Use of Randomly Amplified Polymorphic DNA as a Means of Developing Genus- and Strain-Specific Streptomyces DNA Probes

ABSTRACT We have analyzed 20 randomly amplified polymorphic DNA (RAPD) primers against 36 Streptomyces strains, including 17 taxonomically undefined strains, 25 nonstreptomycete actinomycetes, and 12 outgroups consisting of gram-positive and -negative species. Most of the primers were useful in identifying unique DNA polymorphisms of all strains tested. We have used RAPD techniques to develop a genus-specific probe, one not necessarily targeting the ribosomal gene, for Streptomyces, and a strain-specific probe for the biological control agent Streptomyces lydicus WYEC108. In the course of these investigations, small-scale DNA isolations were also developed for efficiently isolating actinomycete DNA. Various modifications of isolation procedures for soil DNA were compared, and the reliability and specificity of the RAPD methodology were tested by specifically detecting the S. lydicus WYEC108 in DNA isolated from soil.

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Small-scale dispersal of a biological control agent – Implications for more effective releases

Biological Control ◽

10.1016/j.biocontrol.2019.01.016 ◽

2019 ◽

Vol 132 ◽

pp. 89-94 ◽

Cited By ~ 4

Author(s):

Ashley B.C. Goode ◽

Carey R. Minteer ◽

Philip W. Tipping ◽

Brittany K. Knowles ◽

Ryann J. Valmonte ◽

...

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Control Agent ◽

Small Scale

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Biological control of sciarid and phorid pests of mushroom with predatory mites from the genus Hypoaspis (Acari: Hypoaspidae) and the entomopathogenic nematode Steinernema feltiae

Bulletin of Entomological Research ◽

10.1079/ber2003286 ◽

2004 ◽

Vol 94 (2) ◽

pp. 159-167 ◽

Cited By ~ 36

Author(s):

S. Jess ◽

J.F.W. Bingham

Keyword(s):

Biological Control ◽

Biological Control Agent ◽

Entomopathogenic Nematode ◽

Predatory Mites ◽

Mite Species ◽

Control Agent ◽

Steinernema Feltiae ◽

Effective Control ◽

Small Scale ◽

Mushroom Compost

AbstractIn small-scale experiments, the predatory mites, Hypoaspis aculeifer (Canestrini) and H. miles Berlese, applied at 700 mites m−2, and the entomopathogenic nematode, Steinernema feltiae (Filipjev) applied at 3 × 10−6 nematodes m−2 controlled sciarids and phorids in mushroom compost and casing substrates. For both mite species, earliest application to the growing substrate following sciarid infestation reduced sciarid emergence. In contrast, later application of each biological control agent provided more effective control of phorid emergence. The behaviour of adult mites suggested that H. aculeifer were more positively geotactic than H. miles although both species could penetrate compost and casing substrates to a depth of 2–12 cm. A majority of S. feltiae nematodes resided at a depth of 2–4 cm in both substrate types. Independent application of H. aculeifer provided more comprehensive control of sciarids and phorids than the other biological agents studied, owing to its better dispersal within compost and casing, and ability to attack larvae of differing ages.

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