Registration accuracy between whole slide images and glass slides in eeDAP workflow

2018 ◽  
Author(s):  
Qi Gong ◽  
Benjamin P. Berman ◽  
Marios A. Gavrielides ◽  
Brandon D. Gallas
Keyword(s):  
Registration Accuracy ◽  
Glass Slides ◽  
Whole Slide Images

scholarly journals A Digital Pathology Solution to Resolve the Tissue Floater Conundrum

2020 ◽  
Author(s):  
Liron Pantanowitz ◽  
Pamela Michelow ◽  
Scott Hazelhurst ◽  
Shivam Kalra ◽  
Charles Choi ◽  
...  
Keyword(s):  
Digital Pathology ◽  
Primary Diagnosis ◽  
Image Search ◽  
Molecular Tests ◽  
Tissue Identification ◽  
Digital Database ◽  
Glass Slides ◽  
Search Tool ◽  
Hematoxylin And Eosin ◽  
Whole Slide Images

Context.— Pathologists may encounter extraneous pieces of tissue (tissue floaters) on glass slides because of specimen cross-contamination. Troubleshooting this problem, including performing molecular tests for tissue identification if available, is time consuming and often does not satisfactorily resolve the problem. Objective.— To demonstrate the feasibility of using an image search tool to resolve the tissue floater conundrum. Design.— A glass slide was produced containing 2 separate hematoxylin and eosin (H&E)-stained tissue floaters. This fabricated slide was digitized along with the 2 slides containing the original tumors used to create these floaters. These slides were then embedded into a dataset of 2325 whole slide images comprising a wide variety of H&E stained diagnostic entities. Digital slides were broken up into patches and the patch features converted into barcodes for indexing and easy retrieval. A deep learning-based image search tool was employed to extract features from patches via barcodes, hence enabling image matching to each tissue floater. Results.— There was a very high likelihood of finding a correct tumor match for the queried tissue floater when searching the digital database. Search results repeatedly yielded a correct match within the top 3 retrieved images. The retrieval accuracy improved when greater proportions of the floater were selected. The time to run a search was completed within several milliseconds. Conclusions.— Using an image search tool offers pathologists an additional method to rapidly resolve the tissue floater conundrum, especially for those laboratories that have transitioned to going fully digital for primary diagnosis.

Proof of Concept: The Use of Whole-Slide Images (WSI) for Peer Review of Tissues on Routine Regulatory Toxicology Studies

2021 ◽  
pp. 019262332098325
Author(s):  
Alys E. Bradley ◽  
Maurice G. Cary ◽  
Kaori Isobe ◽  
Stuart Naylor ◽  
Stephen Drew
Keyword(s):  
Peer Review ◽  
Proof Of Concept ◽  
Regulatory Toxicology ◽  
Glass Slides ◽  
Microscopic Findings ◽  
Slight Discrepancy ◽  
Whole Slide Images ◽  
Diagnostic Discrepancies

This Proof of Concept (POC) study was to assess whether assessment of whole slide images (WSI) of the 2 target tissues for a contemporaneous peer review can elicit concordant results to the findings generated by the Study Pathologist from the glass slides. Well-focused WSI of liver and spleen from 4 groups of mice, that had previously been diagnosed to be the target tissues by an experienced veterinary toxicologic pathologist examining glass slides, were independently reviewed by 3 veterinary pathologists with varying experience in assessment of WSIs. Diagnostic discrepancies were then reviewed by an experienced adjudicating pathologist. Assessment of microscopic findings using WSI showed concordance with the glass slides, with only slight discrepancy in severity grades noted. None of the lesions recorded by the Study pathologist were “missed” and no lesions were added by the pathologists evaluating WSIs, thus demonstrating equivalence of the WSI to glass slides for this study.

scholarly journals Digital Whole Slide Imaging Compared With Light Microscopy for Primary Diagnosis in Surgical Pathology

