Effects of chronic salt loading on plasma atrial natriuretic peptide (ANP) in the spontaneously hypertensive rat

1987 ◽  
Vol 129 (1) ◽  
pp. 67-72 ◽  
Author(s):  
K. GRADIN ◽  
J. HEDNER ◽  
T. HEDNER ◽  
A. C. TOWLE ◽  
A. PETTERSSON ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Spontaneously Hypertensive Rat ◽  
Salt Loading ◽  
Spontaneously Hypertensive ◽  
Plasma Atrial Natriuretic Peptide ◽  
Hypertensive Rat ◽  
Atrial Natriuretic

Immunoreactive atrial natriuretic peptide in neuronal and glial cells of spontaneously hypertensive rat brain

1990 ◽  
Vol 258 (1) ◽  
pp. C109-C114 ◽  
Author(s):  
M. K. Raizada ◽  
B. Kimura ◽  
M. I. Phillips
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Glial Cells ◽  
Spontaneously Hypertensive Rat ◽  
Culture Media ◽  
Spontaneously Hypertensive ◽  
Glial Cultures ◽  
The Media ◽  
Wistar Kyoto ◽  
Atrial Natriuretic

Neuronal and glial cultures from the hypothalamic-brain stem areas of 1-day-old normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SH) rat brains stained positively with atrial natriuretic peptide (ANP)-specific antibodies. The endogenous levels of the ANP immunoreactivity in WKY neuronal and glial cultures were 17.0 +/- 2.2 and 14.3 +/- 2.7 pg/mg, respectively. Comparable neuronal and glial cultures from SH rat brains contained a 48 to 70% decrease in the endogenous ANP immunoreactivity levels. Culture media from both brain cell types also contained ANP immunoreactivity, the levels of which are significantly higher than those found in the cells. However, similar to endogenous levels, the media levels of immunoreactive ANP in SH neuronal and glial cultures were significantly reduced compared with WKY brain cultures. These observations demonstrate that endogenous ANP-like immunoreactivity is found in neuronal and glial cells and is released into the media. The levels of peptide are reduced in cultures of SH compared with WKY cultures, suggesting a genetically controlled difference between the hypertensive and normotensive rat strains long before hypertension develops.

Sign in / Sign up

Export Citation Format

Share Document