Methyl‐β‐cyclodextrin and coronatine as new elicitors of tropane alkaloid biosynthesis in Atropa acuminata and Atropa belladonna hairy root cultures

2021 ◽  
Author(s):  
Farnoosh Fattahi ◽  
Abdolali Shojaeiyan ◽  
Javier Palazon ◽  
Elisabeth Moyano ◽  
Laura Torras‐Claveria
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Biosynthesis ◽  
Atropa Belladonna ◽  
Atropa Acuminata

Effects of acetylsalicylic acid and UV-B on gene expression and tropane alkaloid biosynthesis in hairy root cultures of Anisodus luridus

2014 ◽  
Vol 117 (3) ◽  
pp. 483-490 ◽  
Author(s):  
Baifu Qin ◽  
Lili Ma ◽  
Yaxiong Wang ◽  
Min Chen ◽  
Xiaozhong Lan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acetylsalicylic Acid ◽  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Biosynthesis

Tropane Alkaloid Production by the Establishment of Hairy Root Cultures of Brugmansia candida and Elicitation

2020 ◽  
pp. 123-132
Author(s):  
Alejandra B. Cardillo ◽  
María Perassolo ◽  
Juan M. Minoia ◽  
Julián Rodríguez Talou ◽  
Ana M. Giulietti
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Brugmansia Candida

scholarly journals Tropane alkaloid production and riboflavin excretion by Hyoscyamus niger L. hairy root cultures

2004 ◽  
Vol 53 (1) ◽  
pp. 14
Author(s):  
E Arak ◽  
K Pudersell ◽  
A Raal ◽  
R Vardja ◽  
T Vardja
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Hyoscyamus Niger

scholarly journals Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

2002 ◽  
Vol 43 (9) ◽  
pp. 1054-1058 ◽  
Author(s):  
Hiroyuki Fukami ◽  
Tomiko Asakura ◽  
Hiroshi Hirano ◽  
Keiko Abe ◽  
Koichiro Shimomura ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Atropa Belladonna ◽  
Carboxyl Methyltransferase

Establishment of hairy root cultures ofDatura stramonium. Characterization and stability of tropane alkaloid production during long periods of subculturing

1993 ◽  
Vol 33 (3) ◽  
pp. 321-329 ◽  
Author(s):  
I. E. Maldonado-Mendoza ◽  
T. Ayora-Talavera ◽  
V. M. Loyola-Vargas
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production

Enhanced production of tropane alkaloids by nitrate treatment in hairy root cultures of Atropa belladonna

2008 ◽  
Vol 136 ◽  
pp. S55 ◽  
Author(s):  
Najmeh Ahmadian Chashmi ◽  
Mozafar Sharifi ◽  
Farah Karimi ◽  
Hasan Rahnama
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Atropa Belladonna ◽  
Nitrate Treatment ◽  
Enhanced Production

Alkaloid production by hairy root cultures in Atropa belladonna

1986 ◽  
Vol 5 (4) ◽  
pp. 239-242 ◽  
Author(s):  
Hiroshi Kamada ◽  
Nobuyuki Okamura ◽  
Motoyoshi Satake ◽  
Hiroshi Harada ◽  
Koichiro Shimomura
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Atropa Belladonna

Induction of Tropane Alkaloid Formation in Transformed Root Cultures of Brugmansia suaveolens (Solanaceae)

2004 ◽  
Vol 59 (11-12) ◽  
pp. 863-867 ◽  
Author(s):  
Rawia Zayed ◽  
Michael Wink
Keyword(s):  
Salicylic Acid ◽  
Hairy Root ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Fresh Weight ◽  
Root Tips ◽  
Brugmansia Suaveolens ◽  
Set Up

Hairy root cultures of Brugmansia suaveolens were set up by infection of root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analysing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of tropane alkaloids, such as hyoscyamine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid in order to stimulate the biosynthesis of tropane alkaloids. Profile and amounts of tropane alkaloids were analysed using capillary GLC-MS. At least 18 different tropane alkaloids could be identified. Treatment of the cultures with 200 μᴍ methyljasmonate increased the alkaloid accumulation 25-fold up to a level of 1 mg/g fresh weight as compared to untreated controls. Quercetin enhanced the alkaloid production 10 fold (0.4 mg/g fresh weight) within 24 h. In contrast 100 μᴍ salicylic acid decreased alkaloids to a level of 1 μg/g fresh weight.

Sign in / Sign up

Export Citation Format

Share Document