A multicenter retrospective study on cryopreserved amniotic membrane transplantation for the treatment of complicated corneal ulcers in the dog

Objectives: To evaluate the efficacy and safety of amniotic membrane transplantation (AMT) for thetreatment of shield ulcer in vernal keratoconjunctivitis (VKC) with giant papillae.Material and Methods: This retrospective study was conducted in the Department of ophthalmologyHayatabad Medical Complex Peshawar from May 2009 to May 2010. In this study 30 patients of VKC withshield ulcer were evaluated for a period of one year. Both gender of 3 - 26 years age patients were included inthis study. Patients were enrolled and informed written consent was taken. Amniotic membraneimplantation was performed in thirty patients with grade 2 or grade 3 shield ulcers unresponsive toconventional medical treatment lasting an average of 14 weeks. Surgery was done under general anesthesiausing amniotic membrane as a therapeutic contact lens. Their record was maintained and all patients withthe mean 4 months follow-up were evaluated for about safety, efficacy, and side effect of the treatment.Results: Thirty eyes of thirty patients with grade 2 shield ulcer with opaque base) and grade 3 (plaque likelesions) not responding to steroid therapy with or without surgical debridement were enrolled. The ulcershealed with disintegration or retraction of the membrane in all patients within 2 weeks. A significantdecrease in symptoms and complete reepithelialization of the corneal ulcers were observed in all caseswithin the first 7 days. The eyes remain stable during a mean follow-up of 4 months, with no intraoperativeor postoperative complications. Absorption of amniotic membrane occurred with in 2 to 3 weeks timeleaving the underlying ulcer completely epithelized.Conclusion: Amniotic membrane transplantation in combination with debridement is a safe and effectivesurgical modality in the management of shield ulcers. Further studies are warranted to confirm the efficacyof amniotic membrane transplantation in the management of shield ulcer.

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Comparison of amniotic membrane transplantation with and without cultured limbal epithelium for persistent corneal ulcers

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2021.1924860 ◽

2021 ◽

Vol 35 (1) ◽

pp. 739-745

Author(s):

Rozaliya Hristova ◽

Yani Zdravkov ◽

Georgi Markov ◽

Davide Borroni ◽

Alexander Oscar ◽

...

Keyword(s):

Amniotic Membrane ◽

Amniotic Membrane Transplantation ◽

Corneal Ulcers ◽

Limbal Epithelium

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Role of multilayered amniotic membrane transplantation for the treatment of resistant corneal ulcers in North India

International Ophthalmology ◽

10.1007/s10792-013-9834-3 ◽

2013 ◽

Vol 34 (3) ◽

pp. 485-491 ◽

Cited By ~ 8

Author(s):

Shalini Mohan ◽

Ina Budhiraja ◽

Amit Saxena ◽

Perwez Khan ◽

S. K. Sachan

Keyword(s):

Amniotic Membrane ◽

North India ◽

Amniotic Membrane Transplantation ◽

Corneal Ulcers

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Sutureless “Contact Lens Sandwich” Technique for Amniotic Membrane Therapy of Central Corneal Ulcers

European Journal of Ophthalmology ◽

10.1177/11206721211049093 ◽

2021 ◽

pp. 112067212110490

Author(s):

Saverio V. Luccarelli ◽

Edoardo Villani ◽

Stefano Lucentini ◽

Francesco Bonsignore ◽

Matteo Sacchi ◽

...

Keyword(s):

