An internet of things framework for real-time aquatic environment monitoring using an Arduino and sensors

Aquaculture is the farming of aquatic organisms in natural, controlled marine and freshwater environments. The real-time monitoring of aquatic environmental parameters is very important in fish farming. Internet of things (IoT) can play a vital role in the real-time monitoring. This paper presents an IoT framework for the efficient monitoring and effective control of different aquatic environmental parameters related to the water. The proposed system is implemented as an embedded system using sensors and an Arduino. Different sensors including pH, temperature, and turbidity, ultrasonic are placed in cultivating pond water and each of them is connected to a common microcontroller board built on an Arduino Uno. The sensors read the data from the water and store it as a comma-separated values (CSV) file in an IoT cloud named ThingSpeak through the Arduino microcontroller. To validate the experiment, we collected data from 5 ponds of various sizes and environments. After experimental evaluation, it was observed among 5 ponds, only three ponds were perfect for fish farming, where these 3 ponds only satisfied the standard reference values of pH (6.5-8.5), temperature (16-24 °C), turbidity (below 10 ntu), conductivity (970-1825 μS/cm), and depth (1-4) meter. At the end of this paper, a complete hardware implementation of this proposed IoT framework for a real-time aquatic environment monitoring system is presented.

Ecological risk factors of soil-transmitted helminths infections in Ifedore district, Southwest Nigeria

Background Soil-Transmitted Helminths are a group of parasites that cause gastrointestinal infections in humans and require the soil to develop into their infective forms. Ecological factors such as soil temperature, soil pH and rainfall patterns are, however, important determinants for the successful transmission of soil helminths as they play a major role in their abundance and survival in the soil. The study investigated the ecological factors influencing the transmission of soil-transmitted helminths in Ifedore district, Southwest Nigeria. Results Out of the one hundred and ninety-two (192) soil samples from the study area, one hundred and fifty-two 152 (79.2%) were positive for the presence of soil helminths’ larvae and ova. Higher occurrence of soil helminths was recorded during the rainy months (n = 416) than during the months with no records of rainfall (n = 290). Sandy soil had the highest number of soil helminths 285 (40.4%), while clay soil recorded the least 88 (12.5%). Soil temperature showed negative correlations with the occurrence of Ancylostoma duodenale (r = − 0.53) and Strongyloides stercoralis larvae (r = − 0.36), while soil conductivity showed positive correlations with the occurrence of Ascaris lumbricoides (r = 0.28) and A. duodenale (r = 0.34). Conclusion It is evident from the study that ecological factors played a significant role in the occurrence and abundance of soil-transmitted helminths. This research is important for predicting and monitoring soil-transmitted helminthiasis in endemic countries, and to devise effective control measures.

Numerical and experimental analysis on the helicopter rotor dynamic load controlled by the actively trailing edge flap

Reducing the rotor dynamic load is an important issue to improve the performance and reliability of a helicopter. The control mechanism of the actively controlled flap on the rotor dynamic load is numerically and experimentally investigated by a 3-blade helicopter rotor in this paper. In the aero-elastic numerical approach, the complex motion of the rotor such as the stretching, bending, torsion and pitching of the blade including the deflection of the actively controlled flap (ACF) are all taken into consideration in the structural formulation. The aerodynamic solution adopted the vortex lattice method combining with the free wake model, in which the influence of ACF on the free wake and the aerodynamic load on the blade is taken into account as well. While the experimental method of measuring hub loads and acoustic was accomplished by a rotor rig in a wind tunnel. The result shows that the 3/rev ACF actuation can reduce the $3\omega$ hub load by more than 50\% at maximum, which is significantly better than the 4/rev control. While 4/rev has greater potential to reduce BVI loads than 3/rev with $\mu=0.15$. Further mechanistic analysis shows that by changing the phase difference between the dynamic load on the flap and the rest of the blade, the peak load on the whole blade can be improved, thus achieving effective control of the hub dynamic load, the flap reaches the minimum angle of attack at 90°-100° azimuth under best control condition; when the BVI load is perfectly controlled, the flap reaches the minimum angle of attack at 140° azimuth, and by changing the circulation of the wake, the intensity of blade vortex interaction in the advancing side is improved. Moreover, an interesting finding in the optimal control of noise and vibration is that an overlap point exist on the motion patterns of the flap with different frequencies.

