Polarization and apoptosis of T cell subsets in idiopathic thrombocytopenic purpura

Abstract Chronic idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder in which the abnormality in cellular immunity has remained only vaguely defined. Previously we have shown that patients with ITP in its active phase have abnormal T cell subsets. We then examined the phenotypes of T and B lymphocytes in an additional 28 patients with ITP and 32 age- and sex-matched normal controls and compared the lymphocytes' capacity to respond to polyclonal T, T cell-dependent B, and B cell mitogens. Blastogenesis to optimal (5.0 micrograms/mL) and suboptimal (0.5 microgram/mL) concentrations of the polyclonal T cell mitogens were markedly depressed in patients compared with normal controls (P less than .0005). Similarly, a severe depression in response was noted with the polyclonal T cell-dependent B cell mitogen (P less than .000001). No difference was seen, however, with the polyclonal B cell mitogen. The proportions of pan-T and T helper/inducer lymphocytes were significantly depressed (P less than .005 and P less than .000005 respectively), and the T suppressor/cytotoxic lymphocytes increased (P less than .02) in patients relative to controls. But there was no difference in the proportion of B lymphocytes or in their functional response. The abnormal cellular immunity appears to be due to a defect in the T lymphocyte population without involvement of the B lymphocytes.

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Correction of abnormal T cell subsets by high-dose dexamethasone in patients with chronic idiopathic thrombocytopenic purpura

Immunology Letters ◽

10.1016/j.imlet.2013.08.006 ◽

2013 ◽

Vol 154 (1-2) ◽

pp. 42-48 ◽

Cited By ~ 23

Author(s):

Jianqin Li ◽

Zhaoyue Wang ◽

Shaoyan Hu ◽

Xiaojuan Zhao ◽

Lijuan Cao

Keyword(s):

T Cell ◽

Idiopathic Thrombocytopenic Purpura ◽

T Cell Subsets ◽

High Dose ◽

Chronic Idiopathic Thrombocytopenic Purpura ◽

Thrombocytopenic Purpura ◽

High Dose Dexamethasone

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Depressed functional and phenotypic properties of T but not B lymphocytes in idiopathic thrombocytopenic purpura

Blood ◽

10.1182/blood.v71.5.1455.bloodjournal7151455 ◽

1988 ◽

Vol 71 (5) ◽

pp. 1455-1460

Author(s):

R Mylvaganam ◽

RO Garcia ◽

YS Ahn ◽

PG Sprinz ◽

CI Kim ◽

...

Keyword(s):

T Cell ◽

B Cell ◽

B Lymphocytes ◽

Idiopathic Thrombocytopenic Purpura ◽

Cellular Immunity ◽

T Cell Subsets ◽

Phenotypic Properties ◽

Thrombocytopenic Purpura ◽

Cell Mitogen ◽

Normal Controls

Chronic idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder in which the abnormality in cellular immunity has remained only vaguely defined. Previously we have shown that patients with ITP in its active phase have abnormal T cell subsets. We then examined the phenotypes of T and B lymphocytes in an additional 28 patients with ITP and 32 age- and sex-matched normal controls and compared the lymphocytes' capacity to respond to polyclonal T, T cell-dependent B, and B cell mitogens. Blastogenesis to optimal (5.0 micrograms/mL) and suboptimal (0.5 microgram/mL) concentrations of the polyclonal T cell mitogens were markedly depressed in patients compared with normal controls (P less than .0005). Similarly, a severe depression in response was noted with the polyclonal T cell-dependent B cell mitogen (P less than .000001). No difference was seen, however, with the polyclonal B cell mitogen. The proportions of pan-T and T helper/inducer lymphocytes were significantly depressed (P less than .005 and P less than .000005 respectively), and the T suppressor/cytotoxic lymphocytes increased (P less than .02) in patients relative to controls. But there was no difference in the proportion of B lymphocytes or in their functional response. The abnormal cellular immunity appears to be due to a defect in the T lymphocyte population without involvement of the B lymphocytes.

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Differences in T cell subsets between men and women with idiopathic thrombocytopenic purpura

Blood ◽

10.1182/blood.v66.4.967.bloodjournal664967 ◽

1985 ◽

Vol 66 (4) ◽

pp. 967-972

Author(s):

R Mylvaganam ◽

YS Ahn ◽

WJ Harrington ◽

CI Kim ◽

HG Gratzner

Keyword(s):

T Cell ◽

Idiopathic Thrombocytopenic Purpura ◽

Autoimmune Disorder ◽

Active Phase ◽

T Cell Subsets ◽

T Helper ◽

Thrombocytopenic Purpura ◽

Men And Women ◽

Immune Imbalance ◽

Flow Cytofluorimetry

Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder, occurring predominantly in women. We studied by flow cytofluorimetry the T cell subsets in men and women with ITP and compared them with healthy sex-matched volunteers. In healthy controls, women were found to have higher proportions of T helper/inducer (Th/i) and lower T suppressor/cytotoxic (Ts/c) lymphocytes and consequently higher Th/i:Ts/c ratios than men. Accordingly, in clinical surveys, patients and controls should be matched for sex for proper comparisons. In patients with ITP in its active phase, an imbalance in T cell subsets was found in both sexes. The perturbation was more severe in women who had a marked decrease in number and proportion of Th/i lymphocytes and an increase in the proportion of Ts/c lymphocytes, whereas in men only, the proportion of Th/i lymphocytes was decreased. When patients with active disease were compared to those with ITP in remission, the decrease in Th/i subsets still persisted in both sexes but the Ts/c subset in women had returned to normal proportions. Therefore, the immune imbalance in ITP is more marked in women than men; imbalances in both Th/i and Ts/c are present in women while Ts/c appears not to be involved in men.

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Differences in T cell subsets between men and women with idiopathic thrombocytopenic purpura

Blood ◽

10.1182/blood.v66.4.967.967 ◽

1985 ◽

Vol 66 (4) ◽

pp. 967-972 ◽

Cited By ~ 27

Author(s):

R Mylvaganam ◽

YS Ahn ◽

WJ Harrington ◽

CI Kim ◽

HG Gratzner

Keyword(s):

T Cell ◽

Idiopathic Thrombocytopenic Purpura ◽

Autoimmune Disorder ◽

Active Phase ◽

T Cell Subsets ◽

T Helper ◽

Thrombocytopenic Purpura ◽

Men And Women ◽

Immune Imbalance ◽

Flow Cytofluorimetry

Abstract Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder, occurring predominantly in women. We studied by flow cytofluorimetry the T cell subsets in men and women with ITP and compared them with healthy sex-matched volunteers. In healthy controls, women were found to have higher proportions of T helper/inducer (Th/i) and lower T suppressor/cytotoxic (Ts/c) lymphocytes and consequently higher Th/i:Ts/c ratios than men. Accordingly, in clinical surveys, patients and controls should be matched for sex for proper comparisons. In patients with ITP in its active phase, an imbalance in T cell subsets was found in both sexes. The perturbation was more severe in women who had a marked decrease in number and proportion of Th/i lymphocytes and an increase in the proportion of Ts/c lymphocytes, whereas in men only, the proportion of Th/i lymphocytes was decreased. When patients with active disease were compared to those with ITP in remission, the decrease in Th/i subsets still persisted in both sexes but the Ts/c subset in women had returned to normal proportions. Therefore, the immune imbalance in ITP is more marked in women than men; imbalances in both Th/i and Ts/c are present in women while Ts/c appears not to be involved in men.

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