scholarly journals Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide

2009 ◽  
Vol 75 (9) ◽  
pp. 2940-2944 ◽  
Author(s):  
Sungwoo Bae ◽  
Stefan Wuertz
Keyword(s):  
Quantitative Pcr ◽  
Light Exposure ◽  
Bacteroides Fragilis ◽  
Extracellular Dna ◽  
Propidium Monoazide ◽  
Human Feces ◽  
Heat Treated ◽  
Pcr Conditions

ABSTRACT Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

Quantifying fungal viability in air and water samples using quantitative PCR after treatment with propidium monoazide (PMA)

2008 ◽  
Vol 72 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Stephen Vesper ◽  
Craig McKinstry ◽  
Chris Hartmann ◽  
Michelle Neace ◽  
Stephanie Yoder ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Propidium Monoazide

scholarly journals Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR

2011 ◽  
Vol 85 (2) ◽  
pp. 124-130 ◽  
Author(s):  
M. Adela Yáñez ◽  
Andreas Nocker ◽  
Elena Soria-Soria ◽  
Raquel Múrtula ◽  
Lorena Martínez ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Legionella Pneumophila ◽  
Propidium Monoazide

scholarly journals Application of propidium monoazide coupled with quantitative PCR to evaluate cell viability of Bifidobacterium animalis subsp. lactis in a non-dairy probiotic beverage

2020 ◽  
Vol 70 (1) ◽  
Author(s):  
Carolinne Odebrecht Dias ◽  
Mirella Crhistine Scariot ◽  
Renata Dias de Mello Castanho Amboni ◽  
Ana Carolina Maisonnave Arisi
Keyword(s):  
Cell Viability ◽  
Quantitative Pcr ◽  
Propidium Monoazide ◽  
Bifidobacterium Animalis

scholarly journals Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide-Modified Quantitative PCR

ChemBioChem ◽  
2016 ◽  
Vol 18 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Yu Luo ◽  
Hannah L. Bolt ◽  
Gabriela A. Eggimann ◽  
Danny F. McAuley ◽  
Ronan McMullan ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Propidium Monoazide

Molecular pathogen detection in biosolids with a focus on quantitative PCR using propidium monoazide for viable cell enumeration

2011 ◽  
Vol 87 (3) ◽  
pp. 263-272 ◽  
Author(s):  
Jessica K. van Frankenhuyzen ◽  
Jack T. Trevors ◽  
Hung Lee ◽  
Cecily A. Flemming ◽  
Marc B. Habash
Keyword(s):  
Quantitative Pcr ◽  
Pathogen Detection ◽  
Viable Cell ◽  
Propidium Monoazide ◽  
Cell Enumeration
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