2020 ◽  
Vol 144 (10) ◽  
pp. 1245-1253 ◽  
Author(s):  
Alexander D. Borowsky ◽  
Eric F. Glassy ◽  
William Dean Wallace ◽  
Nathash S. Kallichanda ◽  
Cynthia A. Behling ◽  
...  
Keyword(s):  
Digital Pathology ◽  
Diagnostic Category ◽  
Primary Diagnosis ◽  
Current Standard ◽  
Diagnostic Categories ◽  
Standard Glass ◽  
Specific Subset ◽  
Glass Slides ◽  
Major Discrepancy ◽  
Whole Slide Images

Context.— The adoption of digital capture of pathology slides as whole slide images (WSI) for educational and research applications has proven utility. Objective.— To compare pathologists' primary diagnoses derived from WSI versus the standard microscope. Because WSIs differ in format and method of observation compared with the current standard glass slide microscopy, this study is critical to potential clinical adoption of digital pathology. Design.— The study enrolled a total of 2045 cases enriched for more difficult diagnostic categories and represented as 5849 slides were curated and provided for diagnosis by a team of 19 reading pathologists separately as WSI or as glass slides viewed by light microscope. Cases were reviewed by each pathologist in both modalities in randomized order with a minimum 31-day washout between modality reads for each case. Each diagnosis was compared with the original clinical reference diagnosis by an independent central adjudication review. Results.— The overall major discrepancy rates were 3.64% for WSI review and 3.20% for manual slide review diagnosis methods, a difference of 0.44% (95% CI, −0.15 to 1.03). The time to review a case averaged 5.20 minutes for WSI and 4.95 minutes for glass slides. There was no specific subset of diagnostic category that showed higher rates of modality-specific discrepancy, though some categories showed greater discrepancy than others in both modalities. Conclusions.— WSIs are noninferior to traditional glass slides for primary diagnosis in anatomic pathology.

Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan

2015 ◽  
Vol 59 (3) ◽  
pp. 278-283 ◽  
Author(s):  
Jen-Fan Hang ◽  
Wen-Yih Liang ◽  
Chih-Yi Hsu ◽  
Chiung-Ru Lai
Keyword(s):  
Educational Program ◽  
Large Scale ◽  
Imaging System ◽  
Whole Slide Imaging ◽  
Easy Method ◽  
Web Based ◽  
Glass Slides ◽  
Promising Solution ◽  
First Time ◽  
Whole Slide Images

Objective: In a peer comparison educational program, transferring glass slides between laboratories and collecting responses are time- and cost-consuming. Integrating a web-based whole-slide imaging (WSI) system and online questionnaires may serve as a promising solution. Study Design: Five gynecologic Papanicolaou-stained smears and 5 nongynecologic slides were selected. The 10 whole-slide images were acquired by a Leica SCN-400 system and released via an Aperio eSlide Manager. Online questionnaires generated by Google Forms with access to the 10 whole-slide images were released to all the practitioners in Taiwan by e-mail. After closing the program, an online posttest feedback survey was conducted. Results: A total of 302 participants joined the gynecologic test, and 291 joined the nongynecologic test. The correct interpretation rates were 81.8-93.7% in the former and 28.5-93.1% in the latter. In the posttest feedback survey, there were 63.2% of the participants reporting first-time WSI experience, and 97.9% of them said they would like to participate in a similar program again. Conclusion: Integrating a web-based WSI system and online questionnaires is an easy method to access nationwide practitioners. Participants can make interpretations using WSI even without prior experience. The model is valuable for those who want to initiate a large-scale cytopathology peer comparison educational program.

