scholarly journals Cytogenetic effects on in vivo bone-marrow cells of Mus musculus induced by a commercial 2,4,5-T ester product

Hereditas ◽  
2009 ◽  
Vol 85 (1) ◽  
pp. 123-134 ◽  
Author(s):  
LARS DäVRING ◽  
KERSTIN HULTGREN
Keyword(s):  
Bone Marrow ◽  
Mus Musculus ◽  
Bone Marrow Cells ◽  
Cytogenetic Effects ◽  
Ester Product ◽  
Marrow Cells

Environmental monitoring for genotoxicity: in vivo sister chromatid exchange in the house mouse (Mus musculus)

1985 ◽  
Vol 27 (3) ◽  
pp. 351-356 ◽  
Author(s):  
B. N. Nayak ◽  
M. L. Petras
Keyword(s):  
Bone Marrow ◽  
Sister Chromatid ◽  
Mus Musculus ◽  
Sister Chromatid Exchange ◽  
Bone Marrow Cells ◽  
Natural Populations ◽  
Wild Mice ◽  
Monitoring Procedure ◽  
Marrow Cells

Sister chromatid exchange (SCE) values were determined in bone marrow cells isolated from mouse (Mus musculus) femurs after injections of 5-bromo-2′-deoxyuridine (BrdU) and 5-fluorodeoxyuridine (FrdU). Male mice of C3H/J, C57BL/6J, and DBA/2 strains maintained in the laboratory gave mean SCE values of 3.42 ± 0.07, 3.62 ± 0.08, and 3.97 ± 0.13, respectively. Males obtained from natural populations of southwestern Ontario had a higher mean SCE value (6.02 ± 0.16), as did inbred males maintained in outdoor enclosures for at least 3 weeks (5.07 ± 0.22). Wild mice housed in the laboratory for 9 months or longer had SCE values similar to laboratory bred mice (3.46 ± 0.05). The SCE values in wild-caught mice were inversely proportional (r = −0.49) to the distance between the sites where these animals were collected and the nearest major industrial center. Based on these results, SCE analysis in mice is proposed as a possible first-line monitoring procedure for the detection of general changes in environmental genotoxicity.Key words: environmental genotoxicity, sister chromatid exchange, bone marrow cells, BrdU, FrdU, mouse.

Acute cytogenetic effects of potassium bromate on rat bone marrow cells in vivo

1988 ◽  
Vol 206 (4) ◽  
pp. 455-458 ◽  
Author(s):  
Kimiko Fujie ◽  
Hajime Shimazu ◽  
Mitsuko Matsuda ◽  
Taketoshi Sugiyama
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Potassium Bromate ◽  
Cytogenetic Effects ◽  
Rat Bone ◽  
Marrow Cells

scholarly journals Methylmercury-induced Cytogenetic Damage in Syrian Hamster Bone Marrow Cells

1984 ◽  
Vol 3 (4) ◽  
pp. 295-301
Author(s):  
J. B. Mailhes
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Single Injection ◽  
Cytogenetic Damage ◽  
Cytogenetic Effects ◽  
Response Increase ◽  
Environmental Toxin ◽  
Positive Controls ◽  
Marrow Cells

Methylmercury (MeHg) is an environmental toxin capable of interacting with chromatin and suspected of inducing chromosomal damage. In order to further evaluate the cytogenetic effects of MeHg in vivo, Syrian hamsters received a single injection of either 5, 10, 15, or 20 mg/kg MeHg 24 hours presacrifice and preparation of bone marrow cells for cytogenetic analysis. Negative and positive controls (0.25 mg/kg Trenimon) were also incorporated into the experimental design. The most prevalant type of cytogenetic damage observed was chromosome pulverization. A dose-response increase in the incidence of chromosomal aberrations following MeHg treatment was not observed. However, both MeHg- and Trenimon-treated hamsters had significantly higher proportions of cells with pulverized chromosomes than did controls. The observation that fewer cells with pulverized chromosomes were detected in the 15 and 20 mg/kg MeHg groups as compared with the 5 and 10 mg/kg MeHg groups was attributed to cellular toxicity. These data indicate that MeHg severely damages hamster bone marrow chromosomes, as demonstrated by chromosome pulverization.

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