Distribution of Calcitonin Gene?Related Peptide in the Central Nervous System of the Rat by Immunocytochemistry and in Situ Hybridization Histochemistry

1992 ◽  
Vol 657 (1 Calcitonin Ge) ◽  
pp. 455-457 ◽  
Author(s):  
ADELHEID KRESSE ◽  
DAVID M. JACOBOWITZ ◽  
GERHARD SKOFITSCH
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Calcitonin Gene Related Peptide ◽  
Hybridization Histochemistry ◽  
Related Peptide ◽  
In Situ Hybridization Histochemistry ◽  
Calcitonin Gene ◽  
The Central Nervous System

scholarly journals Non-radioactive detection of nerve growth factor receptor (NGFR) mRNA in rat brain using in situ hybridization histochemistry.

1991 ◽  
Vol 39 (2) ◽  
pp. 231-234 ◽  
Author(s):  
J E Springer ◽  
E Robbins ◽  
B J Gwag ◽  
M E Lewis ◽  
F Baldino
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Basal Forebrain ◽  
Growth Factor Receptor ◽  
Hybridization Histochemistry ◽  
In Situ Hybridization Histochemistry ◽  
The Central Nervous System

Radioactively labeled RNA probes in conjunction with in situ hybridization histochemistry have become a useful method for studying gene expression in the central nervous system. We used digoxigenin-labeled uridine triphosphate to synthesize cRNA probes for localization of nerve growth factor receptor (NGFR) mRNA in the rat basal forebrain. Detection of cells containing digoxigenin-labeled NGFR mRNA was accomplished using a digoxigenin antibody conjugated with alkaline phosphatase. NGFR mRNA-positive cells were distributed in three major cell groups in the basal forebrain: the medial septal nucleus, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. This technique provides a rapid and sensitive method for high-resolution detection of mRNA species in the central nervous system, as well as the potential for co-localization of two different mRNA species within individual cells.

Comparative immunohistochemical distribution of amylin-like and calcitonin gene related peptide like immunoreactivity in the rat central nervous system

1995 ◽  
Vol 73 (7) ◽  
pp. 945-956 ◽  
Author(s):  
Gerhard Skofitseh ◽  
Wolfgang Gubisch ◽  
Sunil J. Wimalawansa ◽  
David M. Jacobowitz
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cerebellar Nuclei ◽  
Calcitonin Gene Related Peptide ◽  
Polyclonal Antiserum ◽  
Related Peptide ◽  
Diagonal Band ◽  
Large Numbers ◽  
Calcitonin Gene ◽  
The Central Nervous System

Using the indirect immunofluorescence method with a polyclonal antiserum raised in rabbits and directed against amylin (AMY), we have investigated the distribution of AMY-like immunoreactivity (-ir) throughout the central nervous system of the rat. The widespread distribution of AMY-ir was much more abundant than that previously reported for calcitonin gene related peptide (CGRP) immunoreactivity. In most brain areas there was no overlap between AMY- and CGRP-ir cell body groupings, with the exception of the motor nuclei of the hindbrain and spinal cord, which were found to contain large numbers of AMY- and CGRP-immunoreactive cell bodies. Areas with a moderate to dense appearance of AMY-ir were the rhinencephalon, the nucleus of the diagonal band, the magnocellular, dorso- and ventro-medial and mammillary nuclei of the hypothalamus, the habenula, the compact part of the substantia nigra, the ruber and pontine nuclei, and the inferior olive and the cerebellar nuclei. The widespread immunohistochemical distribution of AMY-ir in the rat brain is in partial agreement with the distribution of AMY-binding sites.Key words: calcitonin gene related peptide, amylin, central nervous system, immunohistochemistry, rat.

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