scholarly journals A TaqMan-based real-time PCR assay for specific detection of novel duck reovirus in China

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Shuai Zhang ◽  
Weihua Li ◽  
Xiaodong Liu ◽  
Xudong Li ◽  
Bin Gao ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay

Development of a taqman-based real-time PCR assay for the rapid and specific detection of novel duck- origin goose parvovirus

2017 ◽  
Vol 34 ◽  
pp. 56-58 ◽  
Author(s):  
Jianchang Wang ◽  
Jinfeng Wang ◽  
Yuan Cui ◽  
Huizhu Nan ◽  
Wanzhe Yuan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Goose Parvovirus

A New Real-Time PCR Assay for the Specific Detection of Salmonella spp. Targeting the bipA Gene

2008 ◽  
Vol 1 (4) ◽  
pp. 236-242 ◽  
Author(s):  
Laia Calvó ◽  
Asunción Martínez-Planells ◽  
Joana Pardos-Bosch ◽  
L. Jesús Garcia-Gil
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Salmonella Spp

Development of a TaqMan-based real-time PCR assay for the specific detection of porcine circovirus 3

2017 ◽  
Vol 248 ◽  
pp. 177-180 ◽  
Author(s):  
Jianchang Wang ◽  
Yongning Zhang ◽  
Jinfeng Wang ◽  
Libing Liu ◽  
Xiaoyu Pang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Porcine Circovirus ◽  
Specific Detection ◽  
Pcr Assay ◽  
Porcine Circovirus 3

Evaluation of a SYBR Green Real-Time PCR Assay for Specific Detection of Pasteurella multocida in Culture and Tissue Samples from Sheep

2020 ◽  
Author(s):  
Jyoti Kumar ◽  
G. G. Sonawane ◽  
Fateh Singh ◽  
S. Jegaveera Pandian ◽  
Rajiv Kumar
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pasteurella Multocida ◽  
Bacterial Species ◽  
Sybr Green ◽  
Economic Losses ◽  
Conventional Pcr ◽  
Specific Detection ◽  
Pcr Assay ◽  
Tissue Samples

Pasteurella multocida is one of the bacterial species involved in cases of ovine respiratory complex that has been implicated to cause significant economic losses in sheep production system worldwide. The present study was undertaken with the aim of evaluating a SYBR Green dye based real time PCR assay targeting KMT1 gene for the detection of P. multocida. The analytical specificity and sensitivity of the PCR primers were evaluated. The test showed ten-fold more sensitivity than conventional PCR and detected down to 275.5 fg/ µl of genomic DNA concentration, equivalent to 100 copies of KMT1 gene of P. multocida. The real-time PCR was found to be specific for KMT1 gene of P. multocida, as no cross reactivity was detected with a variety of known bacterial isolates. A total of 52 ovine lung tissue samples were screened for P. multocida, which showed improved level of detection as compared to conventional PCR. It is concluded that, this assay may be used as a valuable diagnostic tool for the rapid and specific detection of P. multocida. By virtue of its high throughput format and its ability to accurately identify as well as quantify the bacterial DNA, the method may be useful in large scale epidemiological studies and clarification of pathogenesis.

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