Megalin mediates transepithelial albumin clearance from the alveolar space of intact rabbit lungs

SummaryThe radioactive adenosine 3′,5′-monophosphate (cyclic AMP) level derived from 8-14C adenine in intact rabbit platelets decreased in the presence of mitochondrial inhibitor (potassium cyanide) or uncoupler (sodium azide), and markedly increased by the addition of NaF, monoiodoacetic acid (MIA), or 2-deoxy-D-glucose. The stimulative effect of the glycolytic inhibitors was distinctly enhanced by the simultaneous addition of sodium succinate. MIA did neither directly stimulate the adenyl cyclase activity nor inhibit the phosphodiesterase activity. These results suggest that cyclic AMP synthesis in platelets is closely linked to mitochondrial oxidative phosphorylation.

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Effect of Cold on the Isolated and Intact Rabbit Heart

Journal of Applied Physiology ◽

10.1152/jappl.1958.13.3.422 ◽

1958 ◽

Vol 13 (3) ◽

pp. 422-424 ◽

Cited By ~ 7

Author(s):

Benjamin G. Covino ◽

W. R. Beavers

Keyword(s):

Rabbit Heart ◽

Intact Rabbit

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Molecular mapping of pulmonary endothelial membrane glycoproteins of the intact rabbit lung

The FASEB Journal ◽

10.1096/fasebj.4.12.2168327 ◽

1990 ◽

Vol 4 (12) ◽

pp. 3040-3048 ◽

Cited By ~ 6

Author(s):

Marilyn P. Merker ◽

William W. Carley ◽

G. N. Gillis

Keyword(s):

Molecular Mapping ◽

Membrane Glycoproteins ◽

Rabbit Lung ◽

Intact Rabbit

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Effects of fetal corticosteroid treatments on postnatal surfactant function in preterm lambs

Journal of Applied Physiology ◽

10.1152/jappl.1995.79.3.846 ◽

1995 ◽

Vol 79 (3) ◽

pp. 846-851 ◽

Cited By ~ 14

Author(s):

T. Ueda ◽

M. Ikegami ◽

D. Polk ◽

K. Mizuno ◽

A. Jobe

Keyword(s):

Surface Area ◽

Dynamic Compliance ◽

Alveolar Space ◽

Prenatal Treatment ◽

Ultrasound Guided ◽

Functional Characteristics ◽

Arterial Pco2 ◽

Alveolar Surfactant ◽

Pool Sizes

Effects of prenatal corticosteroid on the properties of surfactant have not previously been evaluated. A single ultrasound-guided fetal injection with 0.5 mg/kg betamethasone 48 h before delivery of preterm lambs at 134- to 135-days gestation improved oxygenation, lowered the ventilatory pressures required to maintain arterial PCO2 between 30 and 40 Torr and decreased the protein leak of albumin from the intravascular to the alveolar space. This dose of glucocorticoid did not alter surfactant-saturated phosphatidylcholine pool sizes in the airspaces of preterm lambs. However, the treatment changed the characteristics of the surfactant recovered from the ventilated preterm lambs. The in vitro conversion from heavy to light subtype surfactant decreased from 59% for the saline-treated lambs to 37% for the corticosteroid-treated lambs after 180 min of surface area cycling (P < 0.02). Surfactant from the corticosteroid-treated lambs also increased the dynamic compliance of preterm surfactant-deficient rabbits more than did surfactant from the saline-treated lambs (P < 0.05). Prenatal treatment of preterm lambs with betamethasone improved the functional characteristics of surfactant without significant effects on the alveolar surfactant pool sizes.

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Electronic TV-pupillometric device and its adaptation for studying drug penetration into the intact rabbit eye

Experimental Eye Research ◽

10.1016/0014-4835(77)90322-0 ◽

1977 ◽

Vol 24 (1) ◽

pp. 102

Author(s):

A. Palkama ◽

K. Kalliomäki ◽

S-E. Masar

Keyword(s):

Drug Penetration ◽

Rabbit Eye ◽

Intact Rabbit

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Direct Effect of Halothane and Isoflurane on the Function of the Sarcoplasmic Reticulum in Intact Rabbit Atria

Anesthesiology ◽

10.1097/00000542-199004000-00019 ◽

1990 ◽

Vol 72 (4) ◽

pp. 694-698 ◽

Cited By ~ 20

Author(s):

Hirochika Komai ◽

Ben F. Rusy

Keyword(s):

Sarcoplasmic Reticulum ◽

Direct Effect ◽

Intact Rabbit ◽

Rabbit Atria

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Evidence for glycine, GABAA, and GABAB receptors on rabbit OFF-alpha ganglion cells

Visual Neuroscience ◽

10.1017/s0952523803203072 ◽

2003 ◽

Vol 20 (3) ◽

pp. 285-296 ◽

Cited By ~ 12

Author(s):

THOMAS C. ROTOLO ◽

RAMON F. DACHEUX

Keyword(s):

Ganglion Cells ◽

Gabab Receptors ◽

Direct Inhibition ◽

Light Responses ◽

Retinal Surface ◽

Excitatory Response ◽

Sulforhodamine B ◽

Intact Rabbit ◽

Specific Receptors ◽

Whole Cell Recordings

Inhibitory synaptic transmission via GABA and glycine receptors plays a crucial role in shaping the excitatory response of neurons in the retina. Whole-cell recordings were obtained from ganglion cells in the intact rabbit eyecup preparation to correlate GABA- and glycine-activated currents with the presence of their specific receptors on morphologically identified α ganglion cells. Alpha ganglion cells were chosen based upon their large somata when viewing the retinal surface, and responses to light and dark spots were used to identify OFF-alpha ganglion cells. Light responses were abolished by superfusion of Ringer's containing cobalt to synaptically isolate the cell by blocking all Ca2+-mediated transmitter release. Pressure pulses of GABA and glycine were delivered to an area that encompassed the dendritic field while receptor antagonists were applied through superfusion to characterize the direct inhibition onto the ganglion cell. Physiological results indicated that OFF-α cells did not have any GABAC receptor-activated currents, but did express currents mediated by ionotropic GABAA receptors and metabotropic GABAB receptors that were blocked by their specific antagonists bicuculline and CGP55845, respectively. The amplitudes of strychnine-sensitive glycine-activated currents were always larger than the currents elicited by GABA. Confocal optical sections of physiologically identified, sulforhodamine B-stained cells displayed the localization of glycine and GABAA receptor subunit labeling dispersed over the stained dendrites.

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