Dealing with reduced data acquisition times in Fluorescence Correlation Spectroscopy (FCS) for High-Throughput Screening (HTS) applications

2003 ◽  
Author(s):  
Lloyd M. Davis ◽  
David A. Ball ◽  
Peter E. Williams ◽  
Kerry M. Swift ◽  
Edmund D. Matayoshi
Keyword(s):  
Data Acquisition ◽  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
High Throughput Screening ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Reduced Data

scholarly journals Single Molecule Detection Technologies in Miniaturized High Throughput Screening: Fluorescence Correlation Spectroscopy

1999 ◽  
Vol 4 (6) ◽  
pp. 335-353 ◽  
Author(s):  
Keith J. Moore ◽  
Sandra Turconi ◽  
Stephen Ashman ◽  
Martin Ruediger ◽  
Ulrich Haupts ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
High Throughput Screening ◽  
Equilibrium Constants ◽  
Correlation Spectroscopy ◽  
Single Molecule Detection ◽  
General Applicability ◽  
Detection Techniques ◽  
Fluorescence Correlation

Fluorescence assay technologies used for miniaturized high throughput screening are broadly divided into two classes. Macroscopic fluorescence techniques (encompassing conventional fluorescence intensity, anisotropy [also often referred to as fluorescence polarization] and energy transfer) monitor the assay volume- and time-averaged fluorescence output from the ensemble of emitting fluorophores. In contrast, single-molecule detection (SMD) techniques and related approaches, such as fluorescence correlation spectroscopy (FCS), stochastically sample the fluorescence properties of individual constituent molecules and only then average many such detection events to define the properties of the assay system as a whole. Analysis of single molecular events is accomplished using confocal optics with an illumination/detection volume of -1 fl (10-15 L) such that the signal is insensitive to miniaturization of HTS assays to 1 A1 or below. In this report we demonstrate the general applicability of one SMD technique (FCS) to assay configuration for target classes typically encountered in HTS and confirm the equivalence of the rate/equilibrium constants determined by FCS and by macroscopic techniques. Advantages and limitations of the current FCS technology, as applied here, and potential solutions, particularly involving alternative SMD detection techniques, are also discussed.

scholarly journals High-throughput screening of optimal solution conditions for structural biological studies by fluorescence correlation spectroscopy

2009 ◽  
Vol 18 (5) ◽  
pp. 1115-1120 ◽  
Author(s):  
Toshihiko Sugiki ◽  
Chie Yoshiura ◽  
Yutaka Kofuku ◽  
Takumi Ueda ◽  
Ichio Shimada ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
High Throughput Screening ◽  
Optimal Solution ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Biological Studies

Synthesis of fluorescent-labeled aeruginosin derivatives for high-throughput fluorescence correlation spectroscopy assays

2007 ◽  
Vol 17 (10) ◽  
pp. 2904-2907 ◽  
Author(s):  
Yoichiro Hoshina ◽  
Yoshifumi Yamada ◽  
Hiroshi Tanaka ◽  
Takayuki Doi ◽  
Takashi Takahashi
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy

2013 ◽  
Vol 455 (3) ◽  
pp. 339-345 ◽  
Author(s):  
Takayasu Mori ◽  
Eriko Kikuchi ◽  
Yuko Watanabe ◽  
Shinya Fujii ◽  
Mari Ishigami-Yuasa ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Library Screening ◽  
Chemical Library ◽  
Fluorescence Correlation ◽  
Fluorescent Correlation Spectroscopy

To discover WNK–OSR1/SPAK signalling inhibitors, we generated a new high-throughput system using fluorescent correlation spectroscopy capable of screening compounds that disrupt the binding of two molecules. We finally identified two novel and promising compounds for WNK–OSR1/SPAK signalling inhibition.

scholarly journals A Rapid and High-Throughput Quantitation Assay of the Nuclear Factor κB Activity Using Fluorescence Correlation Spectroscopy in the Setting of Clinical Laboratories

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e75579 ◽  
Author(s):  
Kenu Harada ◽  
Shintaro Mikuni ◽  
Hideyuki Beppu ◽  
Hideki Niimi ◽  
Shigeki Abe ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Nuclear Factor Κb ◽  
Correlation Spectroscopy ◽  
Nuclear Factor ◽  
Fluorescence Correlation ◽  
Clinical Laboratories

scholarly journals High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles

2020 ◽  
Vol 412 (11) ◽  
pp. 2589-2597 ◽  
Author(s):  
Xu Fu ◽  
Yongwook Song ◽  
Abdullah Masud ◽  
Kanthi Nuti ◽  
Jason E. DeRouchey ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells

2015 ◽  
Vol 33 (4) ◽  
pp. 384-389 ◽  
Author(s):  
Malte Wachsmuth ◽  
Christian Conrad ◽  
Jutta Bulkescher ◽  
Birgit Koch ◽  
Robert Mahen ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Correlation Spectroscopy ◽  
Living Cells ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
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