Cytotechnologists Accurately Pre-screen Prostate Cores on Traditional Glass Slides and Digital Whole-Slide Images

2015 ◽  
Vol 4 (6) ◽  
pp. S8-S9
Author(s):  
Louis Vaickus ◽  
Kristine Cornejo ◽  
Brenda Sweeney ◽  
Mary Rego ◽  
Heather Smith ◽  
...  
Keyword(s):  
Glass Slides ◽  
Whole Slide Images

scholarly journals Validation of Digital Microscopy Compared With Light Microscopy for the Diagnosis of Canine Cutaneous Tumors

2018 ◽  
Vol 55 (4) ◽  
pp. 490-500 ◽  
Author(s):  
Christof A. Bertram ◽  
Corinne Gurtner ◽  
Martina Dettwiler ◽  
Olivia Kershaw ◽  
Kristina Dietert ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Diagnostic Performance ◽  
Cell Tumor ◽  
Digital Microscopy ◽  
Round Cell ◽  
Round Cell Tumor ◽  
Glass Slides ◽  
Diagnostic Time ◽  
Tumor Types ◽  
Whole Slide Images

Integration of new technologies, such as digital microscopy, into a highly standardized laboratory routine requires the validation of its performance in terms of reliability, specificity, and sensitivity. However, a validation study of digital microscopy is currently lacking in veterinary pathology. The aim of the current study was to validate the usability of digital microscopy in terms of diagnostic accuracy, speed, and confidence for diagnosing and differentiating common canine cutaneous tumor types and to compare it to classical light microscopy. Therefore, 80 histologic sections including 17 different skin tumor types were examined twice as glass slides and twice as digital whole-slide images by 6 pathologists with different levels of experience at 4 time points. Comparison of both methods found digital microscopy to be noninferior for differentiating individual tumor types within the category epithelial and mesenchymal tumors, but diagnostic concordance was slightly lower for differentiating individual round cell tumor types by digital microscopy. In addition, digital microscopy was associated with significantly shorter diagnostic time, but diagnostic confidence was lower and technical quality was considered inferior for whole-slide images compared with glass slides. Of note, diagnostic performance for whole-slide images scanned at 200× magnification was noninferior in diagnostic performance for slides scanned at 400×. In conclusion, digital microscopy differs only minimally from light microscopy in few aspects of diagnostic performance and overall appears adequate for the diagnosis of individual canine cutaneous tumors with minor limitations for differentiating individual round cell tumor types and grading of mast cell tumors.

scholarly journals Breast cancer prognostic factors in the digital era: Comparison of Nottingham grade using whole slide images and glass slides

2019 ◽  
Vol 10 (1) ◽  
pp. 11 ◽  
Author(s):  
JoannG Elmore ◽  
TaraM Davidson ◽  
MaraH Rendi ◽  
PaulD Frederick ◽  
Tracy Onega ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Digital Era ◽  
Glass Slides ◽  
Nottingham Grade ◽  
Whole Slide Images

scholarly journals Whole Slide Images and Digital Media in Pathology Education, Testing, and Practice: The Oklahoma Experience

2012 ◽  
Vol 35 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Kar-Ming Fung ◽  
Lewis A. Hassell ◽  
Michael L. Talbert ◽  
Allan F. Wiechmann ◽  
Brad E. Chaser ◽  
...  
Keyword(s):  
Digital Media ◽  
Modular Architecture ◽  
Still Images ◽  
Glass Slides ◽  
Hands On ◽  
Traditional Lectures ◽  
Logistic Issue ◽  
Pathology Training ◽  
Whole Slide Images ◽  
Pathology Education

Examination of glass slides is of paramount importance in pathology training. Until the introduction of digitized whole slide images that could be accessed through computer networks, the sharing of pathology slides was a major logistic issue in pathology education and practice. With the help of whole slide images, our department has developed several online pathology education websites. Based on a modular architecture, this program provides online access to whole slide images, still images, case studies, quizzes and didactic text at different levels. Together with traditional lectures and hands-on experiences, it forms the back bone of our histology and pathology education system for residents and medical students. The use of digitized whole slide images has a.lso greatly improved the communication between clinicians and pathologist in our institute.

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