Label-free imaging of subcellular features with mid-infrared photothermal microscopy

2020 ◽  
Author(s):  
Michelle Y. Sander ◽  
Panagis Samolis
Keyword(s):  
Label Free ◽  
Mid Infrared ◽  
Photothermal Microscopy

scholarly journals Graphene-Based Biosensors for Detection of Composite Vibrational Fingerprints in the Mid-Infrared Region

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1496
Author(s):  
Yijun Cai ◽  
Yanfen Hang ◽  
Yuanguo Zhou ◽  
Jinfeng Zhu ◽  
Jingwen Yang ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Graphene Nanoribbons ◽  
Infrared Region ◽  
Infrared Range ◽  
Label Free ◽  
Mid Infrared ◽  
Performance Limit ◽  
Wide Range ◽  
Vibrational Signals ◽  
Physical Insight

In this study, a label-free multi-resonant graphene-based biosensor with periodic graphene nanoribbons is proposed for detection of composite vibrational fingerprints in the mid-infrared range. The multiple vibrational signals of biomolecules are simultaneously enhanced and detected by different resonances in the transmission spectrum. Each of the transmission dips can be independently tuned by altering the gating voltage applied on the corresponding graphene nanoribbon. Geometric parameters are investigated and optimized to obtain excellent sensing performance. Limit of detection is also evaluated in an approximation way. Besides, the biosensor can operate in a wide range of incident angles. Electric field intensity distributions are depicted to reveal the physical insight. Moreover, another biosensor based on periodic graphene nanodisks is further proposed, whose performance is insensitive to the polarization of incidence. Our research may have a potential for designing graphene-based biosensor used in many promising bioanalytical and pharmaceutical applications.

Mid-Infrared Nanoplasmonics for Label-free Real-time Biosensing of Proteins and Lipid Membranes

2017 ◽  
Author(s):  
Dordaneh Etezadi ◽  
Odeta Limaj ◽  
Nathan J. Wittenberg ◽  
Daniel Rodrigo ◽  
Daehan Yoo ◽  
...  
Keyword(s):  
Real Time ◽  
Lipid Membranes ◽  
Label Free ◽  
Mid Infrared

Label-free biochemical refractive-index tomography of single cells with mid-infrared photothermal effect

2020 ◽  
Author(s):  
Miu Tamamitsu ◽  
Keiichiro Toda ◽  
Hiroyuki Shimada ◽  
Yu Nagashima ◽  
Ryoichi Horisaki ◽  
...  
Keyword(s):  
Refractive Index ◽  
Single Cells ◽  
Photothermal Effect ◽  
Label Free ◽  
Mid Infrared

Label-free imaging of melanoma with nonlinear photothermal microscopy

2015 ◽  
Vol 40 (7) ◽  
pp. 1141 ◽  
Author(s):  
Jinping He ◽  
Jun Miyazaki ◽  
Nan Wang ◽  
Hiromichi Tsurui ◽  
Takayoshi Kobayashi
Keyword(s):  
Label Free ◽  
Photothermal Microscopy

scholarly journals Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy

2020 ◽  
Vol 25 (10) ◽  
Author(s):  
Yun He ◽  
Junhui Shi ◽  
Miguel A. Pleitez ◽  
Konstantin Maslov ◽  
Daniel A. Wagenaar ◽  
...  
Keyword(s):  
Biological Tissues ◽  
Photoacoustic Microscopy ◽  
Label Free ◽  
Mid Infrared

scholarly journals Mid-infrared optoacoustic microscopy with label-free chemical contrast in living cells and tissues

2018 ◽  
Author(s):  
Miguel A. Pleitez ◽  
Asrar Ali Khan ◽  
Josefine Reber ◽  
Andriy Chmyrov ◽  
Markus R. Seeger ◽  
...  
Keyword(s):  
Imaging Modality ◽  
Living Cells ◽  
Spatiotemporal Dynamics ◽  
Live Cell ◽  
Imaging Modalities ◽  
Label Free ◽  
Infrared Microscopy ◽  
Mid Infrared ◽  
Infrared Sensing ◽  
Biomolecular Imaging

We developed mid-infrared optoacoustic microscopy (MiROM), a bond-selective imaging modality that overcomes water/tissue opacity and depth limitations of mid-infrared sensing allowing uncompromised live-cell/thick-tissue mid-infrared microscopy with up to three orders of magnitudehigher sensitivity than other vibrational imaging modalities; such as Raman. We showcase the functional label-free biomolecular imaging capabilities of MiROM by monitoring the spatiotemporal dynamics of lipids and proteins during lipolysis in living adipocytes. Since MiROM, contrary to Ramanmodalities, is not only able to detect lipids and proteins, but also important metabolites such as glucose without the need of labels, here we discuss how MiROM yields novel functional label-free abilities for a broader range of analytical studies in living cells and tissues.

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