Toxicity Analysis of N- and C-Terminus-Deleted Vegetative Insecticidal Protein from Bacillus thuringiensis

ABSTRACT A vegetative insecticidal protein (VIP)-encoding gene from a local isolate of Bacillus thuringiensis has been cloned, sequenced, and expressed in Escherichia coli. The expressed protein shows insecticidal activity against several lepidopteran pests but is ineffective against Agrotis ipsilon. Comparison of the amino acid sequence with those of reported VIPs revealed a few differences. Analysis of insecticidal activity with N- and C-terminus deletion mutants suggests a differential mode of action of VIP against different pests.

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Heterologous Expression of Bacillus thuringiensis Vegetative Insecticidal Protein-Encoding Gene vip3LB in Photorhabdus temperata Strain K122 and Oral Toxicity against the Lepidoptera Ephestia kuehniella and Spodoptera littoralis

Molecular Biotechnology ◽

10.1007/s12033-009-9179-3 ◽

2009 ◽

Vol 43 (2) ◽

pp. 97-103 ◽

Cited By ~ 9

Author(s):

Kaïs Jamoussi ◽

Sameh Sellami ◽

Lobna Abdelkefi-Mesrati ◽

Alain Givaudan ◽

Samir Jaoua

Keyword(s):

Bacillus Thuringiensis ◽

Heterologous Expression ◽

Spodoptera Littoralis ◽

Ephestia Kuehniella ◽

Insecticidal Protein ◽

Oral Toxicity ◽

Vegetative Insecticidal Protein ◽

Protein Encoding ◽

Encoding Gene ◽

Photorhabdus Temperata

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Survivorship of Helicoverpa zea and Heliothis virescens on Cotton Plant Structures Expressing a Bacillus thuringiensis Vegetative Insecticidal Protein

Journal of Economic Entomology ◽

10.1093/jee/101.4.1244 ◽

2008 ◽

Vol 101 (4) ◽

pp. 1244-1252 ◽

Cited By ~ 5

Author(s):

P. L. Bommireddy ◽

B. R. Leonard

Keyword(s):

Bacillus Thuringiensis ◽

Cotton Plant ◽

Heliothis Virescens ◽

Helicoverpa Zea ◽

Insecticidal Protein ◽

Vegetative Insecticidal Protein

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Carboxy-terminal half of Cry1C can help vegetative insecticidal protein to form inclusion bodies in the mother cell of Bacillus thuringiensis

Applied Microbiology and Biotechnology ◽

10.1007/s00253-008-1613-0 ◽

2008 ◽

Vol 80 (4) ◽

pp. 647-654 ◽

Cited By ~ 13

Author(s):

Rong Song ◽

Donghai Peng ◽

Ziniu Yu ◽

Ming Sun

Keyword(s):

Bacillus Thuringiensis ◽

Mother Cell ◽

Inclusion Bodies ◽

Insecticidal Protein ◽

Vegetative Insecticidal Protein ◽

Carboxy Terminal

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Vegetative Insecticidal Protein (Vip): A Potential Contender From Bacillus thuringiensis for Efficient Management of Various Detrimental Agricultural Pests

Frontiers in Microbiology ◽

10.3389/fmicb.2021.659736 ◽

2021 ◽

Vol 12 ◽

Author(s):

Mamta Gupta ◽

Harish Kumar ◽

Sarvjeet Kaur

Keyword(s):

Bacillus Thuringiensis ◽

Insecticidal Protein ◽

Current Status ◽

Specific Class ◽

Agricultural Pests ◽

Cry Proteins ◽

Vegetative Insecticidal Protein ◽

Receptor Sites ◽

Vip Proteins ◽

The Difference

Bacillus thuringiensis (Bt) bacterium is found in various ecological habitats, and has natural entomo-pesticidal properties, due to the production of crystalline and soluble proteins during different growth phases. In addition to Cry and Cyt proteins, this bacterium also produces Vegetative insecticidal protein (Vip) during its vegetative growth phase, which is considered an excellent toxic candidate because of the difference in sequence homology and receptor sites from Cry proteins. Vip proteins are referred as second-generation insecticidal proteins, which can be used either alone or in complementarity with Cry proteins for the management of various detrimental pests. Among these Vip proteins, Vip1 and Vip2 act as binary toxins and have toxicity toward pests belonging to Hemiptera and Coleoptera orders, whereas the most important Vip3 proteins have insecticidal activity against Lepidopteran pests. These Vip3 proteins are similar to Cry proteins in terms of toxicity potential against susceptible insects. They are reported to be toxic toward pests, which can’t be controlled with Cry proteins. The Vip3 proteins have been successfully pyramided along with Cry proteins in transgenic rice, corn, and cotton to combat resistant pest populations. This review provides detailed information about the history and importance of Vip proteins, their types, structure, newly identified specific receptors, and action mechanism of this specific class of proteins. Various studies conducted on Vip proteins all over the world and the current status have been discussed. This review will give insights into the significance of Vip proteins as alternative promising candidate toxic proteins from Bt for the management of pests in most sustainable manner.

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