DNA barcoding and morphology reveal exceptional species diversity ofScoparia(Lepidoptera: Crambidae) from the Hailuogou Glacier area, China

The field of DNA barcoding is working towards generating a genetic system for the quick and accurate identification of eukaryotic species. For the more systematic minded, however, DNA barcoding offers a new approach towards screening and uniting large numbers of biological specimens in genetic groups as a first step towards assigning them to species and genera in an approach best termed “molecular-assisted alpha taxonomy”. This approach is particularly amenable in organisms with simple morphologies, a propensity for convergence, extensive phenotypic plasticity, and life histories with an alternation of heteromorphic generations. It is hard to imagine a group of organisms better defined by all of these traits than the marine macroalgae. In an effort to assess the utility of the DNA barcode (COI-5′) for testing the current concepts of biodiversity of marine macroalgae in Canada, a study to assess species diversity in the red algal family, Dumontiaceae, was initiated. Through this work I confirm the presence in Canadian waters of Dilsea californica (J. Agardh) Kuntze, Dilsea integra (Kjellman) Rosenvinge, and Neodilsea borealis (I.A. Abbott) Lindstrom of the Dilsea–Neodilsea complex, and Weeksia coccinea (Harvey) Lindstrom for the genus Weeksia . However, our work has uncovered two additional species of the former complex, Dilsea lindstromiae Saunders sp. nov. and Dilsea pygmaea (Setchell) Setchell, and an additional species of the latter, Weeksia reticulata Setchell, effectively doubling representation of these foliose dumontiacean genera in Canadian waters.

Download Full-text

Highlighting Astyanax Species Diversity through DNA Barcoding

PLoS ONE ◽

10.1371/journal.pone.0167203 ◽

2016 ◽

Vol 11 (12) ◽

pp. e0167203 ◽

Cited By ~ 30

Author(s):

Bruno César Rossini ◽

Carlos Alexandre Miranda Oliveira ◽

Filipe Augusto Gonçalves de Melo ◽

Vinicius de Araújo Bertaco ◽

Juan M. Díaz de Astarloa ◽

...

Keyword(s):

Species Diversity ◽

Dna Barcoding

Download Full-text

Reassessment of Species Diversity of the Subfamily Denticollinae (Coleoptera: Elateridae) through DNA Barcoding

PLoS ONE ◽

10.1371/journal.pone.0148602 ◽

2016 ◽

Vol 11 (2) ◽

pp. e0148602 ◽

Cited By ~ 13

Author(s):

Taeman Han ◽

Wonhoon Lee ◽

Seunghwan Lee ◽

In Gyun Park ◽

Haechul Park

Keyword(s):

Species Diversity ◽

Dna Barcoding

Download Full-text

Peer Review #1 of "DNA barcoding unveils skate (Chondrichthyes: Rajidae) species diversity in ‘ray’ products sold across Ireland and the UK (v0.1)"

10.7287/peerj.129v0.1/reviews/1 ◽

2013 ◽

Keyword(s):

Species Diversity ◽

Peer Review ◽

Dna Barcoding ◽

The Uk

Download Full-text

Illuminating species diversity of nudibranch in Indonesian coral reef ecosystem using molecular identification

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/944/1/012033 ◽

2021 ◽

Vol 944 (1) ◽

pp. 012033

Author(s):

I G W D Dharmawan ◽

D G Bengen ◽

I Setyobudiandi ◽

B Subhan ◽

I Verawati ◽

...

Keyword(s):

Species Diversity ◽

Genetic Distance ◽

Cytochrome Oxidase ◽

Dna Barcoding ◽

Morphological Characters ◽

Species Level ◽

Coi Gene ◽

Interspecific Genetic Distance ◽

The Difference ◽

Source Of Information

Abstract Nudibranch has high species diversity with complex morphological characters and is challenging to identify at the species level. The lack of knowledge about nudibranchs makes it difficult to identify conventionally using morphological characters. This study aims to identify nudibranchs at the species level using the DNA barcoding method from the mitochondrial cytochrome oxidase 1 (CO1) gen. The results of DNA barcoding using the Cytochrome Oxidase I (COI) gene showed 18 species of 51 samples analyzed. The phylogenetic tree reconstruction revealed 11 main clades belonging to 11 genera. The genetic distance between and within species clearly shows the difference between individuals. Interspecific genetic distance shows the lowest value between species was found between Chromodoris annae and Chromodoris magnifica is 0.075, and the largest genetic distance observed between species Glossodoris rufomarginata and Tritonidae sp is 0.354. This study shows molecular analysis can be used to identify nudibranch up to species level, which will be a source of information in knowing the distribution and the genetic distance.

Download Full-text

Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene

10.22541/au.161010667.72140058/v1 ◽

2021 ◽

Author(s):

Chuanjiang Zhou ◽

Mengxia Feng ◽

Yongtao Tang ◽

Changxing Yang ◽

Xiaolin Meng ◽

...

Keyword(s):

Species Diversity ◽

Cryptic Species ◽

Dna Barcoding ◽

Species Identification ◽

High Efficiency ◽

Morphological Characteristics ◽

Henan Province ◽

Coi Gene ◽

Morphological Identification ◽

Freshwater Shrimp

Freshwater shrimp is an extremely rich species group with a long and problematic taxonomic history, attributed to its wide distribution, numerous species and similar morphology. Shrimp diversity and species identification is utmost important for fisheries management. However, identification based on morphological characteristics is difficult and complex for a non-specialist to perform. The water system of Henan Province is relatively abundant, but there are few investigations of freshwater shrimps and no description of molecular features. The aim of this study was to uncover the species diversity and phylogenetic of freshwater shrimp in Henan province by combining morphological identification and molecular species delimitation (barcoding gene: COI gene). About 1,200 freshwater shrimp samples from 46 sampling sites were collected for preliminary traditional morphological identification, 222 samples of these were been further microscopic examination and molecular delimitation. Here we used tree based method (NJ, ML) and distance based method (ABGD, bPTP) mainly to define species, detect the cryptic species and assess the validity of the barcoding in molecular. Comprehensive morphological identification and molecular delimitation results, there were 9 effective species and more than one cryptic species of freshwater shrimp in the province and moreover all of them can be identified by DNA barcoding. The results of morphological identification and molecular identification show high consistency, which proves the high efficiency in freshwater shrimp species identification of the DNA barcoding and the presence of cryptic species.

Download Full-text