Enhancing near-ultraviolet circular dichroism and chirality-sorting optical forces using dielectric metasurfaces (Conference Presentation)

D uring ribonuclease S (RN ase S) refolding, two peptide fragm ents recognize each other, and bind to g eth er to form a refolding in term ediate which slowly converts to th e nativ e state. We have characterized this refolding interm ediate using absorbance, circular dichroism (CD), and nuclear m agnetic resonance (NMR) spectroscopies. These techniques reveal significant am ounts of bo th secondary and te rtia ry stru ctu re ; the in term ediate differs from a m olten globule in being packed and native-like, b u t it resembles a m olten globule in having no near-ultraviolet (UV) CD spectrum . Final refolding is slow and accom panies proline isom erization. The results show th a t a t least two separate stages are observed in the form ation of the te rtia ry stru ctu re of RNaseS.

Download Full-text

Analysis of vibrational structure in the near-ultraviolet circular dichroism and absorption spectra of phenylalanine and its derivatives

Journal of the American Chemical Society ◽

10.1021/ja01029a034 ◽

1969 ◽

Vol 91 (1) ◽

pp. 184-190 ◽

Cited By ~ 151

Author(s):

Joseph Horwitz ◽

E. Hardin Strickland ◽

Carolyn Billups

Keyword(s):

Circular Dichroism ◽

Absorption Spectra ◽

Vibrational Structure ◽

Near Ultraviolet ◽

Circular Dichroïsm

Download Full-text

Assignment of lectins specific for D-galactose or N-acetyl-D-galactosamine to two groups, based on their circular dichroism

Canadian Journal of Biochemistry and Cell Biology ◽

10.1139/o85-039 ◽

1985 ◽

Vol 63 (4) ◽

pp. 268-271 ◽

Cited By ~ 21

Author(s):

N. Martin Young ◽

Ross E. Williams

Keyword(s):

Circular Dichroism ◽

Ultraviolet Region ◽

Cd Spectra ◽

Difference Spectra ◽

Sophora Japonica ◽

Vicia Villosa ◽

Near Ultraviolet ◽

Caragana Arborescens ◽

Wisteria Floribunda ◽

Circular Dichroïsm

The circular dichroism (CD) spectra of thirteen lectins, most being specific for D-galactose or N-acetyl-D-galactosamine, were compared. Two groupings are proposed on the basis of the CD in the near-ultraviolet region. Group one comprises the lectins from Arachis hypogaea, Glycine max, Phaseolus coccineus, P. lunatus, P. vulgaris, and Vicia villosa, and group two comprises lectins from Caragana arborescens, Cytisus sessilifolius, Dolichos biflorus, Griffonia simplicifolia, and Wisteria floribunda. The CD spectra of lectins from Bauhinia purpurea and Sophora japonica were different from any of the other lectins. CD difference spectra produced by the two sugars were distinctive for each protein, suggesting that the combining sites of these lectins are not homologous despite similarities in specificity and that the CD spectral similarities arise from residues in other, more homologous regions of the proteins.

Download Full-text