Scanning electron microscopy of early microbial colonization of human enamel and root surfaces in vivo

1987 ◽  
Vol 95 (4) ◽  
pp. 287-296 ◽  
Author(s):  
BENTE NYVAD ◽  
OLE FEJERSKOV
Author(s):  
M.J.C. Hendrix ◽  
D.E. Morse

Atrial septal defects are considered the most common congenital cardiac anomaly occurring in humans. In studying the normal sequential development of the atrial septum, chick embryos of the White Leghorn strain were prepared for scanning electron microscopy and the results were then extrapolated to the human heart. One-hundred-eighty chick embryos from 2 to 21 days of age were removed from their shells and immersed in cold cacodylate-buffered aldehyde fixative . Twenty-four embryos through the first week post-hatching were perfused in vivo using cold cacodylate-buffered aldehyde fixative with procaine hydrochloride. The hearts were immediately dissected free and remained in the fixative a minimum of 2 hours. In most cases, the lateral atrial walls were removed during this period. The tissues were then dehydrated using a series of ascending grades of ethanol; final dehydration of the tissues was achieved via the critical point drying method followed by sputter-coating with goldpalladium.


PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57405 ◽  
Author(s):  
Bohumil Maco ◽  
Anthony Holtmaat ◽  
Marco Cantoni ◽  
Anna Kreshuk ◽  
Christoph N. Straehle ◽  
...  

2019 ◽  
Vol 25 (4) ◽  
pp. 2744-2750
Author(s):  
Ekaterina Karteva ◽  
◽  
Neshka Manchorova-Veleva ◽  
Zhelyazko Damyanov ◽  
Teodora Karteva ◽  
...  

1972 ◽  
Vol 39 (3) ◽  
pp. 247-296 ◽  
Author(s):  
Tadao Kirino ◽  
Takashi Ichijo ◽  
Masatoshi Goto ◽  
Takeshi Ono ◽  
Yukishige Kozawa ◽  
...  

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