The Conserved Macrodomain Is a Potential Therapeutic Target for Coronaviruses and Alphaviruses

Emerging and re-emerging viral diseases pose continuous public health threats, and effective control requires a combination of non-pharmacologic interventions, treatment with antivirals, and prevention with vaccines. The COVID-19 pandemic has demonstrated that the world was least prepared to provide effective treatments. This lack of preparedness has been due, in large part, to a lack of investment in developing a diverse portfolio of antiviral agents, particularly those ready to combat viruses of pandemic potential. Here, we focus on a drug target called macrodomain that is critical for the replication and pathogenesis of alphaviruses and coronaviruses. Some mutations in alphavirus and coronaviral macrodomains are not tolerated for virus replication. In addition, the coronavirus macrodomain suppresses host interferon responses. Therefore, macrodomain inhibitors have the potential to block virus replication and restore the host’s protective interferon response. Viral macrodomains offer an attractive antiviral target for developing direct acting antivirals because they are highly conserved and have a structurally well-defined (druggable) binding pocket. Given that this target is distinct from the existing RNA polymerase and protease targets, a macrodomain inhibitor may complement current approaches, pre-empt the threat of resistance and offer opportunities to develop combination therapies for combating COVID-19 and future viral threats.

Identification and Expression Profile of Chemosensory Receptor Genes in Aromia bungii (Faldermann) Antennal Transcriptome

The red-necked longicorn beetle, Aromia bungii (Faldermann) (Coleoptera: Cerambycidae), is a major destructive, wood-boring pest, which is widespread throughout the world. The sex pheromone of A. bungii was reported earlier; however, the chemosensory mechanism of the beetle remains almost unknown. In this study, 45 AbunORs, 6 AbunGRs and 2 AbunIRs were identified among 42,197 unigenes derived from the antennal transcriptome bioinformatic analysis of A. bungii adults. The sequence of putative Orco (AbunOR25) found in this study is highly conserved with the known Orcos from other Coleoptera species, and these Orco genes might be potentially used as target genes for the future development of novel and effective control strategies. Tissue expression analysis showed that 29 AbunOR genes were highly expressed in antennae, especially in the antennae of females, which was consistent with the idea that females might express more pheromone receptors for sensing pheromones, especially the sex pheromones produced by males. AbunOR5, 29, 31 and 37 were clustered with the pheromone receptors of the cerambycid Megacyllene caryae, suggesting that they might be putative pheromone receptors of A. bungii. All six AbunGRs were highly expressed in the mouthparts, indicating that these GRs may be involved in the taste perception process. Both AbunIRs were shown to be female-mouthparts-biased, suggesting that they might also be related to the tasting processes. Our study provides some basic information towards a deeper understanding of the chemosensing mechanism of A. bungii at a molecular level.

The Fight against Corruption in Sport: International and National Experience

Corruption is a particularly dangerous scourge, which encompasses the whole of society, all spheres of human activity, and by virtue of this, it also encompasses education, whether we want to recognize it or not. Corruption flourishes in times of great social unrest, in times of crisis that societies are going through, especially in the period of transition from a totalitarian regime to a democratic society. The factor generating corruption is the socio-economic crisis, having as causes: weakening of state authority, degradation of living standards, altered moral judgment, lack of effective control levers, diminished public confidence in institutions and social values, non-adaptation of legislation to economic and social conditions and so on Sport is one of the largest businesses in the world, being influenced and influencing in turn both financial and political interests. Every year, millions of dollars and Euros circulate in this area, most of the transactions and agreements taking place behind closed doors, in order to keep any possible advantage over the competition. This fierce competitiveness, together with the lack of transparency, makes the sports field extremely vulnerable to corruption acts. Summarizing the results of our study, we can conclude: the fight against corruption is an opportunity for beneficiary institutions, which can strengthen and improve their systems for preventing and combating corruption and money laundering and asset recovery, by reference to worldwide first-class practices and standards. Following intense consultations with all beneficiaries in the Republic of Moldova: Minister of Education, Culture and Research, National Olympic and Sports Committee, Paralympics’ Committee, Sports Federations and other structures in the country, in order to ensure effective support in the field of physical culture and sports. The direct relations with the interested actors contribute to the creation of a positive framework, which will favour the general success of the future activities that will take place in obtaining the expected results.

The world’s first glyphosate-resistant case of Avena fatua L. and Avena sterilis ssp. ludoviciana (Durieu) Gillet & Magne and alternative herbicide options for their control

