scholarly journals The enigmatic Crimean green lizard ( Lacerta viridis magnifica ) is extinct but not valid: Mitogenomics of a 120‐year‐old museum specimen reveals historical introduction

2019 ◽  
Vol 58 (1) ◽  
pp. 303-307
Author(s):  
Christian Kehlmaier ◽  
Oleksandr Zinenko ◽  
Uwe Fritz
2021 ◽  
pp. 1-12
Author(s):  
Robin-Tobias Jauss ◽  
Nadiné Solf ◽  
Sree Rohit Raj Kolora ◽  
Stefan Schaffer ◽  
Ronny Wolf ◽  
...  
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Author(s):  
Stella C. Yuan ◽  
Eric Malekos ◽  
Melissa T. R. Hawkins

AbstractThe use of museum specimens held in natural history repositories for population and conservation genetic research is increasing in tandem with the use of massively parallel sequencing technologies. Short Tandem Repeats (STRs), or microsatellite loci, are commonly used genetic markers in wildlife and population genetic studies. However, they traditionally suffered from a host of issues including length homoplasy, high costs, low throughput, and difficulties in reproducibility across laboratories. Massively parallel sequencing technologies can address these problems, but the incorporation of museum specimen derived DNA suffers from significant fragmentation and exogenous DNA contamination. Combatting these issues requires extra measures of stringency in the lab and during data analysis, yet there have not been any high-throughput sequencing studies evaluating microsatellite allelic dropout from museum specimen extracted DNA. In this study, we evaluate genotyping errors derived from mammalian museum skin DNA extracts for previously characterized microsatellites across PCR replicates utilizing high-throughput sequencing. We found it useful to classify samples based on DNA concentration, which determined the rate by which genotypes were accurately recovered. Longer microsatellites performed worse in all museum specimens. Allelic dropout rates across loci were dependent on sample quantity, with high concentration museum specimens performing as well and recovering quality metrics nearly as high as the frozen tissue sample. Based on our results, we provide a set of best practices for quality assurance and incorporation of reliable genotypes from museum specimens.


2016 ◽  
Vol 37 (2) ◽  
pp. 191-198 ◽  
Author(s):  
Dániel Kovács ◽  
István Kiss

Although Ablepharus kitaibelii and its subspecies are wide-spread, being distributed from the Carpathian Basin through the Balkans to Iraq, their habitat and environmental niche is poorly known. Ablepharus kitaibelii fitzingeri is almost entirely limited to the Carpathian Basin, and is amongst the most strictly protected and least known reptiles of Central and Eastern Europe. The main aim of our study was to determine habitat use preferences of different age groups of A. kitaibelii fitzingeri and Lacerta viridis. The occurrence of green lizard was determined by the abundance of refugia rather than by the naturalness of grasslands. The snake-eyed skink prefers semi-natural grasslands with abundant tussock-forming grass or sedge species, avoiding densely shrubby places. For the first time, we show that woodland mosaics lacking shrubs and temporary grasslands next to forest edges are important for the species. Microhabitat use by snake-eyed skink varies with age group; adults preferring shady edge zones rich in leaf litter and shadier grassland spots provided by woodland mosaics, whereas juveniles were found in natural, more open grasslands far from forest edges and in woodland mosaics with dense shrubby understory. Our results contribute to a better understanding of the ecological needs of A. kitaibelii fitzingeri. Our methodology could be adapted to other species and subspecies of Ablepharus. Based on our results, it is important to reconsider habitat management activities, which should not be limited to shrub control: the main goal should be the development of a diverse habitat structure.


1962 ◽  
Vol 25 (2) ◽  
pp. 233-237 ◽  
Author(s):  
J. G. PHILLIPS ◽  
I. CHESTER JONES ◽  
D. BELLAMY

SUMMARY Adrenal glands of lizards (Lacerta viridis L.) and snakes (Natrix natrix L.) were incubated in media containing tritiated progesterone. Aldosterone, corticosterone and an unknown steroid were produced. Added ACTH did not affect, though added amphenone markedly reduced, the rate of steroid production.


