Isolation and Characterization of a Bacillus cereus Mutant Strain Hyperproductive of Exo-β-Amylase

A mutant strain (nf-I) of Hydra magnipapillata was isolated that contained no interstitial cells, nerve cells or nematocytes. This strain appeared spontaneously in a sexually inbred clone of hydra, and it was recognized by its inability to eat. When force-fed, however, it grew, multiplied by budding and regenerated. In this and in many other respects, nf-I was very similar to the interstitial cell-deficient strain produced by Campbell (1976) by means of colchicine. A chimera strain was produced by the reintroduction of interstitial cells from another strain into nf-I. The properties of nf-I, the chimera and other related strains were examined, and the possible roles that the interstitial cells and the nerve cells play in growth and morphogenesis of hydra are discussed.

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Efficient Isolation and Characterization of a Cellulase Hyperproducing Mutant Strain of Trichoderma reesei

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1805.05009 ◽

2018 ◽

Vol 28 (9) ◽

pp. 1473-1481 ◽

Cited By ~ 4

Author(s):

Zongsheng Zou ◽

Yunying Zhao ◽

Tingzhou Zhang ◽

Jiaxing Xu ◽

Aiyong He ◽

...

Keyword(s):

Trichoderma Reesei ◽

Mutant Strain ◽

Isolation And Characterization

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Enumeration, isolation and characterization of Bacillus cereus strains from Spanish raw rice

Food Microbiology ◽

10.1006/fmic.2002.0514 ◽

2002 ◽

Vol 19 (6) ◽

pp. 589-595 ◽

Cited By ~ 42

Author(s):

J.A. Sarrı́as ◽

M. Valero ◽

M.C. Salmerón

Keyword(s):

Bacillus Cereus ◽

Isolation And Characterization

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Isolation and Characterization of the Thylakoid Membranes from the NaCl-Resistant (NaCl r ) Mutant Strain of the Cyanobacterium Anabaena variabilis

Current Microbiology ◽

10.1007/s002840010143 ◽

2000 ◽

Vol 41 (5) ◽

pp. 321-327 ◽

Cited By ~ 4

Author(s):

Vinay S. Chauhan ◽

Bhanumati Singh ◽

Surendra Singh ◽

Rajesh K. Gour ◽

Prakash S. Bisen

Keyword(s):

Mutant Strain ◽

Thylakoid Membranes ◽

Anabaena Variabilis ◽

Isolation And Characterization ◽

Cyanobacterium Anabaena

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Isolation and Characterization of Bacillus cereus Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages

Microorganisms ◽

10.3390/microorganisms8111750 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1750

Author(s):

Chun Li ◽

Xiaoming Yuan ◽

Na Li ◽

Juan Wang ◽

Shubo Yu ◽

...

Keyword(s):

Bacillus Cereus ◽

Dna Packaging ◽

Model Organisms ◽

Experimental Conditions ◽

Isolation And Characterization ◽

Bacillus Phage ◽

Pharmaceutical Industries ◽

High Concentrations ◽

Ph Range

Bacillus phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, named Bacillus cereus phage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most related B. cereus strains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries.

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