Avena fatua and A. ludoviciana (commonly known as wild oats) are the most problematic winter grass species in fallows and winter crops in the northeast region of Australia. A series of experiments were conducted to evaluate the performance of glyphosate and alternative post-emergence herbicides on A. fatua and A. ludoviciana. This study reports the world’s first glyphosate-resistant (GR) biotypes of A. fatua and A. ludoviciana. The glyphosate dose required to kill 50% of the plants (LD50) and to reduce 50% of the biomass (GR50) for the GR biotype of A. fatua was 556 g a.e./ha and 351 g a.e./ha, respectively. These values for A. ludoviciana were 848 g a.e./ha and 289 g a.e./ha. Regardless of the growth stage (3–4 or 6–7 leaf stages), clethodim (120 g a.i./ha), haloxyfop (78 g a.i./ha), pinoxaden (20 g a.i./ha), and propaquizafop (30 g a.i./ha) were the best alternative herbicide options for the control of A. fatua and A. ludoviciana. The efficacy of butroxydim (45 g a.i./ha), clodinafop (120 g a.i./ha), imazamox + imazapyr (36 g a.i./ha), and paraquat (600 g a.i./ha) reduced at the advanced growth stage. Glufosinate (750 g a.i./ha), flamprop (225 g a.i./ha), and pyroxsulam + halauxifen (20 g a.i./ha) did not provide effective control of Avena species. This study identified alternative herbicide options to manage GR biotypes of A. fatua and A. ludoviciana.

Exploring the Effects of Different Stubble Tillage Practices and Glyphosate Application Combined with the New Soil Residual Herbicide Cinmethylin against Alopecurus myosuroides Huds. in Winter Wheat

Effective control of Alopecurus myosuroides Huds. (blackgrass) solely with a chemical treatment is not guaranteed anymore because populations exhibit resistance to almost all herbicide modes of action. Integrated weed management (IWM) against blackgrass is necessary to maintain high weed control efficacies in winter cereals. Four field experiments were conducted in Southwest Germany from 2018 to 2020 to control A. myosuroides with a combination of cultural and chemical methods. Stubble treatments, including flat, deep and inversion soil tillage; false seedbed preparation and glyphosate use, were combined with the application of the new pre-emergence herbicide cinmethylin in two rates in winter wheat. Average densities of A. myosuroides in the untreated control plots were up to 505 plants m−2. The combination of different stubble management strategies and the pre-emergence herbicide cinmethylin controlled 86–97% of A. myosuroides plants at the low rate and 95–100% at the high rate until 120 days after sowing. The different stubble tillage practices varied in their efficacy between trials and years. Most effective and consistent were pre-sowing glyphosate application on the stubble and stale seedbed preparation with a disc harrow. Stubble treatments increased winter wheat density in the first year but had no effect on crop density in the second year. Pre-emergence application of cinmethylin did not reduce winter wheat densities. Multiple tactics of weed control, including stubble treatments and pre-emergence application of cinmethylin, provided higher and more consistent control of A. myosuroides. Integration of cultural weed management could prevent the herbicide resistance development.

Inactivation of Spores and Vegetative Forms of Clostridioides Difficile by Chemical Biocides: Mechanisms of Biocidal Activity, Methods of Evaluation, and Environmental Aspects

Clostridioides difficile infections (CDIs) are the most common cause of acquired diseases in hospitalized patients. Effective surface disinfection, focused on the inactivation of the spores of this pathogen, is a decisive factor in reducing the number of nosocomial cases of CDI infections. An efficient disinfection procedure is the result of both the properties of the biocidal agent used and the technology of its implementation as well as a reliable, experimental methodology for assessing the activity of the biocidal active substance based on laboratory models that adequately represent real clinical conditions. This study reviews the state of knowledge regarding the properties and biochemical basis of the action mechanisms of sporicidal substances, with emphasis on chlorine dioxide (ClO2). Among the analyzed biocides, in addition to ClO2, active chlorine, hydrogen peroxide, peracetic acid, and glutaraldehyde were characterized. Due to the relatively high sporicidal effectiveness and effective control of bacterial biofilm, as well as safety in a health and environmental context, the use of ClO2 is an attractive alternative in the control of nosocomial infections of CD etiology. In terms of the methods of assessing the biocidal effectiveness, suspension and carrier standards are discussed.

When stem cells meet COVID-19: recent advances, challenges and future perspectives

Coronavirus disease 2019 (COVID-19) caused by the novel severe acute respiratory coronavirus 2 is currently spreading throughout the world with a high rate of infection and mortality and poses a huge threat to global public health. COVID-19 primarily manifests as hypoxic respiratory failure and acute respiratory distress syndrome, which can lead to multiple organ failure. Despite advances in the supportive care approaches, there is still a lack of clinically effective therapies, and there is an urgent need to develop novel strategies to fight this disease. Currently, stem cell therapy and stem cell-derived organoid models have received extensive attention as a new treatment and research method for COVID-19. Here, we discuss how stem cells play a role in the battle against COVID-19 and present a systematic review and prospective of the study on stem cell treatment and organoid models of COVID-19, which provides a reference for the effective control of the COVID-19 pandemic worldwide.