ISOLATION AND CHARACTERIZATION OF POLYHYDROXYALKANOATES (PHAS) PRODUCING BACTERIA FROM BRACKISH STREAM

2016 ◽  
Vol 78 (7) ◽  
Author(s):  
Nor Azimah Mohd Zain ◽  
Laila Muftah Ali Zargoun ◽  
Nur Fatihah Elias ◽  
Mohd Firdaus Abdul-Wahab ◽  
Mohd Suardi Suhaimi
Keyword(s):  
Nile Blue ◽  
Blue Staining ◽  
Pseudomonas Sp ◽  
Property A ◽  
Identification Procedure ◽  
Sudan Black B ◽  
Isolation And Characterization ◽  
Ft Ir ◽  
Lipid Granules

Polyhydroxyalkanoates (PHAs) are biopolymers which have similar characteristics with petrochemical plastics but a step better due to its biodegradable property. A total of 23 strains were isolated from two different brackish sources. In order to detect the PHAs granules, the PHAs producing bacteria were first screened with Sudan Black B staining. Twenty strains were observed with lipid granules and were subjected to further confirmation with Nile blue staining.  From the Nile blue staining, only 10 strains have the ability in producing PHAs and 2 were identified as strong PHAs producers. This study focuses on the 2 strains named S1 and L1. Further identification procedure was carried out and found that strain S1 and L1 belongs to Pseudomonas sp. L1 strain was found to be promising for PHAs production since it accumulated PHAs for about 88.3%. The PHAs produced by this strain was analyzed using Fourier Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) analysis and was identified as poly-3-hydroxybutyrate (P-3HB).

scholarly journals Isolation and characterization of -amylase from marine Pseudomonas sp. K6-28-040

2011 ◽  
Vol 10 (14) ◽  
pp. 2733-2740 ◽  
Author(s):  
Liu Jianguo ◽  
Zhang Zhiqiang ◽  
Zhu Hu ◽  
Dang Hongyue ◽  
Lu Jianren ◽  
...  
Keyword(s):  
Pseudomonas Sp ◽  
Isolation And Characterization

Isolation and characterization of Pseudomonas sp. NAF1 and its application in biodegradation of crude oil

2016 ◽  
Vol 75 (5) ◽  
Author(s):  
Abdullah M. El Mahdi ◽  
Hamidi Abdul Aziz ◽  
Salem S. Abu Amr ◽  
Nour Sh El-Gendy ◽  
Hussein N. Nassar
Keyword(s):  
Crude Oil ◽  
Pseudomonas Sp ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of hypeptin from Pseudomonas sp.

1989 ◽  
Vol 42 (10) ◽  
pp. 1460-1464 ◽  
Author(s):  
JUN''ICHI SHOJI ◽  
HIROSHI HINOO ◽  
TERUO HATTORI ◽  
KEIICHIRO HIROOKA ◽  
YASUO KIMURA ◽  
...  
Keyword(s):  
Pseudomonas Sp ◽  
Isolation And Characterization

Isolation and characterization of alkalotolerant Pseudomonas sp. strain ISTDF1 for degradation of dibenzofuran

2010 ◽  
Vol 38 (4) ◽  
pp. 503-511 ◽  
Author(s):  
Prashant Kumar Jaiswal ◽  
Shweta Kohli ◽  
Madhuban Gopal ◽  
Indu Shekhar Thakur
Keyword(s):  
Pseudomonas Sp ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of spliceostatin B, a new analogue of FR901464, from Pseudomonas sp. No. 2663

2013 ◽  
Vol 66 (9) ◽  
pp. 555-558 ◽  
Author(s):  
Xiangyang Liu ◽  
Sreya Biswas ◽  
Gong-Li Tang ◽  
Yi-Qiang Cheng
Keyword(s):  
Pseudomonas Sp ◽  
Isolation And Characterization

Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim

2010 ◽  
Vol 21 (6) ◽  
pp. 939-946 ◽  
Author(s):  
Hua Fang ◽  
Yiqi Wang ◽  
Chunming Gao ◽  
Hu Yan ◽  
Bin Dong ◽  
...  
Keyword(s):  
Pseudomonas Sp ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Cellulose Nanofibers fromGigantochloa scortechiniias a Reinforcement Material

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Chaturbhuj K. Saurabh ◽  
Asniza Mustapha ◽  
M. Mohd. Masri ◽  
A. F. Owolabi ◽  
M. I. Syakir ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cellulose Nanofibers ◽  
X Ray Diffraction ◽  
X Ray ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Reinforcement Material ◽  
Bamboo Fibers ◽  
Ft Ir

Cellulose nanofibers (CNF) were isolated fromGigantochloa scortechiniibamboo fibers using sulphuric acid hydrolysis. This method was compared with pulping and bleaching process for bamboo fiber. Scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and thermogravimetric analysis were used to determine the properties of CNF. Structural analysis by FT-IR showed that lignin and hemicelluloses were effectively removed from pulp, bleached fibers, and CNF. It was found that CNF exhibited uniform and smooth morphological structures, with fiber diameter ranges from 5 to 10 nm. The percentage of crystallinity was significantly increased from raw fibers to cellulose nanofibers, microfibrillated, along with significant improvement in thermal stability. Further, obtained CNF were used as reinforcement material in epoxy based nanocomposites where tensile strength, flexural strength, and modulus of nanocomposites improved with the addition of CNF loading concentration ranges from 0 to 0.7%.

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