scholarly journals Serum creatine kinase isoenzymes and macroenzymes in dogs with different neurologic diseases

2017 ◽  
Vol 46 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Saverio Paltrinieri ◽  
Laura Pintore ◽  
Federica Balducci ◽  
Alessia Giordano ◽  
Annaluce Costabile ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Serum Creatine Kinase ◽  
Neurologic Diseases ◽  
Creatine Kinase Isoenzymes

Determination of serum creatine kinase isoenzymes in myocardial infarction

1972 ◽  
Vol 29 (6) ◽  
pp. 817-820 ◽  
Author(s):  
Aarne Konttinen ◽  
Hannu Somer
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Serum Creatine Kinase ◽  
Creatine Kinase Isoenzymes

Improved separation of creatine kinase isoenzymes by use of DEAE-Sepharose Cl-6B.

1980 ◽  
Vol 26 (5) ◽  
pp. 618-624 ◽  
Author(s):  
R J Bondar ◽  
D G Shevchik ◽  
M Y Hsu ◽  
M M Kohler
Keyword(s):  
Creatine Kinase ◽  
Reference Interval ◽  
Serum Creatine Kinase ◽  
Cross Contamination ◽  
Column System ◽  
Bed Height ◽  
Creatine Kinase Isoenzymes ◽  
Sepharose Column ◽  
Coefficients Of Variation ◽  
Chromatographic Procedure

Abstract We describe here a simple, rapid chromatographic procedure for quantitatively separating serum creatine kinase isoenzymes (EC 2.7.3.2), with diethylaminoethyl (DEAE)-Sepharose CL-6B as the anion-exchanger. We established the column bed height and the elution parameters by use of a simplex procedure. DEAE-Sepharose CL-6B equilibrated in tris(hydroxymethyl)aminomethane (50 mmol/L, pH 7.5, and containing 30 mmol of NaCl per liter) is packed into a miniature polystyrene column with bed dimensions of 0.7 x 1.5 cm. The DEAE-Sepharose column system was evaluated and compared with a DEAE-Sephadex A-50 column system. The results indicate that the Sepharose column yields MM, MB, and BB isoenzymes uniquely, without cross contamination. Coefficients of variation (CV’s) for 10 replicate column assays were 2.8, 5.9, and 15.2% for 569 U of MM per liter, 82.3 U of MB per liter, and 9.0 U of BB per liter, respectively. The serum sample was enriched with baboon heart extract. Day-to-day reproducibility for a serum control assayed on 10 days yielded CV’s of 4.6, 9.9, and 40.3% for 391, 45.3 and 1.9 U of isoenzymes MM, MB, and BB per liter, respectively. A reference interval for each isoenzymes was derived from data on 81 men and 63 women.

Presence of serum creatine kinase isoenzymes in liver failure with cerebral involvement

1977 ◽  
Vol 80 (2) ◽  
pp. 385-390 ◽  
Author(s):  
A. Lemberg ◽  
D.H. Werber ◽  
A. Gilardoni ◽  
H.J. Magrino
Keyword(s):  
Creatine Kinase ◽  
Liver Failure ◽  
Serum Creatine Kinase ◽  
Creatine Kinase Isoenzymes ◽  
Cerebral Involvement

Differential Diagnosis of Patients with Abnormal Serum Creatine Kinase Isoenzymes

1989 ◽  
Vol 9 (4) ◽  
pp. 627-642 ◽  
Author(s):  
John A. Lott ◽  
John M. Stang
Keyword(s):  
Creatine Kinase ◽  
Differential Diagnosis ◽  
Serum Creatine Kinase ◽  
Creatine Kinase Isoenzymes

scholarly journals Cord blood serum creatine kinase isoenzymes with placental dysfunction

1987 ◽  
Vol 15 (4) ◽  
pp. 350-354 ◽  
Author(s):  
Waldemar Niklinski ◽  
Zdzislaw Palynyczko ◽  
Michal Jozwik ◽  
Adam Sledziewski
Keyword(s):  
Creatine Kinase ◽  
Blood Serum ◽  
Cord Blood ◽  
Serum Creatine Kinase ◽  
Placental Dysfunction ◽  
Cord Blood Serum ◽  
Creatine Kinase Isoenzymes

Demonstration of serum creatine kinase isoenzymes by fluorescence technique

1972 ◽  
Vol 40 (1) ◽  
pp. 133-138 ◽  
Author(s):  
Hannu Somer ◽  
Aarne Konttinen
Keyword(s):  
Creatine Kinase ◽  
Serum Creatine Kinase ◽  
Fluorescence Technique ◽  
Creatine Kinase Isoenzymes

Improved separation of creatine kinase isoenzymes by use of DEAE-Sepharose Cl-6B.

