽  
Meghan Fonken ◽  
Christian Guerrero ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi

scholarly journals Molecular Evidence for a New Bacteriophage ofBorrelia burgdorferi

1999 ◽  
Vol 181 (23) ◽  
pp. 7308-7313 ◽  
Author(s):  
Christian H. Eggers ◽  
D. Scott Samuels
Keyword(s):  
Electron Microscopy ◽  
Borrelia Burgdorferi ◽  
Molecular Characterization ◽  
Linear Molecule ◽  
Molecular Evidence ◽  
Long Tail ◽  
Cell Free Supernatant ◽  
Dna Molecule ◽  
Low Levels

ABSTRACT We have recovered a DNase-protected, chloroform-resistant molecule of DNA from the cell-free supernatant of a Borrelia burgdorferi culture. The DNA is a 32-kb double-stranded linear molecule that is derived from the 32-kb circular plasmids (cp32s) of the B. burgdorferi genome. Electron microscopy of samples from which the 32-kb DNA molecule was purified revealed bacteriophage particles. The bacteriophage has a polyhedral head with a diameter of 55 nm and appears to have a simple 100-nm-long tail. The phage is produced constitutively at low levels from growing cultures of someB. burgdorferi strains and is inducible to higher levels with 10 μg of 1-methyl-3-nitroso-nitroguanidine (MNNG) ml−1. In addition, the prophage can be induced with MNNG from some Borrelia isolates that do not naturally produce phage. We have isolated and partially characterized the phage associated with B. burgdorferi CA-11.2A. To our knowledge, this is the first molecular characterization of a bacteriophage ofB. burgdorferi.

scholarly journals Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade, Serbia

2007 ◽  
Vol 38 (1) ◽  
pp. 14-16 ◽  
Author(s):  
Elizabeta S. Ristanovic ◽  
Kayoko Kitamura ◽  
Toshiyuki Masuzawa ◽  
Marija J. Milutinovic ◽  
Radovan M. Cekanac ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Molecular Characterization ◽  
Borrelia Burgdorferi Sensu Lato
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