Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

2020 ◽  
Author(s):  
Isis I. G. G. Taques ◽  
Maíra F. G. Koiyama ◽  
Amanda N. S. Campos ◽  
Jackeliny S. Costa ◽  
Kuang Hongyu ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Synthetic Peptides ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Ehrlichia Canis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Fluorescent Antibody

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

Sign in / Sign up

Export Citation Format

Share Document