scholarly journals Molecular Evidence for a New Bacteriophage ofBorrelia burgdorferi

1999 ◽  
Vol 181 (23) ◽  
pp. 7308-7313 ◽  
Author(s):  
Christian H. Eggers ◽  
D. Scott Samuels
Keyword(s):  
Electron Microscopy ◽  
Borrelia Burgdorferi ◽  
Molecular Characterization ◽  
Linear Molecule ◽  
Molecular Evidence ◽  
Long Tail ◽  
Cell Free Supernatant ◽  
Dna Molecule ◽  
Low Levels

ABSTRACT We have recovered a DNase-protected, chloroform-resistant molecule of DNA from the cell-free supernatant of a Borrelia burgdorferi culture. The DNA is a 32-kb double-stranded linear molecule that is derived from the 32-kb circular plasmids (cp32s) of the B. burgdorferi genome. Electron microscopy of samples from which the 32-kb DNA molecule was purified revealed bacteriophage particles. The bacteriophage has a polyhedral head with a diameter of 55 nm and appears to have a simple 100-nm-long tail. The phage is produced constitutively at low levels from growing cultures of someB. burgdorferi strains and is inducible to higher levels with 10 μg of 1-methyl-3-nitroso-nitroguanidine (MNNG) ml−1. In addition, the prophage can be induced with MNNG from some Borrelia isolates that do not naturally produce phage. We have isolated and partially characterized the phage associated with B. burgdorferi CA-11.2A. To our knowledge, this is the first molecular characterization of a bacteriophage ofB. burgdorferi.

scholarly journals Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade, Serbia

2007 ◽  
Vol 38 (1) ◽  
pp. 14-16 ◽  
Author(s):  
Elizabeta S. Ristanovic ◽  
Kayoko Kitamura ◽  
Toshiyuki Masuzawa ◽  
Marija J. Milutinovic ◽  
Radovan M. Cekanac ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Molecular Characterization ◽  
Borrelia Burgdorferi Sensu Lato

scholarly journals Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil

2005 ◽  
Vol 30 (4) ◽  
pp. 366-373 ◽  
Author(s):  
Álvaro M. R. Almeida ◽  
Junichi Sakai ◽  
Kaoru Hanada ◽  
Thalita G. Oliveira ◽  
Priscila Belintani ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Molecular Characterization ◽  
Sucrose Gradient ◽  
Open Reading Frame ◽  
Spherical Particles ◽  
Tobacco Streak Virus ◽  
Streak Virus ◽  
Reading Frame ◽  
Cp Gene

A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular characterization of TSV isolated from soybeans. It is proposed that this isolate be considered a strain of TSV named TSV-BR.

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