Induction of Tropane Alkaloid Formation in Transformed Root Cultures of Brugmansia suaveolens (Solanaceae)

Hairy root cultures of Brugmansia suaveolens were set up by infection of root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analysing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of tropane alkaloids, such as hyoscyamine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid in order to stimulate the biosynthesis of tropane alkaloids. Profile and amounts of tropane alkaloids were analysed using capillary GLC-MS. At least 18 different tropane alkaloids could be identified. Treatment of the cultures with 200 μᴍ methyljasmonate increased the alkaloid accumulation 25-fold up to a level of 1 mg/g fresh weight as compared to untreated controls. Quercetin enhanced the alkaloid production 10 fold (0.4 mg/g fresh weight) within 24 h. In contrast 100 μᴍ salicylic acid decreased alkaloids to a level of 1 μg/g fresh weight.

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Induction of Pyridine Alkaloid Formation in Transformed Root Cultures of Nicotiana tabacum

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-11-1219 ◽

2009 ◽

Vol 64 (11-12) ◽

pp. 869-874 ◽

Cited By ~ 4

Author(s):

Rawia Zayed ◽

Michael Wink

Keyword(s):

Salicylic Acid ◽

Nicotiana Tabacum ◽

Hairy Root ◽

Inhibitory Effect ◽

Hairy Root Cultures ◽

Root Cultures ◽

Fresh Weight ◽

Root Tips ◽

Transformed Root Cultures ◽

Set Up

Hairy root cultures of Nicotiana tabacum were set up by excised root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analyzing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of pyridine alkaloids, mainly nicotine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid, in order to stimulate the biosynthesis of pyridine alkaloids. Profile and amounts of pyridine alkaloids were analyzed using capillary GLC-MS. Treatment of the cultures with methyljasmonate (50 μM) increased the alkaloid accumulation ca. 7-fold up to a level of 0.58 mg/g fresh weight as compared to untreated controls. Quercetin (200 μM) enhanced the alkaloid production ca. 4-fold (0.34 mg/g fresh weight) within 24 h. In contrast salicylic acid in all tested concentrations decreased the alkaloid level to 1 μg/g fresh weight. Also the inhibitory effect of salicylic acid on the elicitation effect of methyljasmonate and quercetin was investigated.

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The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida

Enzyme and Microbial Technology ◽

10.1016/s0141-0229(99)00137-4 ◽

2000 ◽

Vol 26 (2-4) ◽

pp. 252-258 ◽

Cited By ~ 145

Author(s):

Sandra I Pitta–Alvarez ◽

Tatiana C Spollansky ◽

Ana M Giulietti

Keyword(s):

Hairy Root ◽

Tropane Alkaloids ◽

Hairy Root Cultures ◽

Root Cultures ◽

Brugmansia Candida ◽

Abiotic Elicitors

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Tropane Alkaloid Production by the Establishment of Hairy Root Cultures of Brugmansia candida and Elicitation

Hairy Root Cultures Based Applications - Rhizosphere Biology ◽

10.1007/978-981-15-4055-4_9 ◽

2020 ◽

pp. 123-132

Author(s):

Alejandra B. Cardillo ◽

María Perassolo ◽

Juan M. Minoia ◽

Julián Rodríguez Talou ◽

Ana M. Giulietti

Keyword(s):

Hairy Root ◽

Tropane Alkaloid ◽

Hairy Root Cultures ◽

Root Cultures ◽

Alkaloid Production ◽

Brugmansia Candida

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Hairy Root Cultures and Plant Tropane Alkaloids: Production Matrix and Product in Biotechnological Perspective

Tropane Alkaloids ◽

10.1007/978-981-33-4535-5_5 ◽

2021 ◽

pp. 89-104

Author(s):

Shital K. Sharma ◽

Adarsh K. Agnihotri

Keyword(s):

Hairy Root ◽

Tropane Alkaloids ◽

Hairy Root Cultures ◽

Root Cultures

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Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48211891 ◽

2020 ◽

Vol 48 (2) ◽

pp. 839-848

Author(s):

Shuang ZHAO ◽

Hong TANG

Keyword(s):

Salicylic Acid ◽

Jasmonic Acid ◽

Methyl Jasmonate ◽

Hairy Root ◽

Hairy Roots ◽

Hairy Root Cultures ◽

Root Cultures ◽

Potent Inducer ◽

Valeriana Jatamansi ◽

Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

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