1980 ◽  
Vol 26 (5) ◽  
pp. 618-624
Author(s):  
R J Bondar ◽  
D G Shevchik ◽  
M Y Hsu ◽  
M M Kohler
Keyword(s):  
Creatine Kinase ◽  
Reference Interval ◽  
Serum Creatine Kinase ◽  
Cross Contamination ◽  
Column System ◽  
Bed Height ◽  
Creatine Kinase Isoenzymes ◽  
Sepharose Column ◽  
Coefficients Of Variation ◽  
Chromatographic Procedure

Abstract We describe here a simple, rapid chromatographic procedure for quantitatively separating serum creatine kinase isoenzymes (EC 2.7.3.2), with diethylaminoethyl (DEAE)-Sepharose CL-6B as the anion-exchanger. We established the column bed height and the elution parameters by use of a simplex procedure. DEAE-Sepharose CL-6B equilibrated in tris(hydroxymethyl)aminomethane (50 mmol/L, pH 7.5, and containing 30 mmol of NaCl per liter) is packed into a miniature polystyrene column with bed dimensions of 0.7 x 1.5 cm. The DEAE-Sepharose column system was evaluated and compared with a DEAE-Sephadex A-50 column system. The results indicate that the Sepharose column yields MM, MB, and BB isoenzymes uniquely, without cross contamination. Coefficients of variation (CV’s) for 10 replicate column assays were 2.8, 5.9, and 15.2% for 569 U of MM per liter, 82.3 U of MB per liter, and 9.0 U of BB per liter, respectively. The serum sample was enriched with baboon heart extract. Day-to-day reproducibility for a serum control assayed on 10 days yielded CV’s of 4.6, 9.9, and 40.3% for 391, 45.3 and 1.9 U of isoenzymes MM, MB, and BB per liter, respectively. A reference interval for each isoenzymes was derived from data on 81 men and 63 women.

Serum Creatine Kinase Isoenzymes in Progressive Muscular Dystrophy

Enzyme ◽  
1978 ◽  
Vol 23 (4) ◽  
pp. 238-245 ◽  
Author(s):  
Elena Tzvetanova
Keyword(s):  
Creatine Kinase ◽  
Muscular Dystrophy ◽  
Serum Creatine Kinase ◽  
Progressive Muscular Dystrophy ◽  
Creatine Kinase Isoenzymes

Measurement of Brain-Specific Creatine Kinase Isoenzyme Activity in Serum

1975 ◽  
Vol 21 (11) ◽  
pp. 1663-1666 ◽  
Author(s):  
Daniel A Nealon ◽  
Arthur R Henderson
Keyword(s):  
Creatine Kinase ◽  
Serum Creatine Kinase ◽  
Brain Surgery ◽  
Chromatographic System ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Isoenzymes ◽  
Diethylaminoethyl Cellulose ◽  
Cellulose Column ◽  
Column Chromatographic

Abstract We further modified a diethylaminoethyl-cellulose column-chromatographic system [Clin. Chem. 21, 392 (1975)] so that it will separate all three serum creatine kinase isoenzymes. The modified system can be used to detect brain-specific creatine kinase isoenzyme in serum after brain surgery

A method allowing the quantitation of serum creatine kinase isoenzymes

1972 ◽  
Vol 36 (2) ◽  
pp. 531-536 ◽  
Author(s):  
Hannu Somer ◽  
Aarne Konttinen
Keyword(s):  
Creatine Kinase ◽  
Serum Creatine Kinase ◽  
Creatine Kinase Isoenzymes
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