IgE reactivity to shrimp allergens in infants and their cross-reactivity to house dust mite

ABSTRACTBackgroundThere are no set specific IgE (sIgE) to predict shrimp allergy as cross-reactivity with other arthropods play a role in shrimp sensitization.ObjectiveThis study identifies the allergens associated with shrimp allergy in house dust mite (HDM) and non-HDM sensitized patients.MethodsPatients with shrimp sensitization (positive skin prick test [SPT] and/or sIgE) with/without history of clinical reaction were recruited. Allergy was confirmed by oral food challenge (OFC) except for patients with history of anaphylaxis. Shrimp allergic (SA) and shrimp tolerant (ST) patients were further classified based on HDM sensitivity. The sIgE to shrimp, shrimp and HDM components were performed. Fisher’s exact test, Wilcoxon sum rank test and receiver operating characteristics analyses were done.ResultsOf 79 patients recruited, 12 SA (7 positive OFC and 5 with history of anaphylaxis), 18 ST and 10 non-shrimp sensitized controls (NC) were enrolled. In non-HDM sensitized patients, sIgE to shrimp (10.5 kUA/L, p=0.012) and Der p 10 (4.09 kUA/L, p=0.035) were higher in SA patients. Shrimp sIgE ≥3.55 kUA/L had 100% sensitivity and 85.71% specificity (ROC=0.94[0.81, 1.0]). Der p 10 sIgE ≥3.98 kUA/L had sensitivity of 80% and specificity of 100% (ROC=0.86[0.57, 1.0]). rPen a 1 ≥1.1 kUA/L had sensitivity of 80% and specificity of 85.7% (ROC=0.80[0.47,1.0]).ConclusionsIn non-HDM sensitized patients, shrimp sIgE ≥3.55 kUA/L and Der p 10 sIgE≥3.98 kUA/L give 100% sensitivity and specificity, respectively, to diagnose shrimp allergy. HDM sensitivity can influence sIgE levels to shrimp and shrimp/HDM components due to cross-reactivity.

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Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus

Journal of the Serbian Chemical Society ◽

10.2298/jsc0905513m ◽

2009 ◽

Vol 74 (5) ◽

pp. 513-522 ◽

Cited By ~ 1

Author(s):

Katarina Milovanovic ◽

Lidija Burazer ◽

Olga Vuckovic ◽

Marina Atanaskovic-Markovic ◽

Tanja Cirkovic-Velickovic ◽

...

Keyword(s):

Molecular Mass ◽

House Dust ◽

House Dust Mite ◽

Blot Analysis ◽

High Molecular Mass ◽

House Dust Mites ◽

House Dust Mite Allergy ◽

Ige Reactivity ◽

Mite Allergy ◽

Dust Mite

House dust mites (HDM) represent a major source of allergens, contributing to the increasing incidence of type I hypersensitivity disease worldwide. Over 30 different IgE-binding proteins from the HDM extract were detected. Although group 1 and 2 have been identified as major allergens, due to the safety and efficacy of allergy diagnosis and immunotherapy, there is a need to carefully evaluate the clinical relevance of other allergens present in the HDM extract. In regard to this, a high molecular mass allergen of about 68 kD was purified from the HDM extract using a combination of gel permeation chromatography and reversed-phase chromatography. The IgG and IgE reactivity of the purified protein were preserved during the purification process, as confirmed by Western blot analysis with polyclonal rabbit antibodies and dot blot analysis with a pool of sera from subjects with house dust mite allergy, respectively. In addition, the IgE reactivity was confirmed using ELISA testing with nine patient sera. The biological potency of the 68 kD allergen was confirmed by skin prick testing in five allergic subjects, suggesting that the high molecular mass allergen is a good candidate for component-resolved diagnosis of house dust mite allergy and eventual therapeutic treatment.

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Structure of the house dust mite allergen Der f 2: Implications for function and molecular basis of IgE cross-reactivity

FEBS Letters ◽

10.1016/j.febslet.2004.11.115 ◽

2005 ◽

Vol 579 (5) ◽

pp. 1208-1212 ◽

Cited By ~ 30

Author(s):

Birthe R. Johannessen ◽

Lars K. Skov ◽

Jette S. Kastrup ◽

Ole Kristensen ◽

Caroline Bolwig ◽

...

Keyword(s):

House Dust ◽

House Dust Mite ◽

Molecular Basis ◽

Cross Reactivity ◽

Mite Allergen ◽

House Dust Mite Allergen ◽

Dust Mite ◽

Dust Mite Allergen

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Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite

Clinical and Molecular Allergy ◽

10.1186/s12948-021-00142-z ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Su Duan ◽

Limin Zhao ◽

Yuling Zhang ◽

Nan Zhang ◽

Ming Zheng ◽

...

Keyword(s):

Allergic Rhinitis ◽

House Dust ◽

House Dust Mite ◽

Pollen Season ◽

Mononuclear Cells ◽

Cross Reactivity ◽

Amino Acid Sequences ◽

Th2 Response ◽

Dust Mite ◽

Elisa Inhibition

Abstract Background Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated. Methods Overall, 15,884 AR outpatients were assessed for clinical status. Amino acid sequences of mugwort were determined by mass spectrometry. Afterward, cross-reactivity between mugwort tropomyosin and Dermatophagoides pteronyssinus 10 (Der p10) was analysed by ELISA inhibition and basophil activation experiments. To compare immunologic responses eliciting by two different tropomyosins, peripheral blood mononuclear cells (PBMCs) of HDM-monosensitized patients were stimulated by mugwort, HDM, Der p10 and synthetic peptides representing mugwort tropomyosin respectively. Results Polysensitized HDM+AR patients were mainly sensitized to cat and mugwort, and the positive rate of monosensitized HDM+AR out-clinic patients was increased during the mugwort pollen season. Tropomyosin protein was able to find in mugwort. Synthetic tropomyosin peptide of mugwort activated basophils which were primed by HDM-specific IgE; ELISA inhibition experiment showed synthetic tropomyosin peptide of mugwort inhibited IgE binding to HDM tropomyosin, Der p10. Unlike HDM and Derp 10, mugwort and mugwort tropomyosin mainly induced IFN-γ and IL-17 release in PBMCs of monosensitized HDM+AR patients, but not IL-5. Conclusions Pan-allergen tropomyosin accounts for the cross-reactivity between mugwort and HDM, which reminds HDM+ patients to reduce mugwort exposure in mugwort pollen season in virtue of the tropomyosin induced mild inflammation.

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