Mammalia ◽  
2019 ◽  
Vol 83 (5) ◽  
pp. 515-517
Author(s):  
Tauseef Hamid Dar ◽  
Manokaran Kamalakannan ◽  
Chinnadurai Venkatraman ◽  
Kailash Chandra

Abstract Hipposideros speoris is a small-sized leaf-nosed bat and was thought to be restricted to India and Sri Lanka. Based on a single museum specimen preserved in alcohol housed in the National Zoological Collections of the Zoological Survey of India, we report and confirm the presence of H. speoris for the first time in Pyay, Myanmar.


1982 ◽  
Vol 16 (1) ◽  
pp. 12-13 ◽  
Author(s):  
J. E. Cooper ◽  
S. Gschmeissner ◽  
P. E. Holt

1992 ◽  
Vol 70 (10) ◽  
pp. 1886-1896 ◽  
Author(s):  
Véronique Goosse ◽  
Vincent L. Bels

High-speed cinematography (100 frames/s) was used to allow quantitative analysis of the kinematic profiles of tongue and jaw displacements during chemosensory activities in the scleroglossan lizard Lacerta viridis. The types of tongue flicking were simple downward extensions (SDE), single oscillations (SOC), and submultiple oscillations (SMOC) of the tongue out of the mouth. The SMOC type involves a downward or upward movement of the tongue performed before a typical oscillation and it is therefore suggested that this is an intermediate category of flick between the typical SOC and MOC of lizards. Closing and opening of the mouth in SDE, SOC, and SMOC cycles may or may not be separated by a stationary stage during which the jaws are held open at a constant gape. The duration of this stationary interval increases from SDE to SMOC. Gape cycles do not show any division into slow and fast stages. The gape is produced largely by depression of the lower jaw; the upper jaw is slightly elevated by protrusion of the tongue. Patterns of correlation of kinematic variables depicting jaw and tongue movements differed between SDE, SOC, and SMOC. A principal component analysis shows that the three flick types overlap in a multivariate space constructed from the kinematic variables depicting jaw and tongue displacements. Overlap between SOC and SMOC categories is greater than that between SOC, SMOC, and SDE categories. The kinematic patterns of tongue displacement during SMOC in Lacerta viridis show similarities with those of MOC in other lizards and in snakes. Kinematically, the pattern of jaw and tongue displacements of Lacerta viridis during chemosensory activities shows similarities with those that occur during drinking and prey capture.


Author(s):  
Olga Tityuk ◽  
Volodymyr Grubiy ◽  
Yaroslav Stepanyuk ◽  
Oleksandr Yaryhin
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Описано приклад створення просторової реконструкції структур нюхового аналізатора (носової порожнини, вомероназального органа, нюхових нервів, нюхових цибулин) ембріона ящірки зеленої Lacerta viridis 34-ї стадії розвитку. Побудову просторової моделі проведено в програмі Amira 5.0.


2016 ◽  
Vol 47 (1) ◽  
pp. 17-43
Author(s):  
Petr Benda ◽  
Simon Engelberger

Abstract Seven historical bat specimens of four species (Rhinolophus ferrumequinum, R. mehelyi, Taphozous nudiventris, Myotis myotis), attributed to originate from the territory of the present-day Lebanon, are deposited and documented in the modern database of the mammal collection of the Natural History Museum, Vienna, Austria (Naturhistorisches Museum Wien). Two of these species (R. mehelyi, T. nudiventris) have never been reported for Lebanon in the existing literature and recent surveys have also failed to find them in this country. Since these bats were collected in the period 1824–1885, the history of the all respective specimens was evaluated in detail. The revision brought rather unexpected results. Only one specimen (R. ferrumequinum) was found to come (most probably) from Lebanon, being collected by W. Hemprich and Ch. Ehrenberg in 1824. In the remaining six specimens, the origin could not be defined, thus rendering the statement that they were collected in Lebanon insecure. This case demonstrates that careful checks of modern interpretations of historical records are necessary when examining past distributions of organisms.


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