Regulation of Human Neuronal Calcium Channels by G Protein βγ Subunits Expressed in Human Embryonic Kidney 293 Cells

1997 ◽  
Vol 52 (2) ◽  
pp. 282-291 ◽  
Author(s):  
Lee R. Shekter ◽  
Ronald Taussig ◽  
Samantha E. Gillard ◽  
Richard J. Miller
Keyword(s):  
Calcium Channels ◽  
G Protein ◽  
Human Embryonic Kidney ◽  
293 Cells ◽  
Neuronal Calcium Channels

Endothelin-B receptors activate Gα13

1999 ◽  
Vol 276 (4) ◽  
pp. C930-C937 ◽  
Author(s):  
Kenichiro Kitamura ◽  
Naoki Shiraishi ◽  
William D. Singer ◽  
Mary E. Handlogten ◽  
Kimio Tomita ◽  
...  
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Guanine Nucleotides ◽  
Heterotrimeric G Proteins ◽  
Human Embryonic Kidney ◽  
Cellular Effects ◽  
293 Cells ◽  
Specific Antagonist ◽  
Α Chain ◽  
Endothelin B

Endothelin (ET) receptors activate heterotrimeric G proteins that are members of the Gi, Gq, and Gs families but may also activate members of other families such as Gα12/13. Gα13 has multiple complex cellular effects that are similar to those of ET. We studied the ability of ET receptors to activate Gα13 using an assay for G protein α-chain activation that is based on the fact that an activated (GTP-bound) α-chain is resistant to trypsinization compared with an inactive (GDP-bound) α-chain. Nonhydrolyzable guanine nucleotides and AlMgF protected Gα13 from degradation by trypsin. In membranes from human embryonic kidney 293 cells that coexpress ETB receptors and α13, ET-3 and 5′-guanylylimidodiphosphate [Gpp(NH)p] increased the protection of α13 compared with Gpp(NH)p alone. The specificity of ETBreceptor-α13 coupling was documented by showing that β2receptors and isoproterenol or ETAreceptors and ET-1 did not activate α13 and that a specific antagonist for ETB receptors blocked ET-3-dependent activation of α13.

scholarly journals Determinants of the G protein-dependent opioid modulation of neuronal calcium channels.

1996 ◽  
Vol 93 (4) ◽  
pp. 1486-1491 ◽  
Author(s):  
E. Bourinet ◽  
T. W. Soong ◽  
A. Stea ◽  
T. P. Snutch
Keyword(s):  
Calcium Channels ◽  
G Protein ◽  
Neuronal Calcium Channels

scholarly journals Adenine nucleotides inhibit recombinant N-type calcium channels via G protein-coupled mechanisms in HEK 293 cells; involvement of the P2Y13 receptor-type

2004 ◽  
Vol 141 (1) ◽  
pp. 141-151 ◽  
Author(s):  
Kerstin Wirkner ◽  
Joana Schweigel ◽  
Zoltan Gerevich ◽  
Heike Franke ◽  
Clemens Allgaier ◽  
...  
Keyword(s):  
Calcium Channels ◽  
G Protein ◽  
Adenine Nucleotides ◽  
Receptor Type ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
G Protein Coupled

scholarly journals Selective G-Protein Regulation of Neuronal Calcium Channels

1996 ◽  
Vol 16 (15) ◽  
pp. 4617-4624 ◽  
Author(s):  
Peter T. Toth ◽  
Lee R. Shekter ◽  
Gloria Hui Ma ◽  
Louis H. Philipson ◽  
Richard J. Miller
Keyword(s):  
Calcium Channels ◽  
G Protein ◽  
Protein Regulation ◽  
Neuronal Calcium Channels
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