scholarly journals Development of a gene knockout system for Ralstonia eutropha H16 based on the broad-host-range vector expressing a mobile group II intron

2010 ◽  
pp. no-no ◽  
Author(s):  
Jong Myoung Park ◽  
Yu-Sin Jang ◽  
Tae Yong Kim ◽  
Sang Yup Lee
Keyword(s):  
Host Range ◽  
Ralstonia Eutropha ◽  
Gene Knockout ◽  
Group Ii Intron ◽  
Broad Host Range ◽  
Range Vector ◽  
Ralstonia Eutropha H16 ◽  
Group Ii ◽  
Broad Host Range Vector

scholarly journals Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron (“Targetron”) Expressed from a Broad-Host-Range Vector

2007 ◽  
Vol 73 (8) ◽  
pp. 2735-2743 ◽  
Author(s):  
Jun Yao ◽  
Alan M. Lambowitz
Keyword(s):  
Host Range ◽  
Gene Targeting ◽  
Inducible Promoter ◽  
Broad Host Range ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Efficient Gene ◽  
Range Vector ◽  
Group Ii ◽  
Broad Host Range Vector

ABSTRACT Mobile group II introns (“targetrons”) can be programmed for insertion into virtually any desired DNA target with high frequency and specificity. Here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon can be used for efficient gene targeting in a variety of gram-negative bacteria, including Escherichia coli, Pseudomonas aeruginosa, and Agrobacterium tumefaciens. Targetrons expressed from donor plasmids introduced by electroporation or conjugation yielded targeted disruptions at frequencies of 1 to 58% of screened colonies in the E. coli lacZ, P. aeruginosa pqsA and pqsH, and A. tumefaciens aopB and chvI genes. The development of this broad-host-range system for targetron expression should facilitate gene targeting in many bacteria.

A broad-host-range vector system for cloning and translational lacZ fusion analysis

Plasmid ◽  
1988 ◽  
Vol 19 (3) ◽  
pp. 175-188 ◽  
Author(s):  
Tsu-Ning Tai ◽  
Wendy A. Havelka ◽  
Samuel Kaplan
Keyword(s):  
Host Range ◽  
Lacz Fusion ◽  
Vector System ◽  
Broad Host Range ◽  
Range Vector ◽  
Fusion Analysis ◽  
Broad Host Range Vector

Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector

2002 ◽  
Vol 97 (3) ◽  
pp. 243-252 ◽  
Author(s):  
Humberto J.O Ramos ◽  
Lauren D.B Roncato-Maccari ◽  
Emanuel M Souza ◽  
Juliana R.L Soares-Ramos ◽  
Mariangela Hungria ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Range Vector ◽  
Broad Host Range Vector

Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression

Plasmid ◽  
1985 ◽  
Vol 13 (2) ◽  
pp. 149-153 ◽  
Author(s):  
Gary Ditta ◽  
Thomas Schmidhauser ◽  
Emanuel Yakobson ◽  
Peter Lu ◽  
Xiao-Wu Liang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Cloning ◽  
Host Range ◽  
Broad Host Range ◽  
Range Vector ◽  
Monitoring Gene Expression ◽  
Broad Host Range Vector

scholarly journals Maintenance of broad host range vector pKT230 in marine unicellular cyanobacteria

1992 ◽  
Vol 99 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Koji Sode ◽  
Masahiro Tatara ◽  
Shigeo Ogawa ◽  
Tadashi Matsunaga
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Range Vector ◽  
Broad Host Range Vector

Transformation of methylotrophic bacteria by electroporation

1997 ◽  
Vol 43 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Christian G. Gliesche
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Methylotrophic Bacteria ◽  
Methylobacterium Extorquens ◽  
Efficient System ◽  
Broad Host Range Plasmid ◽  
Phage Dna ◽  
Triparental Mating ◽  
Range Vector ◽  
Broad Host Range Vector

An efficient system for electroporation of the methylotrophic bacteria Hyphomicrobium facilis, Hyphomicrobium denitrificans, Methylobacillus glycogenes, Methylobacterium extorquens, and Methylophilus methylotrophus is described. It could be demonstrated that vectors based on the broad-host-range plasmid pBBR1 could be transferred into these strains. Plasmid pBBR1KAN (3.9 kb), a kanamycin-resistant derivative of pBBR1, was suitable for transformation experiments in these methylotrophic bacteria. Transformation efficiencies up to 104transformants/μg plasmid pBBR1KAN were obtained. The broad-host-range plasmid pLA2917 was transferred into Hyphomicrobium species by a triparental mating. However, this plasmid was integrated into the genome of Hyphomicrobium spp. Plasmids pLA2917, pKT231, pSUP2021, pRZ705, and phage DNA could not be transferred in Hyphomicrobium spp. by electroporation under the conditions applied.Key words: Hyphomicrobium, transformation, methylotrophic bacteria, plasmid pBBR1, broad-host-range vector.

Broad Host-Range Vector for Efficient Expression of Foreign Genes in Gram-Negative Bacteria

1991 ◽  
Vol 9 (5) ◽  
pp. 477-479 ◽  
Author(s):  
Shaukat H. Rangwala ◽  
Roy L. Fuchs ◽  
David J. Drahos ◽  
Peter O. Olins
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Range Vector ◽  
Efficient Expression ◽  
Foreign Genes ◽  
Broad Host Range Vector
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