Amniotic Membrane ◽

Contact Lens ◽

Low Cost ◽

Healing Time ◽

Amniotic Membrane Transplantation ◽

Recurrence Rates ◽

Sandwich Technique ◽

Corneal Ulcers ◽

Bandage Contact Lens ◽

Ring Complex

Purpose To describe a new technique for sutureless and glue-free amniotic membrane transplantation (AMT) and to investigate its effectiveness to treat corneal persistent epithelial defects (PEDs), compared to bandage contact lens (BCL) application alone. Methods We performed AMT with “contact lens sandwich technique” (CLS-AMT) in 8 consecutive patients with central/para-central (up to 4.00 mm from the geometrical centre) PED/ulceration and we retrospectively compared the results with 11 BCL procedures. Results The procedures were performed successfully with no complications. CLS-AMT showed significantly shorter healing time than BCL (24.0 ± 19.1 vs 42.9 ± 14.6 days; P < 0.05, Mann-Whitney test). Recurrence rates were 12% and 27% for CLS-AMT and BCL, respectively. Conclusion CLS-AMT technique, based on the suction effect due to the superposition of a bandage contact lens on the AM-ring complex, represents a quick, low cost, easy to perform and nearly non-invasive AMT technique. This approach is able to provide adequate fixation of AM, and it seems to be a safe and effective treatment for patients with PEDs.

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Efficacy of Amniotic Membrane Transplantation for the Treatment of Corneal Ulcers

Cornea ◽

10.1097/ico.0000000000002179 ◽

2020 ◽

Vol 39 (4) ◽

pp. 479-483 ◽

Cited By ~ 6

Author(s):

Kaspar Schuerch ◽

Andrea Baeriswyl ◽

Beatrice E. Frueh ◽

Christoph Tappeiner

Keyword(s):

Amniotic Membrane ◽

Amniotic Membrane Transplantation ◽

Corneal Ulcers

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Rate of epithelialisation and re-operations in corneal ulcers treated with amniotic membrane transplantation combined with botulinum toxin-induced ptosis

Graefe s Archive for Clinical and Experimental Ophthalmology ◽

10.1007/s00417-006-0493-1 ◽

2007 ◽

Vol 245 (7) ◽

pp. 955-964 ◽

Cited By ~ 2

Author(s):

Thomas Fuchsluger ◽

Emre Tuerkeli ◽

Henrike Westekemper ◽

Joachim Esser ◽

Klaus-Peter Steuhl ◽

...

Keyword(s):

Botulinum Toxin ◽

Amniotic Membrane ◽

Amniotic Membrane Transplantation ◽

Corneal Ulcers

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Active Pedicle Epithelial Flap Transposition Combined with Amniotic Membrane Transplantation for Treatment of Nonhealing Corneal Ulcers

Journal of Ophthalmology ◽

10.1155/2016/5742346 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7

Author(s):

Ting Zhang ◽

Yuexin Wang ◽

Yanni Jia ◽

Dongle Liu ◽

Suxia Li ◽

...

Keyword(s):

Confocal Microscopy ◽

Amniotic Membrane ◽

Ulcer Healing ◽

Laser Scanning ◽

Corneal Ulcer ◽

Anterior Segment ◽

Healing Time ◽

Stevens Johnson Syndrome ◽

Amniotic Membrane Transplantation ◽

Corneal Ulcers

Introduction. The objective was to evaluate the efficacy of active pedicle epithelial flap transposition combined with amniotic membrane transplantation (AMT) in treating nonhealing corneal ulcers.Material and Methods. Eleven patients (11 eyes) with nonhealing corneal ulcer who underwent the combined surgery were included. Postoperatively, ulcer healing time was detected by corneal fluorescein staining. Visual acuity, intraocular pressure, surgical complications, and recurrence were recorded. Corneal status was inspected by the laser scanning confocal microscopy and anterior segment optical coherence tomography (AS-OCT).Results. The primary diseases were herpes simplex keratitis (8 eyes), corneal graft ulcer (2 eyes), and Stevens-Johnson syndrome (1 eye). All epithelial flaps were intact following surgery, without shedding or displacement. Mean ulcer healing time was10.8±3.1days, with a healing rate of 91%. Vision significantly improved from 1.70 to 0.82 log MAR (P=0.001). A significant decrease in inflammatory cell infiltration and corneal stromal edema was revealed 2 months postoperatively by confocal microscopy and AS-OCT. Corneal ulcer recurred in 1 eye. None of the patients developed major complications.Conclusion. Active pedicle epithelial flap transposition combined with AMT is a simple and effective treatment for nonhealing corneal ulcers.

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